MBNL1 Antibody (YA9767)
(Synonyms: Muscleblind Like Splicing Regulator 1, EXP, KIAA0428, MBNL, Triplet-Expansion RNA-Binding Protein, Muscleblind-Like Protein 1, EXP42, EXP40, EXP35, Muscleblind-Like Splicing Regulator 1, Muscleblind (Drosophila)-Like, Muscleblind-Like (Drosophila), Muscleblind-Like)MBNL1 Antibody (YA9767) is a Rabbit-derived and non-conjugated IgG Monoclonal, Recombinant antibody, targeting to MBNL1.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, FC, ICC/IF
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Reactivity :
Human, mouse
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Formulation:
Supplied in PBS (pH 7.4) containing 50% glycerol, 0.05% BSA and 0.02% sodium azide.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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FC
FC: Flow Cytometry
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ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
|---|---|---|---|
| Dilution Ratio | 1:1000-5000 | 1:200-2000 | 1:100-1000 |
Product Details
MBNL1 Antibody (YA9767) is a Rabbit-derived and non-conjugated IgG Monoclonal, Recombinant antibody, targeting to MBNL1.
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Host Rabbit
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Species ReactivityHuman, mouse
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Observed Molecular WeightObserved band size: 40 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 42 kDa
A synthesized peptide derived from human MBNL1
affinity purified.
Non-conjugated
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in PBS (pH 7.4) containing 50% glycerol, 0.05% BSA and 0.02% sodium azide.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
MBNL1 mediates pre-mRNA alternative splicing regulation. Acts either as activator or repressor of splicing on specific pre-mRNA targets. Inhibits cardiac troponin-T (TNNT2) pre-mRNA exon inclusion but induces insulin receptor (IR) pre-mRNA exon inclusion in muscle. Antagonizes the alternative splicing activity pattern of CELF proteins. Regulates the TNNT2 exon 5 skipping through competition with U2AF2. Inhibits the formation of the spliceosome A complex on intron 4 of TNNT2 pre-mRNA. Binds to the stem-loop structure within the polypyrimidine tract of TNNT2 intron 4 during spliceosome assembly. Binds to the 5'-YGCU(U/G)Y-3'consensus sequence. Binds to the IR RNA. Binds to expanded CUG repeat RNA, which folds into a hairpin structure containing GC base pairs and bulged, unpaired U residues. Together with RNA binding proteins RBPMS and RBFOX2, activates vascular smooth muscle cells alternative splicing events. Regulates NCOR2 alternative splicing (By similarity)[1].
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Subcellular Localization
Nucleus; Cytoplasm; Cytoplasmic granule
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Expression
Tissue_Specificity: Highly expressed in cardiac, skeletal muscle and during myoblast differentiation. Weakly expressed in other tissues (at protein level). Expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. -
Isoforms & Post-Translational Modification
MBNL1 has 7 isoforms, Q9NR56-1: amino acid length is 388, molecular weight is 41817 Da (predicted); Q9NR56-2: amino acid length is 370, molecular weight is 39831 Da (predicted); Q9NR56-3: amino acid length is 302, molecular weight is 33050 Da (predicted); Q9NR56-4: amino acid length is 314, molecular weight is 34211 Da (predicted); Q9NR56-5: amino acid length is 382, molecular weight is 40992 Da (predicted); Q9NR56-6: amino acid length is 342, molecular weight is 37074 Da (predicted); Q9NR56-7: amino acid length is 340, molecular weight is 36992 Da (predicted).
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Subunit
Interacts with DDX1 and YBX1.
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SwissProt ID
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Synonyms
Muscleblind Like Splicing Regulator 1, EXP, KIAA0428, MBNL, Triplet-Expansion RNA-Binding Protein, Muscleblind-Like Protein 1, EXP42, EXP40, EXP35, Muscleblind-Like Splicing Regulator 1, Muscleblind (Drosophila)-Like, Muscleblind-Like (Drosophila), Muscleblind-Like
Documentation
References
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