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HPV16 E6 Antibody

Customer Review

Based on 1 Customer Validation

HPV16 E6 Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to HPV16 E6.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, IHC-F, FC, IF-Tissue

  • Reactivity :

    Virus

  • Formulation:

    Supplied in 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for HPV16 E6 Antibody
  • HPV16 E6 Antibody Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

Service
Data Download
Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
IHC-F Info
IHC-F: Immunohistochemistry-Frozen
IF-Tissue Info
IF-Tissue: Immunofluorescence-Tissue
FC Info
FC: Flow Cytometry
Dilution Ratio 1:500-1:2000 1:100-1:500 1:400-1:800 1:100-1:500 1ug/Test

Product Details

Description

HPV16 E6 Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to HPV16 E6.

  • Host Rabbit
  • Clonality Polyclonal
  • Species Reactivity
    Virus
  • Observed Molecular Weight
    Observed band size: 19 kD Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
Immunogen

Recombinant HPV16 E6

Purification

affinity purified

Conjugation

Non-conjugated

Isotype

IgG

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for HPV16 E6 Antibody
    Western blot analysis was performed by loading 0.5 μg (Lane 2), 1 μg (Lane 3), and 2 μg (Lane 4) of Protein E6, HPV16 (His) using HPV16 E6 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) was diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

Background

  • Function

    HPV16 E6 is a multifunctional nuclear phosphoprotein and a key oncoprotein in HPV16 carcinogenesis. HPV16 E6 belongs to the human papillomavirus early protein family. The C-terminal region of HPV16 E6 contains a conserved Cys-X-X-Cys motif, which can reversibly bind to Zn2+ and stabilize the protein structure. The N-terminus of HPV16 E6 has functional similarities with adenovirus E1A protein, facilitating interaction with cellular tumor suppressors. HPV16 E6 binds to the tumor suppressor protein p53 through E6-associated protein (E6AP), promotes its ubiquitination and proteasomal degradation, releases transcription factor E2F, activates the cell cycle and inhibits apoptosis. In addition, HPV16 E6 synergizes with E7 to immortalize primary human keratinocytes and neutralize E5-induced cytotoxicity. HPV16 E6 can also activate AP-1 and NF-κB signaling pathways, downregulate E-cadherin expression, enhance cell migration and invasion by destroying intercellular junctions, and interfere with cytoskeleton rearrangement to promote epithelial-mesenchymal transition. The upstream regulatory factors of HPV16 E6 include viral promoters (such as LCR); downstream targets include p53, E2F, p16 and E-cadherin, which are involved in cell cycle control and adhesion regulation.

HPV16 E6 Antibody Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis was performed by loading 0.5 μg (Lane 2), 1 μg (Lane 3), and 2 μg (Lane 4) of Protein E6, HPV16 (His) using HPV16 E6 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) was diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • HPV16 E6 Antibody Product Image
    1/16
  • Experimental Validation Results for HPV16 E6 Antibody
  • HPV16 E6 Antibody Product Image
  • Experimental Validation Results for HPV16 E6 Antibody
    1/1
    MedChemExpress Validation
     Western blot analysis was performed by loading 0.5 μg (Lane 2), 1 μg (Lane 3), and 2 μg (Lane 4) of Protein E6, HPV16 (His) using HPV16 E6 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) was diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).
  • Experimental Validation Results for HPV16 E6 Antibody
  • HPV16 E6 Antibody Product Image
    MedChemExpress Validation

    Western blot analysis was performed by loading 0.5 μg (Lane 2), 1 μg (Lane 3), and 2 μg (Lane 4) of Protein E6, HPV16 (His) using HPV16 E6 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) was diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • HPV16 E6 Antibody Product Image WB