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TRIM25 Antibody (YA2278)

(Synonyms: TRIM25; EFP; RNF147; ZNF147; E3 ubiquitin/ISG15 ligase TRIM25; Estrogen-responsive finger protein; RING finger protein 147; Tripartite motif-containing protein 25; Ubiquitin/ISG15-conjugating enzyme TRIM25; Zinc finger protein 147)
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Based on 1 Customer Validation

TRIM25 Antibody (YA2278) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to TRIM25.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, ICC/IF, IP

  • Reactivity :

    Human, Rat

  • Formulation:

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for TRIM25 Antibody (YA2278)
  • TRIM25 Antibody (YA2278) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:500-1:1000 1:50-1:200 1:20

Product Details

Description

TRIM25 Antibody (YA2278) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to TRIM25.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Rat
  • Observed Molecular Weight
    Observed band size: 71 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 71 kDa
Species Reactivity Database
Immunogen

A synthetic peptide of human TRIM25

Sensitivity

Endogenous

Purification

Affinity Purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3104638

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for TRIM25 Antibody (YA2278)
    Western blot analysis was performed on extracts from HeLa (lane 1, 15 μg), K562 (lane 2, 15 μg), MCF-7 (lane 3, 15 μg), 293 (lane 4, 15 μg), and HepG2 (lane 5, 15 μg) using TRIM25 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

Background

  • Function

    TRIM25 functions as a ubiquitin E3 ligase and as an ISG15 E3 ligase. Involved in innate immune defense against viruses by mediating ubiquitination of RIGI and IFIH1. Mediates 'Lys-63'-linked polyubiquitination of the RIGI N-terminal CARD-like region and may play a role in signal transduction that leads to the production of interferons in response to viral infection. Mediates 'Lys-63'-linked polyubiquitination of IFIH1. Promotes ISGylation of 14-3-3 sigma (SFN), an adapter protein implicated in the regulation of a large spectrum signaling pathway. Mediates estrogen action in various target organs. Mediates the ubiquitination and subsequent proteasomal degradation of ZFHX3. Plays a role in promoting the restart of stalled replication forks via interaction with the KHDC3L-OOEP scaffold and subsequent ubiquitination of BLM, resulting in the recruitment and retainment of BLM at DNA replication forks. Plays an essential role in the antiviral activity of ZAP/ZC3HAV1; an antiviral protein which inhibits the replication of certain viruses. Mechanistically, mediates 'Lys-63'-linked polyubiquitination of ZAP/ZC3HAV1 that is required for its optimal binding to target mRNA. Also mediates the ubiquitination of various substrates implicated in stress granule formation, nonsense-mediated mRNA decay, nucleoside synthesis and mRNA translation and stability[1][2][3][4][5][6][7][8][9][10][11][12][13].

  • Subcellular Localization

    Cytoplasm; Cytoplasm, Stress granule; Nucleus

  • Expression


    Tissue_specificity:It is expressed (at the protein level) in breast tumors. It is ubiquitous.

    Induction:By interferons

  • Subunit

    Forms homodimers (PubMed:27154206, PubMed:27425606, PubMed:29357390, PubMed:31710640). Interacts (via SPRY domain) with RIGI (via CARD domain). Interacts with ZFHX3. Interacts with NLRP12; this interaction reduces the E3 ubiquitin ligase TRIM25-mediated 'Lys-63'-linked RIGI activation. Interacts with the KHDC3L/FILIA-OOEP/FLOPED scaffold complex and BLM at DNA replication forks (By similarity). Interacts with RTN3; this interaction prevents RIGI ubiquitination (PubMed:34313226). Interacts with YWHAE (PubMed:22607805)

  • SwissProt ID

    Q14258

  • Gene ID
    7706 [NCBI]
  • Synonyms

    TRIM25; EFP; RNF147; ZNF147; E3 ubiquitin/ISG15 ligase TRIM25; Estrogen-responsive finger protein; RING finger protein 147; Tripartite motif-containing protein 25; Ubiquitin/ISG15-conjugating enzyme TRIM25; Zinc finger protein 147

  • Research Field

    Cell Biology

[1]. Zou W, et al. The interferon-inducible ubiquitin-protein isopeptide ligase (E3) EFP also functions as an ISG15 E3 ligase. J Biol Chem. 2006 Feb 17;281(7):3989-94. [Content Brief]

