Pyruvate Dehydrogenase E1 beta Antibody (YA2374)

Cat. No.: HY-P82629: Data Sheet COA
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User Guide for Antibodies
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Pyruvate Dehydrogenase E1 beta Antibody (YA2374) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Pyruvate Dehydrogenase E1 beta.

For research use only. We do not sell to patients.

Size	Stock	Quantity
20 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
250 μL	Get quote

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Pyruvate Dehydrogenase E1 beta Antibody (YA2374) Featured Recommendations:

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation
References

Description

Pyruvate Dehydrogenase E1 beta Antibody (YA2374) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Pyruvate Dehydrogenase E1 beta.

Host

Rabbit

Clonality

Recombinant,Monoclonal

Molecular Weight

Predicted band size: 39 kDa;

Observed band size: 35 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

P11177

Gene ID

5162 [NCBI]

Immunogen

A synthesized peptide derived from human Pyruvate Dehydrogenase E1 beta subunit aa300-359/359.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:1000-1:2000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:100-1:200
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:50-1:200
FC FC: Flow Cytometry	1:50-1:100
IP IP: Immunoprecipitation	1:50

Sensitivity	Endogenous	Purity	Affinity Chromatography
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in rabbit IgG in 10mM PBS, pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2), and thereby links the glycolytic pathway to the tricarboxylic cycle

Subcellular Localization：Mitochondrion matrix

Isoforms & Post-Translational Modification：P11177 has 3 isomers: P11177-1: 39233 Da (predicted); P11177-2: 37200 Da (predicted); P11177-3: 37514 Da (predicted).

Subunit：Heterotetramer of two PDHA1 and two PDHB subunits (PubMed:12651851, PubMed:17474719, PubMed:19081061, PubMed:29970614). The heterotetramer interacts with DLAT, and is part of the multimeric pyruvate dehydrogenase complex that contains multiple copies of pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (DLAT, E2) and lipoamide dehydrogenase (DLD, E3) (PubMed:14638692). These subunits are bound to an inner core composed of about 48 DLAT and 12 PDHX molecules (PubMed:14638692). Interacts with DLAT (PubMed:20160912)

Database

Entrez Gene: 5162 Human ; 68263 Mouse ; 289950 Rat

SwissProt: P11177 Human ; Q9D051 Mouse ; P49432 Rat

OMIM: 614111 Human

Research Field

Signal Transduction

Synonyms

pdhB; DHBD; PDHE1 B; PDHE1-B; PHE1B

Documentation

Select Batch:

Data Sheet (234 KB) SDS (251 KB)

COA (205 KB)

User Guide for Antibodies (1077 KB)

References

[1]. /biology-dictionary/hepg2-cell.html [Content Brief]

Pyruvate Dehydrogenase E1 beta Antibody (YA2374) Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Pyruvate Dehydrogenase E1 beta Antibody (YA2374)

Pyruvate Dehydrogenase E1 beta Antibody (YA2374) Featured Recommendations:

Product Detail

Background

Documentation

References

Pyruvate Dehydrogenase E1 beta Antibody (YA2374) Related Classifications

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