R-Spondin Antibody (YA5049)(PBS only)
(Synonyms: RSPO1; R-spondin-1; Roof plate-specific spondin-1; hRspo1)R-Spondin Antibody (YA5049) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to R-Spondin.
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Host:
Mouse
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Isotype:
IgG1
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Application:
WB, ELISA
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Reactivity :
Human
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Formulation:
Supplied in PBS, pH 7.4.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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ELISA
ELISA: Enzyme Linked Immunosorbent Assay
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|---|---|---|
| Dilution Ratio | 1:500-2000 | 1:10000 |
Product Details
R-Spondin Antibody (YA5049) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to R-Spondin.
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Host Mouse
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Species ReactivityHuman
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Calculated Molecular Weight Predicted band size: 28.9kDa;
Purified recombinant fragment of R-spondin1 expressed in E. Coli. The exact sequence is proprietary to MCE.
affinity purified
Non-conjugated
IgG1
Product Properties
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Appearance
Liquid
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Formulation
Supplied in PBS, pH 7.4.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
R-Spondin is an Activator of the canonical Wnt signaling pathway by acting as a ligand for LGR4-6 receptors. Upon binding to LGR4-6 (LGR4, LGR5 or LGR6), LGR4-6 associate with phosphorylated LRP6 and frizzled receptors that are activated by extracellular Wnt receptors, triggering the canonical Wnt signaling pathway to increase expression of target genes. Also regulates the canonical Wnt/beta-catenin-dependent pathway and non-canonical Wnt signaling by acting as an inhibitor of ZNRF3, an important regulator of the Wnt signaling pathway. Acts as a ligand for frizzled FZD8 and LRP6. May negatively regulate the TGF-beta pathway. Has a essential roles in ovary determination. Regulates Wnt signaling by antagonizing DKK1/KREM1-mediated internalization of LRP6 through an interaction with KREM1[1][2].
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Subcellular Localization
Secreted; Nucleus
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Expression
Tissue_specificity:Abundantly expressed in adrenal glands, ovary, testis, thyroid and trachea but not in bone marrow, spinal cord, stomach, leukocytes colon, small intestine, prostate, thymus and spleen -
Isoforms & Post-Translational Modification
Q2MKA7 has 3 isomers: Q2MKA7-1: 28959 Da (predicted); Q2MKA7-2: 25975 Da (predicted); Q2MKA7-3: 21775 Da (predicted).
C-, and N-glycosylated. N-glycosylation at Asn-137, negatively influences its secretion and enhancing effect on Wnt/beta-catenin signaling (PubMed:27123103). C-mannosylation at Trp-156 by DPY19L3 is required for its secretion and regulates the enhancing activity of Wnt signaling (PubMed:26764097) -
Subunit
Interacts with the extracellular domain of FZD8 and LRP6. It however does not form a ternary complex with FZD8 and LRP6. Interacts with WNT1. Binds heparin (By similarity). Interacts with ZNRF3; promoting indirect interaction between ZNRF3 and LGR4 and membrane clearance of ZNRF3. Interacts with LGR4, LGR5 and LGR6. Identified in a complex composed of RNF43, LGR5 and RSPO1. Interacts (via FU repeats) with KREM1 (PubMed:17804805)
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SwissProt ID
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Synonyms
RSPO1; R-spondin-1; Roof plate-specific spondin-1; hRspo1
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