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STAT1 Antibody (YA667)

(Synonyms: Signal transducer and activator of transcription 1-alpha/beta, Transcription factor ISGF-3 components p91/p84, STAT1)
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Based on 1 Customer Validation

STAT1 Antibody (YA667) is a Mouse-derived and non-conjugated IgG2b monoclonal antibody, targeting to STAT1.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG

  • Application:

    WB, ICC/IF

  • Reactivity :

    Human, Mouse

  • Formulation:

    Supplied in 1*PBS (pH7.4), 0.2% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Conjugation:
    Non-conjugated
ALL WB ICC
  • Experimental Validation Results for STAT1 Antibody (YA667)
  • Experimental Validation Results for STAT1 Antibody (YA667)
  • Experimental Validation Results for STAT1 Antibody (YA667)
  • STAT1 Antibody (YA667) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Applications

Application
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
Dilution Ratio 1:1000 1:100-1:200

Product Details

Description

STAT1 Antibody (YA667) is a Mouse-derived and non-conjugated IgG2b monoclonal antibody, targeting to STAT1.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human, Mouse
  • Observed Molecular Weight
    Observed band size: 87/83 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 87 kDa
Species Reactivity Database
Immunogen

Synthetic peptide corresponding to Human STAT1.AA range:71-270.

Sensitivity

Endogenous

Purification

Protein A affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3102440

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 1*PBS (pH7.4), 0.2% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for STAT1 Antibody (YA667)
    Western blot analysis of extracts from A549 (lane 2, 20ug), SK-Br-3 (lane 3, 20ug), MCF-7 (lane 4, 20ug) and HT-29 (lane 5, 20ug) using STAT1 Antibody (HY-P80341). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for STAT1 Antibody (YA667)
    Immunocytochemistry analysis of HeLa cells labeling STAT1 with STAT1 Antibody (HY-P80341) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with STAT1 Antibody (HY-P80341) at 1/50 dilution in BSA for Immunol Staining at 4 ℃ overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for STAT1 Antibody (YA667)
    Immunocytochemistry analysis of MCF-7 cells labeling STAT1 with STAT1 Antibody (HY-P80341) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with STAT1 Antibody (HY-P80341) at 1/50 dilution in BSA for Immunol Staining at 4 ℃ overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002,Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

  • Function

    Signal transducer and activator of transcription 1 (STAT1) is a critical transcription factor that mediates cellular responses to interferons and cytokines by undergoing tyrosine phosphorylation, dimerization, and nuclear translocation to regulate target gene expression[1][2]. Mechanistically, STAT1 modulates the JAK/STAT signaling pathway, influencing immune regulation, apoptosis, and inflammation, and it can inhibit the Ras-MAPK pathway and Rho GTPase expression, thereby controlling cell proliferation and adhesion[3]. STAT1 exists as functionally distinct α and β isoforms, which differ in transcriptional activity and protein stability; STAT1β specifically enhances tumor immune surveillance by promoting CD8+ T-cell infiltration and suppressing M2 macrophage polarization in ovarian cancer[4]. In disease models, aberrant STAT1 activation contributes to epithelial ovarian cancer progression, airway inflammation, and spinal cord injury, while targeted inhibition of STAT1 reduces apoptosis and inflammatory responses[5][6][7]. Compared with other STAT family members, STAT1 exhibits unique regulatory interactions, such as selective inhibition by PIAS1 and differential phosphorylation at Y701 and S727, which modulate both canonical and non-canonical pathways[1][3]. Small-molecule inhibitors and agonists, including fostamatinib, MK256, and 15dPGJ2, have been shown to modulate STAT1 activity in vitro and in vivo, offering tools for experimental intervention and potential therapeutic applications[8][9][2]. These findings position STAT1 as a key regulator of immune and stress responses, distinct from related isoforms, and a targetable node for experimental and clinical studies.

