APPBP1 Antibody (YA2131)

Cat. No.: HY-P82386: Data Sheet COA
SDS
User Guide for Antibodies
FAQs
Technical Support

APPBP1 Antibody (YA2131) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to APPBP1.

For research use only. We do not sell to patients.

Size	Stock	Quantity
Free Sample	Apply now
10 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
250 μL	Get quote

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APPBP1 Antibody (YA2131) Featured Recommendations:

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
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Background
Documentation

Description

APPBP1 Antibody (YA2131) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to APPBP1.

Host

Rabbit

Clonality

Recombinant,Monoclonal

Molecular Weight

Predicted band size: 60 kDa;

Observed band size: 60 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

Q13564

Gene ID

8883 [NCBI]

Immunogen

A synthetic peptide of human APPBP1

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000
IP IP: Immunoprecipitation	1:20

Sensitivity	Endogenous	Purity	Affinity Purified
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Verification Image

Western blot analysis of extracts from Hela(lane 2(20ug) , A431(lane 3(20ug) and Ramos(lane 4(20ug) using APPBP1 Antibody (HY-P82386) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

Background

Function：Regulatory subunit of the dimeric UBA3-NAE1 E1 enzyme. E1 activates NEDD8 by first adenylating its C-terminal glycine residue with ATP, thereafter linking this residue to the side chain of the catalytic cysteine, yielding a NEDD8-UBA3 thioester and free AMP. E1 finally transfers NEDD8 to the catalytic cysteine of UBE2M. Necessary for cell cycle progression through the S-M checkpoint. Overexpression of NAE1 causes apoptosis through deregulation of NEDD8 conjugation. The covalent attachment of NEDD8 to target proteins is known as 'neddylation' and the process is involved in the regulation of cell growth, viability and development

Subcellular Localization：Cell membrane

Expression：
Tissue_specificity:Ubiquitous in fetal tissues. Expressed throughout the human brain

Isoforms & Post-Translational Modification：Q13564 has 4 isomers: Q13564-1: 60246 Da (predicted); Q13564-2: 59420 Da (predicted); Q13564-3: 50624 Da (predicted); Q13564-4: 60517 Da (predicted).
Ubiquitinated by TRIP12, leading to its degradation by the proteasome

Subunit：Heterodimer of UBA3 and NAE1. The complex binds NEDD8 and UBE2M. Binds APP and TP53BP2

RRID

AB_3104495

Database

Entrez Gene: 8883 Human

SwissProt: Q13564 Human

OMIM: 620210 Human

Research Field

Cell Biology

Synonyms

NEDD8 activating enzyme E1 subunit 1; HPP1; ula-1; APPBP1; A-116A10.1

Documentation

Select Batch:

Data Sheet (234 KB) SDS (251 KB)

COA (205 KB)

User Guide for Antibodies (1077 KB)

APPBP1 Antibody (YA2131) Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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APPBP1 Antibody (YA2131)

APPBP1 Antibody (YA2131) Featured Recommendations:

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Background

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APPBP1 Antibody (YA2131) Related Classifications

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