APPBP1 Antibody (YA2131)(PBS only)

Cat. No.: HY-P82386A: Data Sheet User Guide for Antibodies
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APPBP1 Antibody (YA2131) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to APPBP1.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation
References

Description

APPBP1 Antibody (YA2131) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to APPBP1.

Host

Rabbit

Molecular Weight

Predicted band size: 60 kDa;

Observed band size: 60 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

Q13564

Gene ID

8883 [NCBI]

Immunogen

A synthetic peptide of human APPBP1

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000
IP IP: Immunoprecipitation	1:20

Purity	Affinity Purified	Conjugation	Non-conjugated
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Regulatory subunit of the dimeric UBA3-NAE1 E1 enzyme. E1 activates NEDD8 by first adenylating its C-terminal glycine residue with ATP, thereafter linking this residue to the side chain of the catalytic cysteine, yielding a NEDD8-UBA3 thioester and free AMP. E1 finally transfers NEDD8 to the catalytic cysteine of UBE2M. Necessary for cell cycle progression through the S-M checkpoint. Overexpression of NAE1 causes apoptosis through deregulation of NEDD8 conjugation. The covalent attachment of NEDD8 to target proteins is known as 'neddylation' and the process is involved in the regulation of cell growth, viability and development

Subcellular Localization：Cell membrane

Expression：
Tissue_specificity:Ubiquitous in fetal tissues. Expressed throughout the human brain

Isoforms & Post-Translational Modification：Q13564 has 4 isomers: Q13564-1: 60246 Da (predicted); Q13564-2: 59420 Da (predicted); Q13564-3: 50624 Da (predicted); Q13564-4: 60517 Da (predicted).
Ubiquitinated by TRIP12, leading to its degradation by the proteasome

Subunit：Heterodimer of UBA3 and NAE1. The complex binds NEDD8 and UBE2M. Binds APP and TP53BP2

Research Field

Cell Biology

Synonyms

NEDD8 activating enzyme E1 subunit 1; HPP1; ula-1; APPBP1; A-116A10.1

Documentation

User Guide for Antibodies (1077 KB)

References

[1]. /biology-dictionary/scc-25-cell.html [Content Brief]