  • Subcellular Localization

    Cytoplasm; Nucleus

  • Isoforms & Post-Translational Modification

    STAT1 has 2 isomers: P42224-1: 87335 Da (predicted); P42224-2: 83043 Da (predicted).
    Deubiquitinated by USP13.
    Phosphorylated on tyrosine and serine residues in response to a variety of cytokines/growth hormones including IFN-alpha, IFN-gamma, PDGF and EGF.
    Activated KIT promotes phosphorylation on tyrosine residues and subsequent translocation to the nucleus.
    Upon EGF stimulation, phosphorylation on Tyr-701 (lacking in beta form) by JAK1, JAK2 or TYK2 promotes dimerization and subsequent translocation to the nucleus.
    Growth hormone (GH) activates STAT1 signaling only via JAK2.
    Tyrosine phosphorylated in response to constitutively activated FGFR1, FGFR2, FGFR3 and FGFR4.
    Phosphorylation on Ser-727 by several kinases including MAPK14,
    ERK1/2, CAMK2/CAMKII and CK2 in response to IFN-gamma stimulation, is required for maximal transcriptional activity.
    Phosphorylated on Ser-727 by CAMK2/CAMKII in response to IFN-gamma stimulation and calcium mobilization, promoting activity.
    Phosphorylated by CAMK2/CAMKII in response to IFN-beta stimulation and calcium mobilization in epithelial cells, promoting activity.
    Phosphorylation on Ser-727 promotes sumoylation though increasing interaction with PIAS.
    Phosphorylation on Ser-727 by PRKCD induces apoptosis in response to DNA-damaging agents.
    Phosphorylated on tyrosine residues when PTK2/FAK1 is activated.
    Dephosphorylation on tyrosine residues by PTPN2 negatively regulates interferon-mediated signaling.
    Upon viral infection or IFN induction, phosphorylation on Ser-708 occurs much later than phosphorylation on Tyr-701 and is required for the binding of ISGF3 on the ISREs of a subset of IFN-stimulated genes IKBKE-dependent.
    Phosphorylation at Tyr-701 and Ser-708 are mutually exclusive, phosphorylation at Ser-708 requires previous dephosphorylation of Tyr-701.
    Phosphorylation at Thr-749 by IKBKB/IKKB promotes transcriptional activation of ARID5A and IL12B by STAT1.
    Sumoylated with SUMO1, SUMO2 and SUMO3.
    ISGylated.
    Mono-ADP-ribosylated at Glu-657 and Glu-705 by PARP14.
    Monomethylated at Lys-525 by SETD2; monomethylation is necessary for phosphorylation at Tyr-701, translocation into the nucleus and activation of the antiviral defense.
    'Lys-63'-linked polyubiquitinated at Lys-110 by RNF220; leading to enhanced STAT1 activation and innate immune responses.
    Ubiquitinated by Herpes simplex virus 2 E3 ubiquitin ligase ICP22.

  • Subunit

    Isoform alpha homodimerizes upon IFN-gamma induced phosphorylation. Heterodimer with STAT2 upon IFN-alpha/beta induced phosphorylation.

  • SwissProt ID

    P42224

  • Gene ID
    6772 [NCBI]
  • Synonyms

    Signal transducer and activator of transcription 1-alpha/beta, Transcription factor ISGF-3 components p91/p84, STAT1

  • Research Field

    Signal Transduction

[1]. Chen CW, et al. Inhibition of IFN-gamma-mediated inducible nitric oxide synthase induction by the peroxisome proliferator-activated receptor gamma agonist, 15-deoxy-delta 12,14-prostaglandin J2, involves inhibition of the upstream Janus kinase/STAT1 signaling pathway. J Immunol. 2003 Jul 15;171(2):979-88. [Content Brief]

[2]. Guo R, et al. Fostamatinib, a Spleen Tyrosine Kinase Inhibitor, Exerts Anti-Inflammatory Activity via Inhibiting STAT1/3 Signaling Pathways. J Inflamm Res. 2024 Nov 25;17:9757-9771. [Content Brief]

[3]. Huang XP, et al. miR-135a inhibits airway inflammatory response in asthmatic mice via regulating JAK/STAT signaling pathway. Braz J Med Biol Res. 2021 Jan 15;54(3):e10023. [Content Brief]

[4]. Lan N, et al. STAT1β modulates the tumor immune microenvironment to improve prognosis in ovarian cancer: a comprehensive study of transcriptional and protein expression differences. J Ovarian Res. 2025 Aug 23;18(1):192. [Content Brief]

[5]. Lee JC, et al. MK256 is a novel CDK8 inhibitor with potent antitumor activity in AML through downregulation of the STAT pathway. Oncotarget. 2022 Nov 2;13:1217-1236. [Content Brief]

[6]. Liu B, et al. Inhibition of Stat1-mediated gene activation by PIAS1. Proc Natl Acad Sci U S A. 1998 Sep 1;95(18):10626-31. [Content Brief]

