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Collagen III Antibody (YA6319)

(Synonyms: Collagen alpha-1(III) chain, COL3A1)
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Based on 1 Customer Validation

Collagen III Antibody (YA6319) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Collagen III.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, ICC/IF, IP, ELISA

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for Collagen III Antibody (YA6319)
  • Collagen III Antibody (YA6319) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:20-100 1:1000-5000 1:200-1000 1:5000-20000 1:50-200

Product Details

Description

Collagen III Antibody (YA6319) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Collagen III.

  • Host Rabbit
  • Clonality Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 150 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 138 kDa
Purification

Protein A

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Collagen III Antibody (YA6319)
    Western blot analysis was performed on protein extracts (25 μg) from HepG2 (lane 2), SH-SY5Y (lane 3), HeLa (lane 4), HT-1080 (lane 5), MCF-7 (lane 6), and A549 (lane 7) using Collagen III antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. Thhe primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:2500) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit IgG (H&L) secondary antibody (HY-P8001) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

Background

  • Function

    Collagen III (COL3A1) is a fibrillar extracellular matrix collagen synthesized as pre-procollagen and enriched in extensible tissues, including skin, blood vessels, intestine, uterus, and lung[1]. Mechanistically, collagen III regulates collagen I fibrillogenesis, fibril diameter, extracellular matrix architecture, and tissue mechanical integrity[2][3]. In wound models, collagen III supports re-epithelialization and limits scar-associated collagen fiber alignment, while reduced collagen III increases myofibroblast differentiation and scar deposition[4][5]. In disease models, COL3A1 mutations or deficiency produce vascular and dermal fragility resembling vascular Ehlers-Danlos syndrome[6][7]. Compared with collagen I, collagen III functions less as a dominant tensile scaffold and more as a fibril-network modifier that controls matrix organization, mechanosensing, and repair quality[2][3][4]. For experimental applications, the collagen III N-propeptide cysteine-rich domain attenuates TGFβ signaling and suppresses fibroblast activation, supporting its use as a mechanistic inhibitor tool in fibrosis and scarring studies[8].

  • Subcellular Localization

    Secreted, extracellular space, extracellular matrix

  • Isoforms & Post-Translational Modification

    P02461 has 2 isomers: P02461-1: 138564 Da (predicted); P02461-2: 111907 Da (predicted).
    Proline residues at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains;O-linked glycan consists of a Glc-Gal disaccharide bound to the oxygen atom of a post-translationally added hydroxyl group

  • Subunit

    Trimers of identical alpha 1(III) chains. The chains are linked to each other by interchain disulfide bonds. Trimers are also cross-linked via hydroxylysines. Interacts with ADGRG1 (PubMed:28258187)

  • SwissProt ID

    P02461

  • Gene ID
    1281 [NCBI]
  • Synonyms

    Collagen alpha-1(III) chain, COL3A1

References

[1]. Kuivaniemi H, et al. Type III collagen (COL3A1): Gene and protein structure, tissue distribution, and associated diseases. Gene. 2019 Jul 30;707:151-171. [Content Brief]

[2]. Liu X, et al. Type III collagen is crucial for collagen I fibrillogenesis and for normal cardiovascular development. Proc Natl Acad Sci U S A. 1997 Mar 4;94(5):1852-6. [Content Brief]

[3]. Wang C, et al. Type III collagen is a key regulator of the collagen fibrillar structure and biomechanics of articular cartilage and meniscus. Matrix Biol. 2020 Jan;85-86:47-67. [Content Brief]

[4]. Stewart DC, et al. Type III Collagen Regulates Matrix Architecture and Mechanosensing during Wound Healing. J Invest Dermatol. 2025 Apr;145(4):919-938.e14. [Content Brief]

[5]. Volk SW, et al. Diminished type III collagen promotes myofibroblast differentiation and increases scar deposition in cutaneous wound healing. Cells Tissues Organs. 2011;194(1):25-37. [Content Brief]

[6]. D'hondt S, et al. Type III collagen affects dermal and vascular collagen fibrillogenesis and tissue integrity in a mutant Col3a1 transgenic mouse model. Matrix Biol. 2018 Sep;70:72-83. [Content Brief]

[7]. Schwarze U, et al. Haploinsufficiency for one COL3A1 allele of type III procollagen results in a phenotype similar to the vascular form of Ehlers-Danlos syndrome, Ehlers-Danlos syndrome type IV. Am J Hum Genet. 2001 Nov;69(5):989-1001. [Content Brief]

[8]. Brisson BK, et al. Cysteine-rich domain of type III collagen N-propeptide inhibits fibroblast activation by attenuating TGFβ signaling. Matrix Biol. 2022 May;109:19-33. [Content Brief]

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Collagen III Antibody (YA6319) Related Classifications

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100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis was performed on protein extracts (25 μg) from HepG2 (lane 2), SH-SY5Y (lane 3), HeLa (lane 4), HT-1080 (lane 5), MCF-7 (lane 6), and A549 (lane 7) using Collagen III antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. Thhe primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:2500) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit IgG (H&L) secondary antibody (HY-P8001) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • Collagen III Antibody (YA6319) Product Image
    1/16
  • Experimental Validation Results for Collagen III Antibody (YA6319)
  • Collagen III Antibody (YA6319) Product Image
  • Experimental Validation Results for Collagen III Antibody (YA6319)
    1/1
    MedChemExpress Validation
     Western blot analysis was performed on protein extracts (25 μg) from HepG2 (lane 2), SH-SY5Y (lane 3), HeLa (lane 4), HT-1080 (lane 5), MCF-7 (lane 6), and A549 (lane 7) using Collagen III antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. Thhe primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:2500) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit IgG (H&L) secondary antibody (HY-P8001) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).
  • Experimental Validation Results for Collagen III Antibody (YA6319)
  • Collagen III Antibody (YA6319) Product Image
    MedChemExpress Validation

    Western blot analysis was performed on protein extracts (25 μg) from HepG2 (lane 2), SH-SY5Y (lane 3), HeLa (lane 4), HT-1080 (lane 5), MCF-7 (lane 6), and A549 (lane 7) using Collagen III antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. Thhe primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:2500) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit IgG (H&L) secondary antibody (HY-P8001) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • Collagen III Antibody (YA6319) Product Image WB