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Tyrosinase Antibody (YA2522)

(Synonyms: ATN; CMM8; LB24 AB; SHEP3; SK29 AB; Tumor rejection antigen AB; TYR)
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Based on 1 Customer Validation

Tyrosinase Antibody (YA2522) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Tyrosinase.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, ICC/IF

  • Reactivity :

    Human

  • Formulation:

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for Tyrosinase Antibody (YA2522)
  • Experimental Validation Results for Tyrosinase Antibody (YA2522)
  • Tyrosinase Antibody (YA2522) Product Image
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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
Dilution Ratio 1:500-1:1000 1:50-1:100 1:50-1:200

Product Details

Description

Tyrosinase Antibody (YA2522) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Tyrosinase.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 70-80 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 60 kDa
Species Reactivity Database

Entrez Gene: 7299 Human

SwissProt: P14679 Human

Immunogen

A synthesized peptide derived from human Tyrosinase aa1-300/529.

Sensitivity

Endogenous

Purification

Affinity Chromatography

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3104873

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Tyrosinase Antibody (YA2522)
    Immunocytochemistry analysis of Hela cells labeling Tyrosinase Antibody (HY-P82777) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with Tyrosinase Antibody (HY-P82777) at 1/50 dilution in BSA for Immunol Staining at 4 ℃ Overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Tyrosinase Antibody (YA2522)
    Immunocytochemistry analysis of Hela cells labeling Tyrosinase Antibody (HY-P82777) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with Tyrosinase Antibody (HY-P82777) at 1/200dilution in BSA for Immunol Staining at 4 ℃ Overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

  • Function

    Tyrosinase is a copper-containing metalloprotein belonging to the type-3 copper protein family, together with haemocyanins and catechol oxidases. Tyrosinases are the catalysts in mammals responsible for the formation of melanin in skin and hair color, as well as browning in fruit and vegetables following cell damage.

    Tyrosinases are found in various prokaryotes as well as in plants, fungi, arthropods, and mammals and are responsible for pigmentation, wound healing, radiation protection, and primary immune response. Tyrosinases perform two sequential enzymatic reactions: hydroxylation of monophenols and oxidation of diphenols to form quinones which polymerize spontaneously to melanin. In plants, sponges, and many invertebrates, tyrosinases are important for wound healing and primary immune responses; in arthropods, they play a role in sclerotization, and in bacteria, tyrosinases protect DNA from UV damage.

  • Subcellular Localization

    Melanosome membrane; Single-pass type I membrane protein; Melanosome

  • Expression


    Induction:Increased expression after UVB irradiation

  • Isoforms & Post-Translational Modification

    P14679 has 2 isomers: P14679-1: 60393 Da (predicted); P14679-2: 42914 Da (predicted).
    Glycosylated

  • Subunit

    Forms an OPN3-dependent complex with DCT in response to blue light in melanocytes

  • SwissProt ID

    P14679

  • Gene ID
    7299 [NCBI]
  • Synonyms

    ATN; CMM8; LB24 AB; SHEP3; SK29 AB; Tumor rejection antigen AB; TYR

  • Research Field

    Tags & Cell Markers

Tyrosinase Antibody (YA2522) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling Tyrosinase Antibody (HY-P82777) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with Tyrosinase Antibody (HY-P82777) at 1/50 dilution in BSA for Immunol Staining at 4 ℃ Overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling Tyrosinase Antibody (HY-P82777) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with Tyrosinase Antibody (HY-P82777) at 1/200dilution in BSA for Immunol Staining at 4 ℃ Overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Tyrosinase Antibody (YA2522) Product Image
    1/16
  • Experimental Validation Results for Tyrosinase Antibody (YA2522)
  • Experimental Validation Results for Tyrosinase Antibody (YA2522)
  • Tyrosinase Antibody (YA2522) Product Image
  • Experimental Validation Results for Tyrosinase Antibody (YA2522)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling Tyrosinase Antibody (HY-P82777) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with Tyrosinase Antibody (HY-P82777) at 1/50 dilution in BSA for Immunol Staining at 4 ℃ Overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Tyrosinase Antibody (YA2522)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling Tyrosinase Antibody (HY-P82777) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with Tyrosinase Antibody (HY-P82777) at 1/200dilution in BSA for Immunol Staining at 4 ℃ Overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Tyrosinase Antibody (YA2522)
  • Experimental Validation Results for Tyrosinase Antibody (YA2522)
  • Tyrosinase Antibody (YA2522) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of Hela cells labeling Tyrosinase Antibody (HY-P82777) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with Tyrosinase Antibody (HY-P82777) at 1/50 dilution in BSA for Immunol Staining at 4 ℃ Overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Tyrosinase Antibody (YA2522) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of Hela cells labeling Tyrosinase Antibody (HY-P82777) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with Tyrosinase Antibody (HY-P82777) at 1/200dilution in BSA for Immunol Staining at 4 ℃ Overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Tyrosinase Antibody (YA2522) Product Image ICC
  • Tyrosinase Antibody (YA2522) Product Image ICC