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PCCA Antibody (YA7884)

(Synonyms: PCCase subunit alpha, Propanoyl-CoA:carbon dioxide ligase subunit alpha, PCCA)
Customer Review

Based on 1 Customer Validation

PCCA Antibody (YA7884) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to PCCA.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG

  • Application:

    WB, IHC-P

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Spplied in PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

  • Conjugation:
    Non-conjugated
IHC-P
  • Experimental Validation Results for PCCA Antibody (YA7884)
  • Experimental Validation Results for PCCA Antibody (YA7884)
  • Experimental Validation Results for PCCA Antibody (YA7884)
  • Experimental Validation Results for PCCA Antibody (YA7884)
  • Experimental Validation Results for PCCA Antibody (YA7884)
  • PCCA Antibody (YA7884) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

Service
Data Download
Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
Dilution Ratio 1:500-2000 1:50-5000

Product Details

Description

PCCA Antibody (YA7884) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to PCCA.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Calculated Molecular Weight Predicted band size: 80.06 kDa
Immunogen

Full length human recombiant protein of human PCCA produced in HEK293T cell.

Sensitivity

Endogenous

Purification

Affinity purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Solution

  • Formulation

    Spplied in PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for PCCA Antibody (YA7884)
    Immunohistochemical analysis of paraffin-embedded rat liver tissue using PCCA Antibody (HY-P88200, 1/2000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for PCCA Antibody (YA7884)
    Immunohistochemical analysis of paraffin-embedded rat pancreas tissue using PCCA Antibody (HY-P88200, 1/2000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for PCCA Antibody (YA7884)
    Immunohistochemical analysis of paraffin-embedded rat thyroid gland tissue using PCCA Antibody (HY-P88200, 1/2000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for PCCA Antibody (YA7884)
    Immunohistochemical analysis of paraffin-embedded rat adrenal gland tissue using PCCA Antibody (HY-P88200, 1/2000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for PCCA Antibody (YA7884)
    Immunohistochemical analysis of paraffin-embedded human salivary gland tissue using PCCA Antibody (HY-P88200, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Background

  • Function

    PCCA is a This is one of the 2 subunits of the biotin-dependent propionyl-CoA carboxylase (PCC), a mitochondrial enzyme involved in the catabolism of odd chain fatty acids, branched-chain amino acids isoleucine, threonine, methionine, and valine and other metabolites. Propionyl-CoA carboxylase catalyzes the carboxylation of propionyl-CoA/propanoyl-CoA to D-methylmalonyl-CoA/(S)-methylmalonyl-CoA. Within the holoenzyme, the alpha subunit catalyzes the ATP-dependent carboxylation of the biotin carried by the biotin carboxyl carrier (BCC) domain, while the beta subunit then transfers the carboxyl group from carboxylated biotin to propionyl-CoA. Propionyl-CoA carboxylase also significantly acts on butyryl-CoA/butanoyl-CoA, which is converted to ethylmalonyl-CoA/(2S)-ethylmalonyl-CoA at a much lower rate. Other alternative minor substrates include (2E)-butenoyl-CoA/crotonoyl-CoA[1][2][3].

  • Subcellular Localization

    Mitochondrion matrix

  • Isoforms & Post-Translational Modification

    P05165 has three isomers: P05165-1: 80059 Da (predicted); P05165-2: 77048 Da (predicted); P05165-3: 75002 Da (predicted).
    Acetylated丨The biotin cofactor is covalently attached to the C-terminal biotinyl-binding domain and is required for the catalytic activity (PubMed:10329019)

  • Subunit

    The holoenzyme is a dodecamer composed of 6 PCCA/alpha subunits and 6 PCCB/beta subunits (PubMed:20725044, PubMed:6765947)

  • SwissProt ID

    P05165

  • Synonyms

    PCCase subunit alpha, Propanoyl-CoA:carbon dioxide ligase subunit alpha, PCCA

PCCA Antibody (YA7884) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded rat liver tissue using PCCA Antibody (HY-P88200, 1/2000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded rat pancreas tissue using PCCA Antibody (HY-P88200, 1/2000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded rat thyroid gland tissue using PCCA Antibody (HY-P88200, 1/2000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded rat adrenal gland tissue using PCCA Antibody (HY-P88200, 1/2000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human salivary gland tissue using PCCA Antibody (HY-P88200, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • PCCA Antibody (YA7884) Product Image
    1/16
  • Experimental Validation Results for PCCA Antibody (YA7884)
  • Experimental Validation Results for PCCA Antibody (YA7884)
  • Experimental Validation Results for PCCA Antibody (YA7884)
  • Experimental Validation Results for PCCA Antibody (YA7884)
  • Experimental Validation Results for PCCA Antibody (YA7884)
  • PCCA Antibody (YA7884) Product Image
  • Experimental Validation Results for PCCA Antibody (YA7884)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded rat liver tissue using PCCA Antibody (HY-P88200, 1/2000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for PCCA Antibody (YA7884)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded rat pancreas tissue using PCCA Antibody (HY-P88200, 1/2000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for PCCA Antibody (YA7884)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded rat thyroid gland tissue using PCCA Antibody (HY-P88200, 1/2000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for PCCA Antibody (YA7884)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded rat adrenal gland tissue using PCCA Antibody (HY-P88200, 1/2000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for PCCA Antibody (YA7884)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human salivary gland tissue using PCCA Antibody (HY-P88200, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for PCCA Antibody (YA7884)
  • Experimental Validation Results for PCCA Antibody (YA7884)
  • Experimental Validation Results for PCCA Antibody (YA7884)
  • Experimental Validation Results for PCCA Antibody (YA7884)
  • Experimental Validation Results for PCCA Antibody (YA7884)
  • PCCA Antibody (YA7884) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded rat liver tissue using PCCA Antibody (HY-P88200, 1/2000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • PCCA Antibody (YA7884) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded rat pancreas tissue using PCCA Antibody (HY-P88200, 1/2000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • PCCA Antibody (YA7884) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded rat thyroid gland tissue using PCCA Antibody (HY-P88200, 1/2000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • PCCA Antibody (YA7884) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded rat adrenal gland tissue using PCCA Antibody (HY-P88200, 1/2000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • PCCA Antibody (YA7884) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human salivary gland tissue using PCCA Antibody (HY-P88200, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • PCCA Antibody (YA7884) Product Image IHC-P
  • PCCA Antibody (YA7884) Product Image IHC-P
  • PCCA Antibody (YA7884) Product Image IHC-P
  • PCCA Antibody (YA7884) Product Image IHC-P
  • PCCA Antibody (YA7884) Product Image IHC-P