VPS18 Antibody (YA2445)
(Synonyms: hVPS18; PEP3; vps18)
Based on 1 Customer Validation
VPS18 Antibody (YA2445) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to VPS18.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, ICC/IF, FC
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Reactivity :
Human, Mouse, Rat
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Formulation:
Supplied in rabbit IgG in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
|
ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
FC
FC: Flow Cytometry
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|---|---|---|---|
| Dilution Ratio | 1:1000-1:2000 | 1:50-1:200 | 1:50-1:100 |
Product Details
VPS18 Antibody (YA2445) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to VPS18.
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Host Rabbit
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Clonality Recombinant,Monoclonal
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Species ReactivityHuman, Mouse, Rat
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Observed Molecular WeightObserved band size: 100 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 110 kDa
Entrez Gene: 57617 Human ; 228545 Mouse ; 296083 Rat
SwissProt: Q9P253 Human ; Q8R307 Mouse ;
OMIM: 608551 Human
A synthesized peptide derived from human VPS18 aa1-28.
Endogenous
Affinity Purified
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in rabbit IgG in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.
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Concentration
Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
VPS18 plays a role in vesicle-mediated protein trafficking to lysosomal compartments including the endocytic membrane transport and autophagic pathways. Believed to act as a core component of the putative HOPS and CORVET endosomal tethering complexes which are proposed to be involved in the Rab5-to-Rab7 endosome conversion probably implicating MON1A/B, and via binding SNAREs and SNARE complexes to mediate tethering and docking events during SNARE-mediated membrane fusion. The HOPS complex is proposed to be recruited to Rab7 on the late endosomal membrane and to regulate late endocytic, phagocytic and autophagic traffic towards lysosomes. The CORVET complex is proposed to function as a Rab5 effector to mediate early endosome fusion probably in specific endosome subpopulations. Required for fusion of endosomes and autophagosomes with lysosomes. Involved in dendrite development of Pukinje cells[1][2][3][4].
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Subcellular Localization
Late endosome membrane; Peripheral membrane protein; Cytoplasmic side; Lysosome membrane; Peripheral membrane protein; Cytoplasmic side; Early endosome; Cytoplasmic vesicle, autophagosome; Cytoplasmic vesicle, clathrin-coated vesicle
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Expression
Tissue_specificity:Ubiquitous. Expression was highest in heart and low in lung -
Subunit
Core component of at least two putative endosomal tethering complexes, the homotypic fusion and vacuole protein sorting (HOPS) complex and the class C core vacuole/endosome tethering (CORVET) complex.
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SwissProt ID
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Synonyms
hVPS18; PEP3; vps18
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Research Field
Signal Transduction
Documentation
References
[1]. Kim BY, et al. Molecular characterization of mammalian homologues of class C Vps proteins that interact with syntaxin-7. J Biol Chem. 2001 Aug 3;276(31):29393-402. [Content Brief]
[2]. Solinger JA, et al. Tethering complexes in the endocytic pathway: CORVET and HOPS. FEBS J. 2013 Jun;280(12):2743-57. [Content Brief]
[3]. Jiang P, et al. The HOPS complex mediates autophagosome-lysosome fusion through interaction with syntaxin 17. Mol Biol Cell. 2014 Apr;25(8):1327-37. [Content Brief]
[4]. Wartosch L, et al. Recruitment of VPS33A to HOPS by VPS16 Is Required for Lysosome Fusion with Endosomes and Autophagosomes. Traffic. 2015 Jul;16(7):727-42. [Content Brief]
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