Slit2 Antibody (YA6786)

Cat. No.: HY-P87093: Data Sheet User Guide for Antibodies
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Slit2 Antibody (YA6786) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Slit2.

For research use only. We do not sell to patients.

Size	Stock	Quantity
20 μL	Get quote 2 - 3 weeks 1 - 2 Weeks 3 - 4 weeks 2 - 3 weeks	Estimated Time of Arrival: December 31
50 μL	Get quote 2 - 3 weeks 1 - 2 Weeks 3 - 4 weeks 2 - 3 weeks	Estimated Time of Arrival: December 31
100 μL	Get quote 2 - 3 weeks 1 - 2 Weeks 3 - 4 weeks 2 - 3 weeks	Estimated Time of Arrival: December 31
250 μL	Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

Slit2 Antibody (YA6786) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Slit2.

Host

Rabbit

Clonality

Recombinant,Monoclonal

Molecular Weight

Predicted band size: 170 kDa;

Observed band size: 50/200 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

O94813

Gene ID

9353 [NCBI]

Immunogen

Recombinant protein within Human Slit2 aa 1,380-1,529 / 1,529.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:1000
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:100
FC FC: Flow Cytometry	1:50-1:1000

Purity	affinity purified.	Conjugation	Non-conjugated
Modification	Unmodified	Isotype	IgG

Appearance

Liquid

Formulation

Supplied in 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Thought to act as molecular guidance cue in cellular migration, and function appears to be mediated by interaction with roundabout homolog receptors. During neural development involved in axonal navigation at the ventral midline of the neural tube and projection of axons to different regions. SLIT1 and SLIT2 seem to be essential for midline guidance in the forebrain by acting as repulsive signal preventing inappropriate midline crossing by axons projecting from the olfactory bulb. In spinal cord development may play a role in guiding commissural axons once they reached the floor plate by modulating the response to netrin. In vitro, silences the attractive effect of NTN1 but not its growth-stimulatory effect and silencing requires the formation of a ROBO1-DCC complex. May be implicated in spinal cord midline post-crossing axon repulsion. In vitro, only commissural axons that crossed the midline responded to SLIT2. In the developing visual system appears to function as repellent for retinal ganglion axons by providing a repulsion that directs these axons along their appropriate paths prior to, and after passage through, the optic chiasm. In vitro, collapses and repels retinal ganglion cell growth cones. Seems to play a role in branching and arborization of CNS sensory axons, and in neuronal cell migration. In vitro, Slit homolog 2 protein N-product, but not Slit homolog 2 protein C-product, repels olfactory bulb (OB) but not dorsal root ganglia (DRG) axons, induces OB growth cones collapse and induces branching of DRG axons. Seems to be involved in regulating leukocyte migration

Subcellular Localization：Secreted

Expression：
Tissue_Specificity: Fetal lung and kidney, and adult spinal cord. Weak expression in adult adrenal gland, thyroid, trachea and other tissues examined

Isoforms & Post-Translational Modification：O94813 has three isomers: O94813-1: 169870 Da (predicted); O94813-2: 169395 Da (predicted); O94813-3: 168893 Da (predicted).

Subunit：Interacts with GREM1 (By similarity)

Synonyms

Drad 1 antibody; E030015M03Rik antibody; E130320P19Rik antibody; FLJ14420 antibody; OTTHUMP00000158695 antibody; OTTHUMP00000217852 antibody; OTTHUMP00000217853 antibody; OTTHUMP00000217854 antibody; Slil 3 antibody; Slil3 antibody; Drad 1 antibody; E030015M03Rik antibody; E130320P19Rik antibody; FLJ14420 antibody; OTTHUMP00000158695 antibody; OTTHUMP00000217852 antibody; OTTHUMP00000217853 antibody; OTTHUMP00000217854 antibody; Slil 3 antibody; Slil3 antibody; Slit 2 antibody; Slit homolog 2 (Drosophila) antibody; Slit homolog 2 antibody; Slit homolog 2 protein antibody; Slit homolog 2 protein C-product antibody; Slit-2 antibody; Slit2 antibody; SLIT2_HUMAN antibody;

Documentation

User Guide for Antibodies (1077 KB)