TNKS1 Antibody (YA6805)

Cat. No.: HY-P87112: Data Sheet COA
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TNKS1 Antibody (YA6805) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to TNKS1.

For research use only. We do not sell to patients.

Size	Stock	Quantity
10 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
250 μL	Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Verification Image
Background
Documentation

Description

TNKS1 Antibody (YA6805) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to TNKS1.

Host

Rabbit

Clonality

Recombinant,Monoclonal

Molecular Weight

Predicted band size: 142 kDa;

Observed band size: 150 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

O95271

Gene ID

8658 [NCBI]

Immunogen

Recombinant protein within human TNKS1 aa 151-650/1,327.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:1000

Purity	affinity purified.	Conjugation	Non-conjugated
Modification	Unmodified	Isotype	IgG

Appearance

Liquid

Formulation

Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Verification Image

Western blot analysis of extracts from Hela (lane 2(20μg), jurkat (lane 3(20μg), NIH3T3 (lane 4(20μg), HepG2 (lane 5(20μg) using TNKS1 Antibody（HY-P87112). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001 ,1/10,000) was used for 1 hour at room temperature.

Background

Function：Poly-ADP-ribosyltransferase involved in various processes such as Wnt signaling pathway, telomere length and vesicle trafficking (PubMed:10988299, PubMed:11739745, PubMed:16076287, PubMed:19759537, PubMed:21478859, PubMed:22864114, PubMed:23622245, PubMed:25043379, PubMed:28619731). Acts as an activator of the Wnt signaling pathway by mediating poly-ADP-ribosylation (PARsylation) of AXIN1 and AXIN2, 2 key components of the beta-catenin destruction complex: poly-ADP-ribosylated target proteins are recognized by RNF146, which mediates their ubiquitination and subsequent degradation (PubMed:19759537, PubMed:21478859). Also mediates PARsylation of BLZF1 and CASC3, followed by recruitment of RNF146 and subsequent ubiquitination (PubMed:21478859). Mediates PARsylation of TERF1, thereby contributing to the regulation of telomere length (PubMed:11739745). Involved in centrosome maturation during prometaphase by mediating PARsylation of HEPACAM2/MIKI (PubMed:22864114). May also regulate vesicle trafficking and modulate the subcellular distribution of SLC2A4/GLUT4-vesicles (PubMed:10988299). May be involved in spindle pole assembly through PARsylation of NUMA1 (PubMed:16076287). Stimulates 26S proteasome activity (PubMed:23622245)

Subcellular Localization：Cytoplasm,Golgi apparatus membrane,Cytoplasm, cytoskeleton, microtubule organizing center, centrosome,Nucleus, nuclear pore complex,Chromosome, telomere,Cytoplasm, cytoskeleton, spindle pole

Expression：
Tissue_Specificity: Ubiquitous; highest levels in testis

Isoforms & Post-Translational Modification：O95271 has two isomers: O95271-1: 142039 Da (predicted); O95271-2: 67203 Da (predicted).
Phosphorylated on serine residues by MAPK kinases upon insulin stimulation丨Ubiquitinated by RNF146 when auto-poly-ADP-ribosylated, leading to its degradation丨ADP-ribosylated (-auto)

Subunit：Oligomerizes and associates with TNKS2

RRID

AB_3719263

Synonyms

ADP-ribosyltransferase diphtheria toxin-like 5 antibody; OTTHUMP00000115550 antibody; OTTHUMP00000224675 antibody; PARP 5a antibody; PARP5A antibody; PARPL antibody; pART5 antibody; Poly [ADP-ribose] polymerase 5A antibody; TANK 1 antibody; TANK1 antibody; ADP-ribosyltransferase diphtheria toxin-like 5 antibody; OTTHUMP00000115550 antibody; OTTHUMP00000224675 antibody; PARP 5a antibody; PARP5A antibody; PARPL antibody; pART5 antibody; Poly [ADP-ribose] polymerase 5A antibody; TANK 1 antibody; TANK1 antibody; Tankyrase 1 antibody; Tankyrase I antibody; Tankyrase TRF1 interacting ankyrin related ADP ribose polymerase antibody; Tankyrase-1 antibody; Tankyrase1 antibody; TankyraseI antibody; TIN 1 antibody; TIN1 antibody; TINF 1 antibody; TINF1 antibody; TNKS 1 antibody; TNKS antibody; TNKS-1 antibody; TNKS1 antibody; TNKS1_HUMAN antibody; TRF1 interacting ankyrin related ADP ribose polymerase antibody; TRF1-interacting ankyrin-related ADP-ribose polymerase antibody;

Documentation

Select Batch:

Data Sheet (231 KB) SDS (254 KB)

COA (206 KB)

User Guide for Antibodies (1077 KB)