[2]. Gack MU, et al. TRIM25 RING-finger E3 ubiquitin ligase is essential for RIG-I-mediated antiviral activity. Nature. 2007 Apr 19;446(7138):916-920. [Content Brief]

[3]. Gupta S, et al. Herpesvirus deconjugases inhibit the IFN response by promoting TRIM25 autoubiquitination and functional inactivation of the RIG-I signalosome. PLoS Pathog. 2018 Jan;14(1):e1006852. [Content Brief]

[4]. Lian H, et al. The Zinc-Finger Protein ZCCHC3 Binds RNA and Facilitates Viral RNA Sensing and Activation of the RIG-I-like Receptors. Immunity. 2018 Sep 18;49(3):438-448.e5. [Content Brief]

[5]. Gupta S, et al. 14-3-3 scaffold proteins mediate the inactivation of trim25 and inhibition of the type I interferon response by herpesvirus deconjugases. PLoS Pathog. 2019 Nov;15(11):e1008146. [Content Brief]

[6]. Chiang C, et al. The Small t Antigen of JC Virus Antagonizes RIG-I-Mediated Innate Immunity by Inhibiting TRIM25's RNA Binding Ability. mBio. 2021 Apr 13;12(2):. [Content Brief]

[7]. Tanaka Y, et al. Human metapneumovirus M2-2 protein inhibits RIG-I signaling by preventing TRIM25-mediated RIG-I ubiquitination. Front Immunol. 2022;13:970750. [Content Brief]

[8]. Oshiumi H, et al. A distinct role of Riplet-mediated K63-Linked polyubiquitination of the RIG-I repressor domain in human antiviral innate immune responses. PLoS Pathog. 2013;9(8):e1003533. [Content Brief]

[9]. Nakasato N, et al. A ubiquitin E3 ligase Efp is up-regulated by interferons and conjugated with ISG15. Biochem Biophys Res Commun. 2006 Dec 15;351(2):540-6. [Content Brief]

[10]. Dong XY, et al. Oestrogen causes ATBF1 protein degradation through the oestrogen-responsive E3 ubiquitin ligase EFP. Biochem J. 2012 Jun 15;444(3):581-90. [Content Brief]

[11]. Li MM, et al. TRIM25 Enhances the Antiviral Action of Zinc-Finger Antiviral Protein (ZAP). PLoS Pathog. 2017 Jan;13(1):e1006145. [Content Brief]

[12]. Zheng X, et al. TRIM25 Is Required for the Antiviral Activity of Zinc Finger Antiviral Protein. J Virol. 2017 May 1;91(9):. [Content Brief]

[13]. Yang E, et al. Elucidation of TRIM25 ubiquitination targets involved in diverse cellular and antiviral processes. PLoS Pathog. 2022 Sep;18(9):e1010743. [Content Brief]

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TRIM25 Antibody (YA2278) Related Classifications

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100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis was performed on extracts from HeLa (lane 1, 15 μg), K562 (lane 2, 15 μg), MCF-7 (lane 3, 15 μg), 293 (lane 4, 15 μg), and HepG2 (lane 5, 15 μg) using TRIM25 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • TRIM25 Antibody (YA2278) Product Image
    1/16
  • Experimental Validation Results for TRIM25 Antibody (YA2278)
  • TRIM25 Antibody (YA2278) Product Image
  • Experimental Validation Results for TRIM25 Antibody (YA2278)
    1/1
    MedchemExpress Validation
     Western blot analysis was performed on extracts from HeLa (lane 1, 15 μg), K562 (lane 2, 15 μg), MCF-7 (lane 3, 15 μg), 293 (lane 4, 15 μg), and HepG2 (lane 5, 15 μg) using TRIM25 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for TRIM25 Antibody (YA2278)
  • TRIM25 Antibody (YA2278) Product Image
    MedchemExpress Validation

    Western blot analysis was performed on extracts from HeLa (lane 1, 15 μg), K562 (lane 2, 15 μg), MCF-7 (lane 3, 15 μg), 293 (lane 4, 15 μg), and HepG2 (lane 5, 15 μg) using TRIM25 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • TRIM25 Antibody (YA2278) Product Image WB