CSB Antibody (YA8912)
(Synonyms: CSB, ERCC6, DNA excision repair protein ERCC-6, ATP-dependent helicase ERCC6, Cockayne syndrome protein CSB)
Based on 1 Customer Validation
CSB Antibody (YA8912) is a Mouse-derived and non-conjugated IgG2b monoclonal antibody, targeting to CSB.
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Host:
Mouse
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Isotype:
IgG
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Application:
WB, ICC/IF, IF-Tissue, IP, ELISA
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Reactivity :
human
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Formulation:
Supplied in PBS(pH7.4) containing 0.1% gelatin and < 0.1% sodium azide.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
IF-Tissue
IF-Tissue: Immunofluorescence-Tissue
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IP
IP: Immunoprecipitation
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ELISA
ELISA: Enzyme Linked Immunosorbent Assay
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|---|---|---|---|---|---|
| Dilution Ratio | 1:500-1000 | 1:50-500 | 1:50-500 | 1-2μg per 100-500μg Total protein | 1:50-3000 |
Product Details
CSB Antibody (YA8912) is a Mouse-derived and non-conjugated IgG2b monoclonal antibody, targeting to CSB.
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Host Mouse
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Species Reactivityhuman
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Calculated Molecular Weight Predicted band size: 168 kDa
OMIM: 609413
A synthesized peptide derived from human CSB.
Endogenous
Affinity purified
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in PBS(pH7.4) containing 0.1% gelatin and < 0.1% sodium azide.
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Concentration
Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration
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Storage & Stability
Stored at 2-8°C for 1 year, do not freeze.
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Shipping
Shipping with blue ice.
Background
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Function
CSB is essential factor involved in transcription-coupled nucleotide excision repair (TC-NER), a process during which RNA polymerase II-blocking lesions are rapidly removed from the transcribed strand of active genes. Plays a central role in the initiation of the TC-NER process: specifically recognizes and binds RNA polymerase II stalled at a lesion, and mediates recruitment of ERCC8/CSA, initiating DNA damage excision by TFIIH recruitment. Upon DNA-binding, it locally modifies DNA conformation by wrapping the DNA around itself, thereby modifying the interface between stalled RNA polymerase II and DNA. Acts as a chromatin remodeler at DSBs; DNA-dependent ATPase-dependent activity is essential for this function. Plays an important role in regulating the choice of the DNA double-strand breaks (DSBs) repair pathway and G2/M checkpoint activation; DNA-dependent ATPase activity is essential for this function. Regulates the DNA repair pathway choice by inhibiting non-homologous end joining (NHEJ), thereby promoting the homologous recombination (HR)-mediated repair of DSBs during the S/G2 phases of the cell cycle. Mediates the activation of the ATM- and CHEK2-dependent DNA damage responses thus preventing premature entry of cells into mitosis following the induction of DNA DSBs. Remodels chromatin by evicting histones from chromatin flanking DSBs, limiting RIF1 accumulation at DSBs thereby promoting BRCA1-mediated HR. Required for stable recruitment of ELOA and CUL5 to DNA damage sites. Also involved in UV-induced translocation of ERCC8 to the nuclear matrix. Essential for neuronal differentiation and neuritogenesis; regulates transcription and chromatin remodeling activities required during neurogenesis[1][2][3][4][5][6][7][8][9][10][11][12][13][14][15].
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Subcellular Localization
Nucleus; Chromosome
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Isoforms & Post-Translational Modification
CSB has 2 isoforms, Q03468-1: amino acid length is 1493, molecular weight is 168416 Da (predicted); P0DP91-1: amino acid length is 1061, molecular weight is 119487 Da (predicted).
Phosphorylated in a cell cycle-dependent manner at Ser-158 by cyclin A-CDK2 and at Ser-10 by ATM in response to DNA damage. Phosphorylation at these two sites promotes the intramolecular interaction of the N-terminal domain with the helicase ATP-binding domain, thereby probably releasing the inhibitory effect of the N-terminal domain on its ATPase activity. Phosphorylation is essential for its chromatin remodeling activity. -
Subunit
Homodimer.
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SwissProt ID
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Synonyms
CSB, ERCC6, DNA excision repair protein ERCC-6, ATP-dependent helicase ERCC6, Cockayne syndrome protein CSB
Documentation
[1]. Sarker AH, et al. Recognition of RNA polymerase II and transcription bubbles by XPG, CSB, and TFIIH: insights for transcription-coupled repair and Cockayne Syndrome. Mol Cell. 2005 Oct 28;20(2):187-98. [Content Brief]
[2]. Anindya R, et al. A ubiquitin-binding domain in Cockayne syndrome B required for transcription-coupled nucleotide excision repair. Mol Cell. 2010 Jun 11;38(5):637-48. [Content Brief]
[3]. Bailey AD, et al. The conserved Cockayne syndrome B-piggyBac fusion protein (CSB-PGBD3) affects DNA repair and induces both interferon-like and innate antiviral responses in CSB-null cells. DNA Repair (Amst). 2012 May 1;11(5):488-501. [Content Brief]
[4]. Sin Y, et al. The C-terminal Region and SUMOylation of Cockayne Syndrome Group B Protein Play Critical Roles in Transcription-coupled Nucleotide Excision Repair. J Biol Chem. 2016 Jan 15;291(3):1387-97. [Content Brief]
[5]. van der Weegen Y, et al. The cooperative action of CSB, CSA, and UVSSA target TFIIH to DNA damage-stalled RNA polymerase II. Nat Commun. 2020 Apr 30;11(1):2104. [Content Brief]
[6]. Kokic G, et al. Structural basis of human transcription-DNA repair coupling. Nature. 2021 Oct;598(7880):368-372. [Content Brief]
[7]. Kokic G, et al. Structural basis for RNA polymerase II ubiquitylation and inactivation in transcription-coupled repair. Nat Struct Mol Biol. 2024 Mar;31(3):536-547. [Content Brief]
[8]. Carnie CJ, et al. Transcription-coupled repair of DNA-protein cross-links depends on CSA and CSB. Nat Cell Biol. 2024 May;26(5):797-810. [Content Brief]
[9]. van Sluis M, et al. Transcription-coupled DNA-protein crosslink repair by CSB and CRL4(CSA)-mediated degradation. Nat Cell Biol. 2024 May;26(5):770-783. [Content Brief]
[10]. Beerens N, et al. The CSB protein actively wraps DNA. J Biol Chem. 2005 Feb 11;280(6):4722-9. [Content Brief]
[11]. Citterio E, et al. Biochemical and biological characterization of wild-type and ATPase-deficient Cockayne syndrome B repair protein. J Biol Chem. 1998 May 8;273(19):11844-51. [Content Brief]
[12]. Batenburg NL, et al. Cockayne syndrome group B protein regulates DNA double-strand break repair and checkpoint activation. EMBO J. 2015 May 12;34(10):1399-416. [Content Brief]
[13]. Batenburg NL, et al. ATM and CDK2 control chromatin remodeler CSB to inhibit RIF1 in DSB repair pathway choice. Nat Commun. 2017 Dec 4;8(1):1921. [Content Brief]
[14]. Weems JC, et al. Cockayne syndrome B protein regulates recruitment of the Elongin A ubiquitin ligase to sites of DNA damage. J Biol Chem. 2017 Apr 21;292(16):6431-6437. [Content Brief]
[15]. Ciaffardini F, et al. The cockayne syndrome B protein is essential for neuronal differentiation and neuritogenesis. Cell Death Dis. 2014 May 29;5(5):e1268. [Content Brief]
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