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HMGCS1 Antibody (YA6977)

(Synonyms: 3 hydroxy 3 methylglutaryl Coenzyme A synthase 1 (soluble) antibody; 3-hydroxy-3-methylglutaryl coenzyme A synthase antibody; cytoplasmic antibody; EC 2.3.3.10 antibody; HMCS1_HUMAN antibody; HMG CoA synthase antibody; HMG-CoA synthase antibody; HMGCS antibody; HMGCS1 antibody; Hydroxymethylglutaryl CoA synthase, cytoplasmic antibody)
Customer Review

Based on 1 Customer Validation

HMGCS1 Antibody (YA6977) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to HMGCS1.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IP

  • Reactivity :

    Human, Mouse, Rat, Monkey

  • Formulation:

    Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for HMGCS1 Antibody (YA6977)
  • Experimental Validation Results for HMGCS1 Antibody (YA6977)
  • HMGCS1 Antibody (YA6977) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
Dilution Ratio 1:2000

Product Details

Description

HMGCS1 Antibody (YA6977) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to HMGCS1.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat, Monkey
  • Observed Molecular Weight
    Observed band size: 55 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 57 kDa
Immunogen

Recombinant protein within human HMGCS1 aa 421-520.

Sensitivity

Endogenous

Purification

Protein A affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for HMGCS1 Antibody (YA6977)
    Western blot analysis of extracts from HepG2 (lane 1(20μg)) 、A549 (lane 2(20μg)) 、Mouse liver (lane 3(20μg)) and Rat liver (lane 4(20μg)) using HMGCS1 Antibody (HY-P87294) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/2000) and Loading control antibody (Hsp90, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for HMGCS1 Antibody (YA6977)
    Western blot analysis of extracts from NIH-3T3 (lane 1(20μg)) and C2C12 (lane 2(20μg)) using HMGCS1 Antibody (HY-P87294) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/2000) and Loading control antibody (Hsp90, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

Background

  • Function

    HMGCS1 catalyzes the condensation of acetyl-CoA with acetoacetyl-CoA to form HMG-CoA, which is converted by HMG-CoA reductase (HMGCR) into mevalonate, a precursor for cholesterol synthesis

  • Subcellular Localization

    Cytoplasm

  • Isoforms & Post-Translational Modification

    Q01581: 520 amino acids, molecular weight 57294 Da.

  • Subunit

    Homodimer

  • SwissProt ID

    Q01581

  • Synonyms

    3 hydroxy 3 methylglutaryl Coenzyme A synthase 1 (soluble) antibody; 3-hydroxy-3-methylglutaryl coenzyme A synthase antibody; cytoplasmic antibody; EC 2.3.3.10 antibody; HMCS1_HUMAN antibody; HMG CoA synthase antibody; HMG-CoA synthase antibody; HMGCS antibody; HMGCS1 antibody; Hydroxymethylglutaryl CoA synthase, cytoplasmic antibody

HMGCS1 Antibody (YA6977) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from HepG2 (lane 1(20μg)) 、A549 (lane 2(20μg)) 、Mouse liver (lane 3(20μg)) and Rat liver (lane 4(20μg)) using HMGCS1 Antibody (HY-P87294) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/2000) and Loading control antibody (Hsp90, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from NIH-3T3 (lane 1(20μg)) and C2C12 (lane 2(20μg)) using HMGCS1 Antibody (HY-P87294) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/2000) and Loading control antibody (Hsp90, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

  • HMGCS1 Antibody (YA6977) Product Image
    1/16
  • Experimental Validation Results for HMGCS1 Antibody (YA6977)
  • Experimental Validation Results for HMGCS1 Antibody (YA6977)
  • HMGCS1 Antibody (YA6977) Product Image
  • Experimental Validation Results for HMGCS1 Antibody (YA6977)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from HepG2 (lane 1(20μg)) 、A549 (lane 2(20μg)) 、Mouse liver (lane 3(20μg)) and Rat liver (lane 4(20μg)) using HMGCS1 Antibody (HY-P87294) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/2000) and Loading control antibody (Hsp90, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for HMGCS1 Antibody (YA6977)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from NIH-3T3 (lane 1(20μg)) and C2C12 (lane 2(20μg)) using HMGCS1 Antibody (HY-P87294) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/2000) and Loading control antibody (Hsp90, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for HMGCS1 Antibody (YA6977)
  • Experimental Validation Results for HMGCS1 Antibody (YA6977)
  • HMGCS1 Antibody (YA6977) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from HepG2 (lane 1(20μg)) 、A549 (lane 2(20μg)) 、Mouse liver (lane 3(20μg)) and Rat liver (lane 4(20μg)) using HMGCS1 Antibody (HY-P87294) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/2000) and Loading control antibody (Hsp90, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

  • HMGCS1 Antibody (YA6977) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from NIH-3T3 (lane 1(20μg)) and C2C12 (lane 2(20μg)) using HMGCS1 Antibody (HY-P87294) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/2000) and Loading control antibody (Hsp90, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

  • HMGCS1 Antibody (YA6977) Product Image WB
  • HMGCS1 Antibody (YA6977) Product Image WB