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PRMT3 Antibody (YA1731)

(Synonyms: HRMT1L3)
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Based on 1 Customer Validation

PRMT3 Antibody (YA1731) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to PRMT3.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, IP

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Conjugation:
    Non-conjugated
ALL WB IHC-P
  • Experimental Validation Results for PRMT3 Antibody (YA1731)
  • Experimental Validation Results for PRMT3 Antibody (YA1731)
  • Experimental Validation Results for PRMT3 Antibody (YA1731)
  • PRMT3 Antibody (YA1731) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:500-1:1000 1:50-1:100 1:20

Product Details

Description

PRMT3 Antibody (YA1731) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to PRMT3.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 60 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 60 kDa
Species Reactivity Database

Entrez Gene: 10196 Human ;

SwissProt: O60678 Human ;

Immunogen

A synthetic peptide of human PRMT3 aa11-24.

Sensitivity

Endogenous

Purification

Affinity Purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3104107

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for PRMT3 Antibody (YA1731)
    Western blot analysis of extracts from C6(lane 2(20ug), NIH/3T3(lane 3(20ug) and Hela(lane 4(20ug) using PRMT3 Antibody (HY-P81986) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for PRMT3 Antibody (YA1731)
    Immunohistochemical analysis of paraffin-embedded Mouse brain tissue using PRMT3 Antibody (YA1731). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P81986, 1/100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for PRMT3 Antibody (YA1731)
    Immunohistochemical analysis of paraffin-embedded Mouse brain tissue using PRMT3 Antibody (YA1731). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P81986, 1/100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Background

  • Function

    PRMT3 (protein arginine methyltransferase 3) is a type I protein arginine methyltransferase that catalyzes asymmetric dimethylation of arginine residues and functions as a ribosomal protein methyltransferase involved in ribosome biogenesis and cellular protein synthesis regulation[1][2]. Mechanistically, PRMT3 is distinguished within the PRMT family by the presence of a zinc-finger domain that mediates substrate interaction, enabling regulation of diverse proteins involved in transcriptional control, post-transcriptional regulation, translation, and metabolic signaling pathways[3]. Through these activities, PRMT3 contributes to the control of gene expression and cellular metabolism, linking arginine methylation to fundamental biological processes[3]. In disease contexts, accumulating evidence indicates that PRMT3 participates in oncogenic programs by promoting metabolic reprogramming and gene expression changes associated with tumorigenesis[3]. Experimental studies further demonstrate that PRMT3 can influence cellular metabolism through regulation of glycolytic pathways and other metabolic processes relevant to cancer progression[3]. Compared with related PRMT isoforms, PRMT3 exhibits distinct substrate recognition, subcellular functions, and regulatory mechanisms, highlighting its nonredundant biological role[1][3]. For experimental applications, the allosteric inhibitor SGC707 provides a highly selective chemical probe for PRMT3, exhibiting nanomolar potency and substantial selectivity over other methyltransferases, thereby enabling mechanistic studies of PRMT3-dependent signaling and metabolic regulation[2]. Pharmacological inhibition of PRMT3 has also shown effects on hepatic triglyceride metabolism in preclinical models, supporting its utility for pathway interrogation and therapeutic target validation studies[4].

  • Subcellular Localization

    Cytoplasm, cytosol; Nucleus

  • Isoforms & Post-Translational Modification

    O60678 has 2 isomers: O60678-1: 59903 Da (predicted); O60678-2: 52902 Da (predicted).

  • Subunit

    Monomer and homodimer (By similarity). Interacts with EPB41L3 (via FERM domain); the interaction is direct and inhibits the protein-arginine N-methyltransferase activity of PRMT3 (PubMed:15334060).

  • SwissProt ID

    O60678

  • Gene ID
    10196 [NCBI]
  • Synonyms

    HRMT1L3

  • Research Field

    Epigenetics and Nuclear Signaling

[1]. Bachand F, et al. PRMT3 is a ribosomal protein methyltransferase that affects the cellular levels of ribosomal subunits. EMBO J. 2004 Jul 7;23(13):2641-50. [Content Brief]

[2]. Kaniskan HÜ, et al. Discovery of Potent and Selective Allosteric Inhibitors of Protein Arginine Methyltransferase 3 (PRMT3). J Med Chem. 2018 Feb 8;61(3):1204-1217. [Content Brief]

[3]. Hsu SH, et al. Protein arginine methyltransferase 3: A crucial regulator in metabolic reprogramming and gene expression in cancers. Cancer Lett. 2023 Feb 1;554:216008. [Content Brief]

[4]. de Jong LM, et al. PRMT3 inhibitor SGC707 reduces triglyceride levels and induces pruritus in Western-type diet-fed LDL receptor knockout mice. Sci Rep. 2022 Jan 10;12(1):483. [Content Brief]

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PRMT3 Antibody (YA1731) Related Classifications

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100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis of extracts from C6(lane 2(20ug), NIH/3T3(lane 3(20ug) and Hela(lane 4(20ug) using PRMT3 Antibody (HY-P81986) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded Mouse brain tissue using PRMT3 Antibody (YA1731). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P81986, 1/100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded Mouse brain tissue using PRMT3 Antibody (YA1731). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P81986, 1/100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • PRMT3 Antibody (YA1731) Product Image
    1/16
  • Experimental Validation Results for PRMT3 Antibody (YA1731)
  • Experimental Validation Results for PRMT3 Antibody (YA1731)
  • Experimental Validation Results for PRMT3 Antibody (YA1731)
  • PRMT3 Antibody (YA1731) Product Image
  • Experimental Validation Results for PRMT3 Antibody (YA1731)
    1/1
    MedchemExpress Validation
     Western blot analysis of extracts from C6(lane 2(20ug), NIH/3T3(lane 3(20ug) and Hela(lane 4(20ug) using PRMT3 Antibody (HY-P81986) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for PRMT3 Antibody (YA1731)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded Mouse brain tissue using PRMT3 Antibody (YA1731). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P81986, 1/100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for PRMT3 Antibody (YA1731)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded Mouse brain tissue using PRMT3 Antibody (YA1731). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P81986, 1/100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for PRMT3 Antibody (YA1731)
  • Experimental Validation Results for PRMT3 Antibody (YA1731)
  • Experimental Validation Results for PRMT3 Antibody (YA1731)
  • PRMT3 Antibody (YA1731) Product Image
    MedchemExpress Validation

    Western blot analysis of extracts from C6(lane 2(20ug), NIH/3T3(lane 3(20ug) and Hela(lane 4(20ug) using PRMT3 Antibody (HY-P81986) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • PRMT3 Antibody (YA1731) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded Mouse brain tissue using PRMT3 Antibody (YA1731). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P81986, 1/100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • PRMT3 Antibody (YA1731) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded Mouse brain tissue using PRMT3 Antibody (YA1731). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P81986, 1/100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • PRMT3 Antibody (YA1731) Product Image WB
  • PRMT3 Antibody (YA1731) Product Image IHC-P
  • PRMT3 Antibody (YA1731) Product Image IHC-P