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  5. NDRG2 Antibody (YA3295)

NDRG2 Antibody (YA3295) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to NDRG2.

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Top Publications Citing Use of Products
  • WB: Western Blot;
  • IHC-P: Immunohistochemistry-Paraffin;
  • IHC-F: Immunohistochemistry-Frozen;
  • ICC/IF: Immunocytochemistry/Immunofluorescence;
  • IF-Tissue: Immunofluorescence-Tissue;
  • mIHC: Multiplex Immunohistochemical;
  • IP: Immunoprecipitation;
  • ChIP: Chromatin Immunoprecipitation;
  • FC: Flow Cytometry;
  • ELISA: Enzyme Linked Immunosorbent Assay

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제품 설명

NDRG2 Antibody (YA3295) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to NDRG2.
Host

Rabbit
Clonality

Recombinant, Monoclonal
분자량
Predicted band size: 41 kDa;
Observed band size: 41 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
Species Reactivity
Mouse, Rat
SwissProt ID
Gene ID
Immunogen

A synthetic peptide of human NDRG2
Application &
Dilution Ratio
Application Dilution Ratio
WB
WB: Western Blot
1:500-1:1000
IHC-P
IHC-P: Immunohistochemistry-Paraffin
1:50-1:100
IHC-F
IHC-F: Immunohistochemistry-Frozen
1:50-1:100
ICC/IF
ICC/IF: Immunocytochemistry/Immunofluorescence
1:50-1:200
IP
IP: Immunoprecipitation
1:20
Sensitivity Endogenous Purity Affinity Purified
Conjugation Non-conjugated Modification Unmodified
Isotype IgG  
Appearance

Liquid
Formulation

Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
선적

Shipping with blue ice.
Verification Image
IHC-P

  • Immunohistochemical analysis of paraffin-embedded rat brain tissue using NDRG2 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat brain tissue using NDRG2 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Background
Function：Contributes to the regulation of the Wnt signaling pathway. Down-regulates CTNNB1-mediated transcriptional activation of target genes, such as CCND1, and may thereby act as tumor suppressor. May be involved in dendritic cell and neuron differentiation
Subcellular Localization：Cytoplasm; Cytoplasm, perinuclear region; Cell projection, growth cone
Expression：
Tissue_specificity:Highly expressed in brain, heart, skeletal muscle and salivary gland, and moderately in kidney and liver. Expressed in dendritic cells, but not in other blood cells. Expression levels are low in pancreatic and liver cancer tissues; absent in meningioma. Expressed in low-grade gliomas but present at low levels in glioblastoma. Isoform 1 and isoform 2 are present in brain neurons and up-regulated in Alzheimer disease (at protein level)
Isoforms & Post-Translational Modification：Q9UN36 has 6 isomers: Q9UN36-1: 40798 Da (predicted); Q9UN36-2: 39289 Da (predicted); Q9UN36-3: 39679 Da (predicted); Q9UN36-4: 36052 Da (predicted); Q9UN36-5: 37497 Da (predicted); Q9UN36-6: 40297 Da (predicted).
Subunit：Interacts with CTNNB1
RRID
Database

Entrez Gene: 57447 Human ; 29811 Mouse ; 171114 Rat

SwissProt: Q9UN36 Human ; Q9QYG0 Mouse ; Q8VBU2 Rat

OMIM: 605272 Human

Research Field

Neuroscience
Synonyms
SYLD
각종 서류
SDS (251 KB)

COA (206 KB)

User Guide for Antibodies (1077 KB)

NDRG2 Antibody (YA3295) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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상품명:
NDRG2 Antibody (YA3295)
Cat. No.:
HY-P83550
수량:
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SAFETY DATA SHEET (SDS)

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