KDM2A Antibody (YA2812)(PBS only)

Cat. No.: HY-P83067A: Data Sheet User Guide for Antibodies
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KDM2A Antibody (YA2812) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to KDM2A.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

KDM2A Antibody (YA2812) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to KDM2A.

Host

Rabbit

Molecular Weight

Predicted band size: 133 kDa;

Observed band size: 133 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

Q9Y2K7

Gene ID

22992 [NCBI]

Immunogen

A synthetic peptide of human FBXL11 aa2-19.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:50-1:100
IHC-F IHC-F: Immunohistochemistry-Frozen	1:50-1:100
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:50-1:200
IP IP: Immunoprecipitation	1:20

Purity	Affinity Purified	Conjugation	Non-conjugated
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Histone demethylase that specifically demethylates 'Lys-36' of histone H3, thereby playing a central role in histone code. Preferentially demethylates dimethylated H3 'Lys-36' residue while it has weak or no activity for mono- and tri-methylated H3 'Lys-36'. May also recognize and bind to some phosphorylated proteins and promote their ubiquitination and degradation. Required to maintain the heterochromatic state. Associates with centromeres and represses transcription of small non-coding RNAs that are encoded by the clusters of satellite repeats at the centromere. Required to sustain centromeric integrity and genomic stability, particularly during mitosis. Regulates circadian gene expression by repressing the transcriptional activator activity of CLOCK-BMAL1 heterodimer and RORA in a catalytically-independent manner (PubMed:26037310)

Subcellular Localization：Nucleus, nucleoplasm; Chromosome

Expression：
Tissue_specificity:Widely expressed, with highest levels in brain, testis and ovary, followed by lung

Isoforms & Post-Translational Modification：Q9Y2K7 has 5 isomers: Q9Y2K7-1: 132793 Da (predicted); Q9Y2K7-2: 96859 Da (predicted); Q9Y2K7-4: 69898 Da (predicted); Q9Y2K7-5: 81678 Da (predicted); Q9Y2K7-3: 89948 Da (predicted).
Mono-ADP-ribosylated at Arg-1020 in response to DNA damage, leading to displacement from chromatin, resulting in increased dimethylation of histone H3 at 'Lys-36'

Subunit：Interacts with CBX5/HP1A; the interaction promotes CBX5 localization to chromatin (PubMed:19001877). The SKP1-KDM2A complex interacts with UBB (PubMed:30033217). Part of a SCF (SKP1-cullin-F-box) protein ligase complex (PubMed:30033217)

Research Field

Epigenetics and Nuclear Signaling

Synonyms

FBL7; CXXC8; FBL11; FBXL11; JHDM1A; LILINA

Documentation

User Guide for Antibodies (1077 KB)