[7]. Tian X, et al. Physical interaction of STAT1 isoforms with TGF-β receptors leads to functional crosstalk between two signaling pathways in epithelial ovarian cancer. J Exp Clin Cancer Res. 2018 May 11;37(1):103. [Content Brief]

[8]. Wang S, et al. Stat1 is an inhibitor of Ras-MAPK signaling and Rho small GTPase expression with implications in the transcriptional signature of Ras transformed cells. Cell Cycle. 2009 Jul 1;8(13):2070-9. [Content Brief]

[9]. Wu YX, et al. STAT1 inhibitor alleviates spinal cord injury by decreasing apoptosis. Genet Mol Res. 2016 Mar 28;15(1). [Content Brief]

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STAT1 Antibody (YA667) Related Classifications

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100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    STAT1 Antibody (YA667) purchased from MedChemExpress. Usage Cited in:

    MedChemExpress Validation
     Western blot analysis of extracts from A549 (lane 2, 20ug), SK-Br-3 (lane 3, 20ug), MCF-7 (lane 4, 20ug) and HT-29 (lane 5, 20ug) using STAT1 Antibody (HY-P80341). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004, 1/10,000) was used for 1 hour at room temperature.

  • 1/16

    STAT1 Antibody (YA667) purchased from MedChemExpress. Usage Cited in:

    MedChemExpress Validation
     Immunocytochemistry analysis of HeLa cells labeling STAT1 with STAT1 Antibody (HY-P80341) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with STAT1 Antibody (HY-P80341) at 1/50 dilution in BSA for Immunol Staining at 4 ℃ overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    STAT1 Antibody (YA667) purchased from MedChemExpress. Usage Cited in:

    MedChemExpress Validation
     Immunocytochemistry analysis of MCF-7 cells labeling STAT1 with STAT1 Antibody (HY-P80341) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with STAT1 Antibody (HY-P80341) at 1/50 dilution in BSA for Immunol Staining at 4 ℃ overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002,Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • STAT1 Antibody (YA667) Product Image
    1/16
  • Experimental Validation Results for STAT1 Antibody (YA667)
  • Experimental Validation Results for STAT1 Antibody (YA667)
  • Experimental Validation Results for STAT1 Antibody (YA667)
  • STAT1 Antibody (YA667) Product Image
  • Experimental Validation Results for STAT1 Antibody (YA667)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from A549 (lane 2, 20ug), SK-Br-3 (lane 3, 20ug), MCF-7 (lane 4, 20ug) and HT-29 (lane 5, 20ug) using STAT1 Antibody (HY-P80341). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for STAT1 Antibody (YA667)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of HeLa cells labeling STAT1 with STAT1 Antibody (HY-P80341) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with STAT1 Antibody (HY-P80341) at 1/50 dilution in BSA for Immunol Staining at 4 ℃ overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for STAT1 Antibody (YA667)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of MCF-7 cells labeling STAT1 with STAT1 Antibody (HY-P80341) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with STAT1 Antibody (HY-P80341) at 1/50 dilution in BSA for Immunol Staining at 4 ℃ overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002,Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for STAT1 Antibody (YA667)
  • Experimental Validation Results for STAT1 Antibody (YA667)
  • Experimental Validation Results for STAT1 Antibody (YA667)
  • STAT1 Antibody (YA667) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from A549 (lane 2, 20ug), SK-Br-3 (lane 3, 20ug), MCF-7 (lane 4, 20ug) and HT-29 (lane 5, 20ug) using STAT1 Antibody (HY-P80341). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004, 1/10,000) was used for 1 hour at room temperature.

  • STAT1 Antibody (YA667) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of HeLa cells labeling STAT1 with STAT1 Antibody (HY-P80341) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with STAT1 Antibody (HY-P80341) at 1/50 dilution in BSA for Immunol Staining at 4 ℃ overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • STAT1 Antibody (YA667) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of MCF-7 cells labeling STAT1 with STAT1 Antibody (HY-P80341) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with STAT1 Antibody (HY-P80341) at 1/50 dilution in BSA for Immunol Staining at 4 ℃ overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002,Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • STAT1 Antibody (YA667) Product Image WB
  • STAT1 Antibody (YA667) Product Image ICC
  • STAT1 Antibody (YA667) Product Image ICC