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Laminin alpha 3 Antibody (YA1548)

(Synonyms: LAMA3; Laminin 5 alpha3; E170; LAMNA; LOCS; laminin alpha 3; Laminin A3)
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Based on 1 Customer Validation

Laminin alpha 3 Antibody (YA1548) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Laminin alpha 3.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, ICC/IF

  • Reactivity :

    Human

  • Formulation:

    Supplied in Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for Laminin alpha 3 Antibody (YA1548)
  • Experimental Validation Results for Laminin alpha 3 Antibody (YA1548)
  • Laminin alpha 3 Antibody (YA1548) Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
Dilution Ratio 1:500-1:1000 1:50-1:200

Product Details

Description

Laminin alpha 3 Antibody (YA1548) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Laminin alpha 3.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 367 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 367 kDa
Species Reactivity Database

Entrez Gene: 3909 Human

SwissProt: Q16787 Human

Immunogen

A synthesized peptide derived from human LAMA3 aa92-143.

Sensitivity

Endogenous

Purification

Affinity Chromatography

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3103904

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Laminin alpha 3 Antibody (YA1548)
    Immunocytochemistry analysis of HepG2 cells labeling Laminin alpha 3 with Laminin alpha 3 Antibody (HY-P81803) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with Laminin alpha 3 Antibody (HY-P81803) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Laminin alpha 3 Antibody (YA1548)
    Immunocytochemistry analysis of HepG2 cells labeling Laminin alpha 3 with Laminin alpha 3 Antibody (HY-P81803) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with Laminin alpha 3 Antibody (HY-P81803) at 1/200 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

  • Function

    Laminin alpha 3 is a Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components; Laminin-5 is thought to be involved in (1) cell adhesion via integrin alpha-3/beta-1 in focal adhesion and integrin alpha-6/beta-4 in hemidesmosomes, (2) signal transduction via tyrosine phosphorylation of pp125-FAK and p80, (3) differentiation of keratinocytes

  • Subcellular Localization

    Secreted, extracellular space, extracellular matrix, basement membrane

  • Expression


    Tissue_specificity:Skin; respiratory, urinary, and digestive epithelia and in other specialized tissues with prominent secretory or protective functions. Epithelial basement membrane, and epithelial cell tongue that migrates into a wound bed. A differential and focal expression of the subunit alpha-3 is observed in the CNS

    Induction:Laminin-5 is up-regulated in wound sites of human skin

  • Isoforms & Post-Translational Modification

    Q16787 has 4 isomers: Q16787-2: 366619 Da (predicted); Q16787-1: 190461 Da (predicted); Q16787-3: 360182 Da (predicted); Q16787-4: 184024 Da (predicted).

  • Subunit

    Laminin is a complex glycoprotein, consisting of three different polypeptide chains (alpha, beta, gamma), which are bound to each other by disulfide bonds into a cross-shaped molecule comprising one long and three short arms with globules at each end.

  • SwissProt ID

    Q16787

  • Gene ID
    3909 [NCBI]
  • Synonyms

    LAMA3; Laminin 5 alpha3; E170; LAMNA; LOCS; laminin alpha 3; Laminin A3

  • Research Field

    Cardiovascular

Laminin alpha 3 Antibody (YA1548) Related Classifications

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100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of HepG2 cells labeling Laminin alpha 3 with Laminin alpha 3 Antibody (HY-P81803) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with Laminin alpha 3 Antibody (HY-P81803) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of HepG2 cells labeling Laminin alpha 3 with Laminin alpha 3 Antibody (HY-P81803) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with Laminin alpha 3 Antibody (HY-P81803) at 1/200 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Laminin alpha 3 Antibody (YA1548) Product Image
    1/16
  • Experimental Validation Results for Laminin alpha 3 Antibody (YA1548)
  • Experimental Validation Results for Laminin alpha 3 Antibody (YA1548)
  • Laminin alpha 3 Antibody (YA1548) Product Image
  • Experimental Validation Results for Laminin alpha 3 Antibody (YA1548)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of HepG2 cells labeling Laminin alpha 3 with Laminin alpha 3 Antibody (HY-P81803) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with Laminin alpha 3 Antibody (HY-P81803) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Laminin alpha 3 Antibody (YA1548)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of HepG2 cells labeling Laminin alpha 3 with Laminin alpha 3 Antibody (HY-P81803) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with Laminin alpha 3 Antibody (HY-P81803) at 1/200 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Laminin alpha 3 Antibody (YA1548)
  • Experimental Validation Results for Laminin alpha 3 Antibody (YA1548)
  • Laminin alpha 3 Antibody (YA1548) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of HepG2 cells labeling Laminin alpha 3 with Laminin alpha 3 Antibody (HY-P81803) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with Laminin alpha 3 Antibody (HY-P81803) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Laminin alpha 3 Antibody (YA1548) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of HepG2 cells labeling Laminin alpha 3 with Laminin alpha 3 Antibody (HY-P81803) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with Laminin alpha 3 Antibody (HY-P81803) at 1/200 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Laminin alpha 3 Antibody (YA1548) Product Image ICC
  • Laminin alpha 3 Antibody (YA1548) Product Image ICC