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GFPT1 Antibody (YA2912)(PBS only)

(Synonyms: GFA; GFAT; GFPT; MSLG; CMS12; GFAT1; CMSTA1; GFAT 1; GFAT1m; GFPT1L)

GFPT1 Antibody (YA2912) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to GFPT1.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, IP

  • Reactivity :

    Human

  • Formulation:

    Supplied in PBS, pH 7.4.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for GFPT1 Antibody (YA2912)(PBS only)
  • GFPT1 Antibody (YA2912)(PBS only) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:500-1:1000 1:50-1:100 1:20

Product Details

Description

GFPT1 Antibody (YA2912) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to GFPT1.

  • Host Rabbit
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 79 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 79 kDa
Immunogen

A synthetic peptide of human GFPT1

Purification

Affinity Purified

Conjugation

Non-conjugated

Isotype

IgG

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in PBS, pH 7.4.

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for GFPT1 Antibody (YA2912)(PBS only)
    Western blot analysis of extracts from Hela(lane 2(20ug) and Hela(lane 3(40ug) using GFPT1 Antibody (HY-P831670) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

Background

  • Function

    GFPT1 controls the flux of glucose into the hexosamine pathway. Most likely involved in regulating the availability of precursors for N- and O-linked glycosylation of proteins. Regulates the circadian expression of clock genes BMAL1 and CRY1. Has a role in fine tuning the metabolic fluctuations of cytosolic UDP-GlcNAc and its effects on hyaluronan synthesis that occur during tissue remodeling[1].

  • Expression


    Tissue_specificity:Isoform1is predominantly expressed in skeletal muscle. Not expressed in brain. Seems to be selectively expressed in striated muscle.

  • Isoforms & Post-Translational Modification

    Q06210 has 2 isomers: Q06210-1: 78806 Da (predicted); Q06210-2: 76759 Da (predicted).

  • Subunit

    Homotetramer (Probable), may also exist as homodimers

  • SwissProt ID

    Q06210

  • Gene ID
    2673 [NCBI]
  • Synonyms

    GFA; GFAT; GFPT; MSLG; CMS12; GFAT1; CMSTA1; GFAT 1; GFAT1m; GFPT1L

  • Research Field

    Signal Transduction

GFPT1 Antibody (YA2912)(PBS only) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis of extracts from Hela(lane 2(20ug) and Hela(lane 3(40ug) using GFPT1 Antibody (HY-P831670) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • GFPT1 Antibody (YA2912)(PBS only) Product Image
    1/16
  • Experimental Validation Results for GFPT1 Antibody (YA2912)(PBS only)
  • GFPT1 Antibody (YA2912)(PBS only) Product Image
  • Experimental Validation Results for GFPT1 Antibody (YA2912)(PBS only)
    1/1
    MedchemExpress Validation
     Western blot analysis of extracts from Hela(lane 2(20ug) and Hela(lane 3(40ug) using GFPT1 Antibody (HY-P831670) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for GFPT1 Antibody (YA2912)(PBS only)
  • GFPT1 Antibody (YA2912)(PBS only) Product Image
    MedchemExpress Validation

    Western blot analysis of extracts from Hela(lane 2(20ug) and Hela(lane 3(40ug) using GFPT1 Antibody (HY-P831670) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • GFPT1 Antibody (YA2912)(PBS only) Product Image WB