Slit2 Antibody (YA6786)
(Synonyms: Drad 1 antibody; E030015M03Rik antibody; E130320P19Rik antibody; FLJ14420 antibody; OTTHUMP00000158695 antibody; OTTHUMP00000217852 antibody; OTTHUMP00000217853 antibody; OTTHUMP00000217854 antibody; Slil 3 antibody; Slil3 antibody; Drad 1 antibody; E030015M03Rik antibody; E130320P19Rik antibody; FLJ14420 antibody; OTTHUMP00000158695 antibody; OTTHUMP00000217852 antibody; OTTHUMP00000217853 antibody; OTTHUMP00000217854 antibody; Slil 3 antibody; Slil3 antibody; Slit 2 antibody; Slit homolog 2 (Drosophila) antibody; Slit homolog 2 antibody; Slit homolog 2 protein antibody; Slit homolog 2 protein C-product antibody; Slit-2 antibody; Slit2 antibody; SLIT2_HUMAN antibody; )Slit2 Antibody (YA6786) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Slit2.
-
Host:
Rabbit
-
Isotype:
IgG
-
Application:
WB, ICC/IF, FC
-
Reactivity :
Human, Mouse, Rat
-
Formulation:
Supplied in 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
-
Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
|
ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
FC
FC: Flow Cytometry
|
|---|---|---|---|
| Dilution Ratio | 1:1000 | 1:100 | 1:50-1:1000 |
Product Details
Slit2 Antibody (YA6786) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Slit2.
-
Host Rabbit
-
Clonality Recombinant,Monoclonal
-
Species ReactivityHuman, Mouse, Rat
-
Observed Molecular WeightObserved band size: 50/200 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
-
Calculated Molecular Weight Predicted band size: 170 kDa
Recombinant protein within Human Slit2 aa 1,380-1,529 / 1,529.
affinity purified.
Non-conjugated
Unmodified
IgG
Product Properties
-
Appearance
Solution
-
Formulation
Supplied in 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
-
Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
-
Shipping
Shipping with blue ice.
Verification Images
-
Immunohistochemical analysis of paraffin-embedded human testis tissue using Cleaved PARP1 Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P87093, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam. -
Immunohistochemical analysis of paraffin-embedded mouse liver tissue using Cleaved PARP1 Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P87093, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam. -
Immunohistochemical analysis of paraffin-embedded rat stomach tissue using Cleaved PARP1 Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P87093, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam. -
Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue using Cleaved PARP1 Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P87093, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
Background
-
Function
Slit-2 (SLIT2) is a secreted ligand that binds ROBO receptors and functions as a repulsive axon-guidance cue[1]. Mechanistically, SLIT2/ROBO1 signaling regulates cytoskeletal remodeling, inhibits macrophage macropinocytosis, and limits NOD2-NF-κB-CXCL1 inflammatory signaling[2]. In immune models, Slit inhibited leukocyte chemotaxis, while Slit2 administration reduced neutrophil infiltration, tubular injury, and renal failure after renal ischemia-reperfusion injury[3][4]. In cancer models, SLIT2 biology is context-dependent: Slit-Robo signaling promoted tumor angiogenesis in melanoma, whereas SLIT2 inactivation was reported in lung and breast cancers[5][6]. Compared with related isoforms, SLIT1 is mainly nervous-system-associated, while SLIT2 and, to a lesser extent, SLIT3 are also detected in peripheral tissues[2]. For experimental applications, recombinant N-terminal SLIT2 (NSlit2) activates ROBO-dependent signaling, while ROBO1 neutralization or SLIT2-trap strategies block pathway activity in angiogenesis and glioblastoma models[2][5][7].
-
Subcellular Localization
Secreted
-
Expression
Tissue_Specificity: Fetal lung and kidney, and adult spinal cord. Weak expression in adult adrenal gland, thyroid, trachea and other tissues examined -
Isoforms & Post-Translational Modification
O94813 has three isomers: O94813-1: 169870 Da (predicted); O94813-2: 169395 Da (predicted); O94813-3: 168893 Da (predicted).
-
Subunit
Interacts with GREM1 (By similarity)
-
SwissProt ID
-
Synonyms
Drad 1 antibody; E030015M03Rik antibody; E130320P19Rik antibody; FLJ14420 antibody; OTTHUMP00000158695 antibody; OTTHUMP00000217852 antibody; OTTHUMP00000217853 antibody; OTTHUMP00000217854 antibody; Slil 3 antibody; Slil3 antibody; Drad 1 antibody; E030015M03Rik antibody; E130320P19Rik antibody; FLJ14420 antibody; OTTHUMP00000158695 antibody; OTTHUMP00000217852 antibody; OTTHUMP00000217853 antibody; OTTHUMP00000217854 antibody; Slil 3 antibody; Slil3 antibody; Slit 2 antibody; Slit homolog 2 (Drosophila) antibody; Slit homolog 2 antibody; Slit homolog 2 protein antibody; Slit homolog 2 protein C-product antibody; Slit-2 antibody; Slit2 antibody; SLIT2_HUMAN antibody;
Documentation
[1]. Brose K, et al. Slit proteins bind Robo receptors and have an evolutionarily conserved role in repulsive axon guidance. Cell. 1999 Mar 19;96(6):795-806. [Content Brief]
[2]. Bhosle VK, et al. SLIT2/ROBO1-signaling inhibits macropinocytosis by opposing cortical cytoskeletal remodeling. Nat Commun. 2020 Aug 17;11(1):4112. [Content Brief]
[3]. Wu JY, et al. The neuronal repellent Slit inhibits leukocyte chemotaxis induced by chemotactic factors. Nature. 2001 Apr 19;410(6831):948-52. [Content Brief]
[4]. Chaturvedi S, et al. Slit2 prevents neutrophil recruitment and renal ischemia-reperfusion injury. J Am Soc Nephrol. 2013 Jul;24(8):1274-87. [Content Brief]
[5]. Wang B, et al. Induction of tumor angiogenesis by Slit-Robo signaling and inhibition of cancer growth by blocking Robo activity. Cancer Cell. 2003 Jul;4(1):19-29. [Content Brief]
[6]. Dallol A, et al. SLIT2, a human homologue of the Drosophila Slit2 gene, has tumor suppressor activity and is frequently inactivated in lung and breast cancers. Cancer Res. 2002 Oct 15;62(20):5874-80. [Content Brief]
[7]. Geraldo LH, et al. SLIT2/ROBO signaling in tumor-associated microglia and macrophages drives glioblastoma immunosuppression and vascular dysmorphia. J Clin Invest. 2021 Aug 16;131(16):e141083. [Content Brief]
Powered by Bioz
