SLC39A6 Antibody
(Synonyms: SLC39A6, Estrogen-regulated protein LIV-1, Zinc transporter ZIP6, LIV1, ZIP6)SLC39A6 Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to SLC39A6.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, IHC-P, ICC/IF, ELISA
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Formulation:
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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IHC-P
IHC-P: Immunohistochemistry-Paraffin
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ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
ELISA
ELISA: Enzyme Linked Immunosorbent Assay
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|---|---|---|---|---|
| Dilution Ratio | 1:500-2000 | 1:50-200 | 1:50-200 | 1:2000-10000 |
Product Details
SLC39A6 Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to SLC39A6.
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Host Rabbit
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Clonality polyclonal
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Calculated Molecular Weight Predicted band size: 85 kDa;
Recombinant protein of Human SLC39A6.
Endogenous
affinity purified.
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
SLC39A6 is a Zinc-influx transporter which plays a role in zinc homeostasis and in the induction of epithelial-to-mesenchymal transition (EMT). When associated with SLC39A10, the heterodimer formed by SLC39A10 and SLC39A6 mediates cellular zinc uptake to trigger cells to undergo epithelial- to-mesenchymal transition (EMT). The SLC39A10-SLC39A6 heterodimer also controls NCAM1 phosphorylation and its integration into focal adhesion complexes during EMT (By similarity). Zinc influx inactivates GSK3B, enabling unphosphorylated SNAI1 in the nucleus to down-regulate adherence genes such as CDH1, causing loss of cell adherence. In addition, the SLC39A10-SLC39A6 heterodimer plays an essential role in initiating mitosis by importing zinc into cells to initiate a pathway resulting in the onset of mitosis. Participates in the T-cell receptor signaling regulation by mediating cellular zinc uptake into activated lymphocytes. Regulates the zinc influx necessary for proper meiotic progression to metaphase II (MII) that allows the oocyte-to-egg transition[1][2][3][4][5][6][7][8][9].
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Subcellular Localization
Cell membrane; Cell projection, lamellipodium membrane; Membrane raft; Apical cell membrane
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Expression
Tissue_Specificity: Highly expressed in the breast, prostate, placenta, kidney, pituitary and corpus callosum. Weakly expressed in heart and intestine. Also highly expressed in cells derived from an adenocarcinoma of the cervix and lung carcinoma.
Induction: Up-regulated by estrogen in breast cancer cells lines. Decreased protein level in response to zinc treatment. Increased upon T cell activation. Induced by STAT3. -
Isoforms & Post-Translational Modification
SLC39A6 has 2 isoforms, Q13433-1: amino acid length is 755, molecular weight is 85047 Da (predicted); Q13433-2: amino acid length is 433, molecular weight is 48605 Da (predicted).Cleaved on the N-terminus before locating to the plasma membrane
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Subunit
Interacts with SLC39A10; which triggers cells to undergo EMT and mitosis.
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SwissProt ID
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Synonyms
SLC39A6, Estrogen-regulated protein LIV-1, Zinc transporter ZIP6, LIV1, ZIP6
Documentation
[1]. Taylor KM, et al. Structure-function analysis of LIV-1, the breast cancer-associated protein that belongs to a new subfamily of zinc transporters. Biochem J. 2003 Oct 1;375(Pt 1):51-9. [Content Brief]
[2]. Chowanadisai W, et al. Zip6 (LIV-1) regulates zinc uptake in neuroblastoma cells under resting but not depolarizing conditions. Brain Res. 2008 Mar 14;1199:10-9. [Content Brief]
[3]. Yu M, et al. Regulation of T cell receptor signaling by activation-induced zinc influx. J Exp Med. 2011 Apr 11;208(4):775-85. [Content Brief]
[4]. Hogstrand C, et al. A mechanism for epithelial-mesenchymal transition and anoikis resistance in breast cancer triggered by zinc channel ZIP6 and STAT3 (signal transducer and activator of transcription 3). Biochem J. 2013 Oct 15;455(2):229-37. [Content Brief]
[5]. Taylor KM, et al. Zinc transporter ZIP10 forms a heteromer with ZIP6 which regulates embryonic development and cell migration. Biochem J. 2016 Aug 15;473(16):2531-44. [Content Brief]
[6]. Kim B, et al. Zap70 Regulates TCR-Mediated Zip6 Activation at the Immunological Synapse. Front Immunol. 2021;12:687367. [Content Brief]
[7]. Nimmanon T, et al. The ZIP6/ZIP10 heteromer is essential for the zinc-mediated trigger of mitosis. Cell Mol Life Sci. 2021 Feb;78(4):1781-1798. [Content Brief]
[8]. Colomar-Carando N, et al. Zip6 Transporter Is an Essential Component of the Lymphocyte Activation Machinery. J Immunol. 2019 Jan 15;202(2):441-450. [Content Brief]
[9]. Kong BY, et al. Maternally-derived zinc transporters ZIP6 and ZIP10 drive the mammalian oocyte-to-egg transition. Mol Hum Reprod. 2014 Nov;20(11):1077-89. [Content Brief]
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