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GDNF Antibody (YA2491)

(Synonyms: ATF1; ATF2; gdnf; HFB1 GDNF; hGDNF; HSCR3)
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Based on 1 Customer Validation

GDNF Antibody (YA2491) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to GDNF.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, FC

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in rabbit IgG in 10mM PBS, pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for GDNF Antibody (YA2491)
  • Experimental Validation Results for GDNF Antibody (YA2491)
  • GDNF Antibody (YA2491) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Data Download
Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
FC Info
FC: Flow Cytometry
Dilution Ratio 1:500-1:1000 1:50-1:100

Product Details

Description

GDNF Antibody (YA2491) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to GDNF.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 24 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 24 kDa
Species Reactivity Database
Immunogen

A synthesized peptide derived from human GDNF aa50-100.

Sensitivity

Endogenous

Purification

Affinity Chromatography

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in rabbit IgG in 10mM PBS, pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for GDNF Antibody (YA2491)
    Western blot analysis of extracts from HepG2(lane 2(40μg),SH-SY5Y(lane 3(40μg), Neuro-2a(lane 4(40μg),C6(lane 5(40μg)using GDNF Antibody (YA2491). Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (1/1000)and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for GDNF Antibody (YA2491)
    Flow cytometric analysis of 1X106 SH-SY5Y cells labeling GDNF Antibody(HY-P82746, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/50 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

Background

  • Function

    GDNF is a Neurotrophic factor that enhances survival and morphological differentiation of dopaminergic neurons and increases their high-affinity dopamine uptake. Acts by binding to its coreceptor, GFRA1, leading to autophosphorylation and activation of the RET receptor. Involved in the development of the neural crest[1][2][3][4][5].

  • Subcellular Localization

    Secreted

  • Expression


    Tissue_specificity:In the brain, predominantly expressed in the striatum with highest levels in the caudate and lowest in the putamen. Isoform 2 is absent from most tissues except for low levels in intestine and kidney. Highest expression of isoform 3 is found in pancreatic islets. Isoform 5 is expressed at very low levels in putamen, nucleus accumbens, prefrontal cortex, amygdala, hypothalamus and intestine. Isoform 3 is up-regulated in the middle temporal gyrus of Alzheimer disease while isoform 2 shows no change

    Induction:By cAMP, 12-O-tetradecanoylphorbol-13-acetate (TPA) and FGF2

  • Isoforms & Post-Translational Modification

    P39905 has 5 isomers: P39905-1: 23720 Da (predicted); P39905-2: 20885 Da (predicted); P39905-3: 25466 Da (predicted); P39905-4: 22631 Da (predicted); P39905-5: 18123 Da (predicted).

  • Subunit

    Homodimer; disulfide-linked (PubMed:19478429, PubMed:25242331, PubMed:31535977, PubMed:8493557, PubMed:8988018). Interacts with GFRA1 coreceptor and RET: forms a 2:2:2 ternary complex composed of GDNF ligand, GFRA1 and RET receptor (PubMed:21994944, PubMed:25242331, PubMed:31535977). Interacts (via propeptide) with SORL1 (via N-terminal ectodomain); this interaction affects GDNF-regulated, but not constitutive secretion (PubMed:15364913, PubMed:21994944, PubMed:23333276). Also interacts with SORL1 in complex with GFRA1; this interaction leads to GDNF endocytosis and lysosomal degradation (PubMed:23333276)

  • SwissProt ID

    P39905

  • Gene ID
    2668 [NCBI]
  • Synonyms

    ATF1; ATF2; gdnf; HFB1 GDNF; hGDNF; HSCR3

  • Research Field

    Neuroscience

GDNF Antibody (YA2491) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from HepG2(lane 2(40μg),SH-SY5Y(lane 3(40μg), Neuro-2a(lane 4(40μg),C6(lane 5(40μg)using GDNF Antibody (YA2491). Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (1/1000)and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • 1/16

    MedChemExpress Validation
     Flow cytometric analysis of 1X106 SH-SY5Y cells labeling GDNF Antibody(HY-P82746, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/50 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

  • GDNF Antibody (YA2491) Product Image
    1/16
  • Experimental Validation Results for GDNF Antibody (YA2491)
  • Experimental Validation Results for GDNF Antibody (YA2491)
  • GDNF Antibody (YA2491) Product Image
  • Experimental Validation Results for GDNF Antibody (YA2491)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from HepG2(lane 2(40μg),SH-SY5Y(lane 3(40μg), Neuro-2a(lane 4(40μg),C6(lane 5(40μg)using GDNF Antibody (YA2491). Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (1/1000)and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for GDNF Antibody (YA2491)
    1/1
    MedChemExpress Validation
     Flow cytometric analysis of 1X106 SH-SY5Y cells labeling GDNF Antibody(HY-P82746, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/50 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).
  • Experimental Validation Results for GDNF Antibody (YA2491)
  • Experimental Validation Results for GDNF Antibody (YA2491)
  • GDNF Antibody (YA2491) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from HepG2(lane 2(40μg),SH-SY5Y(lane 3(40μg), Neuro-2a(lane 4(40μg),C6(lane 5(40μg)using GDNF Antibody (YA2491). Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (1/1000)and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • GDNF Antibody (YA2491) Product Image
    MedChemExpress Validation

    Flow cytometric analysis of 1X106 SH-SY5Y cells labeling GDNF Antibody(HY-P82746, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/50 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

  • GDNF Antibody (YA2491) Product Image WB
  • GDNF Antibody (YA2491) Product Image FC