Phospho-Beclin 1 (Ser30) Antibody (YA9814)
(Synonyms: BECN1)
Based on 1 Customer Validation
Phospho-Beclin 1 (Ser30) Antibody (YA9814) is a Rabbit-derived and non-conjugated IgG Recombinant, Monoclonal antibody, targeting to Phospho-Beclin 1 (Ser30).
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB
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Reactivity :
Human, Mouse, Rat
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Formulation:
Supplied in 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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|---|---|
| Dilution Ratio | 1:10000 |
Product Details
Phospho-Beclin 1 (Ser30) Antibody (YA9814) is a Rabbit-derived and non-conjugated IgG Recombinant, Monoclonal antibody, targeting to Phospho-Beclin 1 (Ser30).
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Host Rabbit
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Species ReactivityHuman, Mouse, Rat
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Observed Molecular WeightObserved band size: 57 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 52 kDa
Synthetic phosphopeptide corresponding to residues surrounding Ser30 of human Beclin 1.
Endogenous
affinity purified
Non-conjugated
Phosphorylated
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.
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Concentration
Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
Beclin 1 plays a central role in autophagy. Acts as a core subunit of the PI3K complex that mediates formation of phosphatidylinositol 3-phosphate; different complex forms are believed to play a role in multiple membrane trafficking pathways: PI3KC3-C1 is involved in initiation of autophagosomes and PI3KC3-C2 in maturation of autophagosomes and endocytosis. Involved in regulation of degradative endocytic trafficking and required for the abscission step in cytokinesis, probably in the context of PI3KC3-C2. Essential for the formation of PI3KC3-C2 but not PI3KC3-C1 PI3K complex forms. Involved in endocytosis. May play a role in antiviral host defense[1][2][3][4][5][6][7][8][9][10][11].
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Subcellular Localization
Cytoplasm; Golgi apparatus, trans-Golgi network membrane; Endosome membrane; Endoplasmic reticulum membrane; Mitochondrion membrane; Endosome; Cytoplasmic vesicle, autophagosome
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Expression
Tissue_Specificity: Ubiquitous. -
Isoforms & Post-Translational Modification
Phospho-Beclin 1 has an amino acid length of 450, molecular weight is 51896 Da.
Phosphorylation at Thr-119 by DAPK1 reduces its interaction with BCL2 and BCL2L1 and promotes induction of autophagy. In response to autophagic stimuli, phosphorylated at serine residues by AMPK in an ATG14-dependent manner, and this phosphorylation is critical for maximally efficient autophagy. -
Subunit
A homodimeric form is proposed to exist; this metastable form readily transits to ATG14- or UVRAG-containing complexes with BECN1:UVRAG being more stable than BECN1:ATG14 (By similarity).
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SwissProt ID
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Synonyms
BECN1
Documentation
References
[1]. Wei Y, et al. JNK1-mediated phosphorylation of Bcl-2 regulates starvation-induced autophagy. Mol Cell. 2008 Jun 20;30(6):678-88. [Content Brief]
[2]. Strappazzon F, et al. Mitochondrial BCL-2 inhibits AMBRA1-induced autophagy. EMBO J. 2011 Apr 6;30(7):1195-208. [Content Brief]
[3]. Margariti A, et al. XBP1 mRNA splicing triggers an autophagic response in endothelial cells through BECLIN-1 transcriptional activation. J Biol Chem. 2013 Jan 11;288(2):859-72. [Content Brief]
[4]. Xia P, et al. WASH inhibits autophagy through suppression of Beclin 1 ubiquitination. EMBO J. 2013 Oct 16;32(20):2685-96. [Content Brief]
[5]. Xu C, et al. Regulation of autophagy by E3 ubiquitin ligase RNF216 through BECN1 ubiquitination. Autophagy. 2014;10(12):2239-50. [Content Brief]
[6]. Ashkenazi A, et al. Polyglutamine tracts regulate beclin 1-dependent autophagy. Nature. 2017 May 4;545(7652):108-111. [Content Brief]
[7]. Wyatt S, et al. Epstein-Barr Virus Encoded BCL2, BHRF1, Downregulates Autophagy by Noncanonical Binding of BECN1. Biochemistry. 2023 Oct 17;62(20):2934-2951. [Content Brief]
[8]. Sagona AP, et al. PtdIns(3)P controls cytokinesis through KIF13A-mediated recruitment of FYVE-CENT to the midbody. Nat Cell Biol. 2010 Apr;12(4):362-71. [Content Brief]
[9]. Thoresen SB, et al. A phosphatidylinositol 3-kinase class III sub-complex containing VPS15, VPS34, Beclin 1, UVRAG and BIF-1 regulates cytokinesis and degradative endocytic traffic. Exp Cell Res. 2010 Dec 10;316(20):3368-78. [Content Brief]
[10]. Liu K, et al. Negative regulation of phosphatidylinositol 3-phosphate levels in early-to-late endosome conversion. J Cell Biol. 2016 Jan 18;212(2):181-98. [Content Brief]
[11]. McKnight NC, et al. Beclin 1 is required for neuron viability and regulates endosome pathways via the UVRAG-VPS34 complex. PLoS Genet. 2014 Oct;10(10):e1004626. [Content Brief]
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