IGFBP1 Antibody
(Synonyms: IBP1, IGFBP1, Insulin-like growth factor-binding protein 1, IBP-1, IGF-binding protein 1, IGFBP-1, Placental protein 12, PP12)IGFBP1 Antibody is a Rabbit-derived and non-conjugated IgG Polyclonal antibody, targeting to IGFBP1.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, IHC-P, ICC/IF
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Reactivity :
Human, Monkey
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Formulation:
Supplied in PBS (pH 7.4), containing 30% glycerol, and 0.01% sodium azide.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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IHC-P
IHC-P: Immunohistochemistry-Paraffin
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ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
|---|---|---|---|
| Dilution Ratio | 1:1000-2000 | 1:100-200 | 1:50-200 |
Product Details
IGFBP1 Antibody is a Rabbit-derived and non-conjugated IgG Polyclonal antibody, targeting to IGFBP1.
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Host Rabbit
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Clonality Polyclonal
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Species ReactivityHuman, Monkey
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Observed Molecular WeightObserved band size: 36 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 27 kDa
Synthetic peptide corresponding to the center region of human IGFBP1.
Endogenous
affinity purified.
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in PBS (pH 7.4), containing 30% glycerol, and 0.01% sodium azide.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
IGFBP1 is a Multifunctional protein that plays a critical role in regulating the availability of IGFs such as IGF1 and IGF2 to their receptors and thereby regulates IGF-mediated cellular processes including cell migration, proliferation, differentiation or apoptosis in a cell-type specific manner. Also plays a positive role in cell migration by interacting with integrin ITGA5:ITGB1 through its RGD motif. Mechanistically, binding to integrins leads to activation of focal adhesion kinase/PTK2 and stimulation of the mitogen-activated protein kinase (MAPK) pathway. Regulates cardiomyocyte apoptosis by suppressing HIF-1alpha/HIF1A ubiquitination and subsequent degradation (By similarity)[1][2][3].
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Subcellular Localization
Secreted
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Isoforms & Post-Translational Modification
IGFBP1 has an amino acid length of 259, molecular weight is 27904 Da.发生磷酸化，可能由酪蛋白激酶 II 介导。磷酸化会改变该蛋白对胰岛素样生长因子(IGFs)的亲和力。在羊水中，未修饰的蛋白是含量最高的形式，而单磷酸化、双磷酸化、三磷酸化和四磷酸化形式的含量依次递减。磷酸化状态可能影响其对蛋白水解的易感性IGFBP1 的氨基酸个数为 259 个，分子量为 27904 Da。Phosphorylated; probably by casein kinase II. Phosphorylation alters the affinity of the protein for IGFs. In amniotic fluid, the unmodified protein is the most abundant form, while mono-, bi-, tri- and tetraphosphorylated forms are present in decreasing amounts. The phosphorylation state may influence the propensity to proteolysis
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Subunit
Binds equally well IGF1 and IGF2.
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SwissProt ID
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Synonyms
IBP1, IGFBP1, Insulin-like growth factor-binding protein 1, IBP-1, IGF-binding protein 1, IGFBP-1, Placental protein 12, PP12
Documentation
[1]. Gleeson LM, et al. Insulin-like growth factor-binding protein 1 stimulates human trophoblast migration by signaling through alpha 5 beta 1 integrin via mitogen-activated protein Kinase pathway. J Clin Endocrinol Metab. 2001 Jun;86(6):2484-93. [Content Brief]
[2]. Sala A, et al. Structure and properties of the C-terminal domain of insulin-like growth factor-binding protein-1 isolated from human amniotic fluid. J Biol Chem. 2005 Aug 19;280(33):29812-9. [Content Brief]
[3]. Jones JI, et al. Insulin-like growth factor binding protein 1 stimulates cell migration and binds to the alpha 5 beta 1 integrin by means of its Arg-Gly-Asp sequence. Proc Natl Acad Sci U S A. 1993 Nov 15;90(22):10553-7. [Content Brief]
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