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TNKS1 Antibody (YA6805)

(Synonyms: ADP-ribosyltransferase diphtheria toxin-like 5 antibody; OTTHUMP00000115550 antibody; OTTHUMP00000224675 antibody; PARP 5a antibody; PARP5A antibody; PARPL antibody; pART5 antibody; Poly [ADP-ribose] polymerase 5A antibody; TANK 1 antibody; TANK1 antibody; ADP-ribosyltransferase diphtheria toxin-like 5 antibody; OTTHUMP00000115550 antibody; OTTHUMP00000224675 antibody; PARP 5a antibody; PARP5A antibody; PARPL antibody; pART5 antibody; Poly [ADP-ribose] polymerase 5A antibody; TANK 1 antibody; TANK1 antibody; Tankyrase 1 antibody; Tankyrase I antibody; Tankyrase TRF1 interacting ankyrin related ADP ribose polymerase antibody; Tankyrase-1 antibody; Tankyrase1 antibody; TankyraseI antibody; TIN 1 antibody; TIN1 antibody; TINF 1 antibody; TINF1 antibody; TNKS 1 antibody; TNKS antibody; TNKS-1 antibody; TNKS1 antibody; TNKS1_HUMAN antibody; TRF1 interacting ankyrin related ADP ribose polymerase antibody; TRF1-interacting ankyrin-related ADP-ribose polymerase antibody; )
Customer Review

Based on 1 Customer Validation

TNKS1 Antibody (YA6805) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to TNKS1.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for TNKS1 Antibody (YA6805)
  • TNKS1 Antibody (YA6805) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
Dilution Ratio 1:1000

Product Details

Description

TNKS1 Antibody (YA6805) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to TNKS1.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 150 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 142 kDa
Immunogen

Recombinant protein within human TNKS1 aa 151-650/1,327.

Purification

affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3719263

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for TNKS1 Antibody (YA6805)
    Western blot analysis of extracts from Hela (lane 2(20μg), jurkat (lane 3(20μg), NIH3T3 (lane 4(20μg), HepG2 (lane 5(20μg) using TNKS1 Antibody（HY-P87112). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001 ,1/10,000) was used for 1 hour at room temperature.

Background

  • Function

    TNKS1 is a Poly-ADP-ribosyltransferase involved in various processes such as Wnt signaling pathway, telomere length and vesicle trafficking. Acts as an activator of the Wnt signaling pathway by mediating poly-ADP-ribosylation (PARsylation) of AXIN1 and AXIN2, 2 key components of the beta-catenin destruction complex: poly-ADP-ribosylated target proteins are recognized by RNF146, which mediates their ubiquitination and subsequent degradation. Also mediates PARsylation of BLZF1 and CASC3, followed by recruitment of RNF146 and subsequent ubiquitination. Mediates PARsylation of TERF1, thereby contributing to the regulation of telomere length. Involved in centrosome maturation during prometaphase by mediating PARsylation of HEPACAM2/MIKI. May also regulate vesicle trafficking and modulate the subcellular distribution of SLC2A4/GLUT4-vesicles. May be involved in spindle pole assembly through PARsylation of NUMA1. Stimulates 26S proteasome activity[1][2][3][4][5][6][7][8][9].

  • Subcellular Localization

    Cytoplasm,Golgi apparatus membrane,Cytoplasm, cytoskeleton, microtubule organizing center, centrosome,Nucleus, nuclear pore complex,Chromosome, telomere,Cytoplasm, cytoskeleton, spindle pole

  • Expression


    Tissue_Specificity: Ubiquitous; highest levels in testis

  • Isoforms & Post-Translational Modification

    O95271 has two isomers: O95271-1: 142039 Da (predicted); O95271-2: 67203 Da (predicted).
    Phosphorylated on serine residues by MAPK kinases upon insulin stimulation丨Ubiquitinated by RNF146 when auto-poly-ADP-ribosylated, leading to its degradation丨ADP-ribosylated (-auto)

  • Subunit

    Oligomerizes and associates with TNKS2

  • SwissProt ID

    O95271

  • Gene ID
    8658 [NCBI]
  • Synonyms

    ADP-ribosyltransferase diphtheria toxin-like 5 antibody; OTTHUMP00000115550 antibody; OTTHUMP00000224675 antibody; PARP 5a antibody; PARP5A antibody; PARPL antibody; pART5 antibody; Poly [ADP-ribose] polymerase 5A antibody; TANK 1 antibody; TANK1 antibody; ADP-ribosyltransferase diphtheria toxin-like 5 antibody; OTTHUMP00000115550 antibody; OTTHUMP00000224675 antibody; PARP 5a antibody; PARP5A antibody; PARPL antibody; pART5 antibody; Poly [ADP-ribose] polymerase 5A antibody; TANK 1 antibody; TANK1 antibody; Tankyrase 1 antibody; Tankyrase I antibody; Tankyrase TRF1 interacting ankyrin related ADP ribose polymerase antibody; Tankyrase-1 antibody; Tankyrase1 antibody; TankyraseI antibody; TIN 1 antibody; TIN1 antibody; TINF 1 antibody; TINF1 antibody; TNKS 1 antibody; TNKS antibody; TNKS-1 antibody; TNKS1 antibody; TNKS1_HUMAN antibody; TRF1 interacting ankyrin related ADP ribose polymerase antibody; TRF1-interacting ankyrin-related ADP-ribose polymerase antibody;

TNKS1 Antibody (YA6805) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from Hela (lane 2(20μg), jurkat (lane 3(20μg), NIH3T3 (lane 4(20μg), HepG2 (lane 5(20μg) using TNKS1 Antibody（HY-P87112). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001 ,1/10,000) was used for 1 hour at room temperature.

  • TNKS1 Antibody (YA6805) Product Image
    1/16
  • Experimental Validation Results for TNKS1 Antibody (YA6805)
  • TNKS1 Antibody (YA6805) Product Image
  • Experimental Validation Results for TNKS1 Antibody (YA6805)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from Hela (lane 2(20μg), jurkat (lane 3(20μg), NIH3T3 (lane 4(20μg), HepG2 (lane 5(20μg) using TNKS1 Antibody（HY-P87112). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001 ,1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for TNKS1 Antibody (YA6805)
  • TNKS1 Antibody (YA6805) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from Hela (lane 2(20μg), jurkat (lane 3(20μg), NIH3T3 (lane 4(20μg), HepG2 (lane 5(20μg) using TNKS1 Antibody（HY-P87112). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001 ,1/10,000) was used for 1 hour at room temperature.

  • TNKS1 Antibody (YA6805) Product Image WB