GDNF Antibody (YA2491)

Cat. No.: HY-P82746: Data Sheet COA
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User Guide for Antibodies
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GDNF Antibody (YA2491) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to GDNF.

For research use only. We do not sell to patients.

Size	Stock	Quantity
Free Sample	Apply now
10 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
250 μL	Get quote

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GDNF Antibody (YA2491) Featured Recommendations:

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
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Background
Documentation
References

Description

GDNF Antibody (YA2491) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to GDNF.

Host

Rabbit

Clonality

Recombinant,Monoclonal

Molecular Weight

Predicted band size: 24 kDa;

Observed band size: 24 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

P39905

Gene ID

2668 [NCBI]

Immunogen

A synthesized peptide derived from human GDNF aa50-100.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000
FC FC: Flow Cytometry	1:50-1:100

Sensitivity	Endogenous	Purity	Affinity Chromatography
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in rabbit IgG in 10mM PBS, pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Verification Image

ALL WB FC

Western blot analysis of extracts from HepG2(lane 2(40μg),SH-SY5Y(lane 3(40μg), Neuro-2a(lane 4(40μg),C6(lane 5(40μg)using GDNF Antibody (YA2491). Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (1/1000)and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.
Flow cytometric analysis of 1X10⁶ SH-SY5Y cells labeling GDNF Antibody(HY-P82746, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/50 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

Background

Function：Neurotrophic factor that enhances survival and morphological differentiation of dopaminergic neurons and increases their high-affinity dopamine uptake (PubMed:8493557). Acts by binding to its coreceptor, GFRA1, leading to autophosphorylation and activation of the RET receptor (PubMed:10829012, PubMed:25242331, PubMed:31535977). Involved in the development of the neural crest (PubMed:15242795)

Subcellular Localization：Secreted

Expression：
Tissue_specificity:In the brain, predominantly expressed in the striatum with highest levels in the caudate and lowest in the putamen. Isoform 2 is absent from most tissues except for low levels in intestine and kidney. Highest expression of isoform 3 is found in pancreatic islets. Isoform 5 is expressed at very low levels in putamen, nucleus accumbens, prefrontal cortex, amygdala, hypothalamus and intestine. Isoform 3 is up-regulated in the middle temporal gyrus of Alzheimer disease while isoform 2 shows no change

Induction:By cAMP, 12-O-tetradecanoylphorbol-13-acetate (TPA) and FGF2

Isoforms & Post-Translational Modification：P39905 has 5 isomers: P39905-1: 23720 Da (predicted); P39905-2: 20885 Da (predicted); P39905-3: 25466 Da (predicted); P39905-4: 22631 Da (predicted); P39905-5: 18123 Da (predicted).

Subunit：Homodimer; disulfide-linked (PubMed:19478429, PubMed:25242331, PubMed:31535977, PubMed:8493557, PubMed:8988018). Interacts with GFRA1 coreceptor and RET: forms a 2:2:2 ternary complex composed of GDNF ligand, GFRA1 and RET receptor (PubMed:21994944, PubMed:25242331, PubMed:31535977). Interacts (via propeptide) with SORL1 (via N-terminal ectodomain); this interaction affects GDNF-regulated, but not constitutive secretion (PubMed:15364913, PubMed:21994944, PubMed:23333276). Also interacts with SORL1 in complex with GFRA1; this interaction leads to GDNF endocytosis and lysosomal degradation (PubMed:23333276)

Database

Entrez Gene: 2668 Human ; 14573 Mouse ; 25453 Rat

SwissProt: P39905 Human ; P48540 Mouse ; Q07731 Rat

OMIM: 613711 Human

Research Field

Neuroscience

Synonyms

ATF1; ATF2; gdnf; HFB1 GDNF; hGDNF; HSCR3

Documentation

Select Batch:

Data Sheet (232 KB) SDS (251 KB)

COA (205 KB)

User Guide for Antibodies (1077 KB)

References

[1]. /biology-dictionary/huh-7-cell.html [Content Brief]

GDNF Antibody (YA2491) Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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GDNF Antibody (YA2491)

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Background

Documentation

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GDNF Antibody (YA2491) Related Classifications

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