Eg5 Antibody (YA776)(PBS only)

Cat. No.: HY-P80647A: Data Sheet User Guide for Antibodies
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Eg5 Antibody (YA776) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to Eg5.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

Eg5 Antibody (YA776) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to Eg5.

Host

Mouse

Molecular Weight

Predicted band size: 119 kDa; Observed band size: 130 kDa；

Species Reactivity

Human

SwissProt ID

P52732

Gene ID

3832 [NCBI]

Immunogen

Synthetic peptide corresponding to Human Eg5.The exact sequence is proprietary to MCE.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:50-1:200
IP IP: Immunoprecipitation	1:20

Purity	affinity purified	Conjugation	Non-conjugated
Isotype	IgG1

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Motor protein required for establishing a bipolar spindle and thus contributing to chromosome congression during mitosis (PubMed:19001501, PubMed:37728657). Required in non-mitotic cells for transport of secretory proteins from the Golgi complex to the cell surface (PubMed:23857769)

Subcellular Localization：Cytoplasm; Cytoplasm, cytoskeleton, spindle pole

Subunit：Interacts with the thyroid hormone receptor in the presence of thyroid hormone. Component of a large chromatin remodeling complex, at least composed of MYSM1, PCAF, RBM10 and KIF11/TRIP5. Interacts (via C-terminus) with the kinase NEK6 in both interphase and mitosis. Interacts with RARRES1 and AGBL2 (PubMed:21303978). Interacts with TPX2 (PubMed:37728657)

Research Field

Signal Transduction

Synonyms

KIF11; EG5; KNSL1; TRIP5; Kinesin-like protein KIF11; Kinesin-like protein 1; Kinesin-like spindle protein HKSP; Kinesin-related motor protein Eg5; Thyroid receptor-interacting protein 5; TR-interacting protein 5; TRIP-5

Documentation

User Guide for Antibodies (1077 KB)