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FOXP3 Antibody (YA425)

(Synonyms: IPEX, JM2, FOXP3, Forkhead box protein P3, Scurfin)
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Based on 1 Customer Validation

FOXP3 Antibody (YA425) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to FOXP3.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, ICC/IF, FC

  • Reactivity :

    Human

  • Formulation:

    Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for FOXP3 Antibody (YA425)
  • FOXP3 Antibody (YA425) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Applications

Application
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
FC Info
FC: Flow Cytometry
Dilution Ratio 1:500-1:2000 1:50-1:100 1:50-1:100

Product Details

Description

FOXP3 Antibody (YA425) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to FOXP3.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 47 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 47 kDa
Species Reactivity Database

Entrez Gene: 50943 Human

SwissProt: Q9BZS1 Human

Immunogen

Synthetic peptide corresponding to Human FOXP3.AA range:250-431.

Sensitivity

Endogenous

Purification

Protein A affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3102315

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for FOXP3 Antibody (YA425)
    Western blot analysis of extracts from K562 (lane 1) and Raji (lane 2) using FOXP3 antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (GAPDH, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.

Background

  • Function

    FOXP3, so called scurfin, belongs to the forkhead box protein P (FOXP) family. FOXP3 is critically involved in differentiation and function of regulatory T cells or CD4+/CD25+ regulatory T (Treg) cells for cancer immunotherapy. FOXP3 modulates Treg development and functions by immune evasion of tumor cells through imbalance of immunoediting and immunosurveillance in some cancers. FOXP3 is associated with IL-17, RUNX1, STAT3, NF-κB, FOXO3 and other cofactors such as EOS (Ikzf4)， interferon regulatory factor 4 (IRF4)， special AT-rich sequence-binding protein-1 (SATB1) and GATA1[1][2].

  • Subcellular Localization

    Nucleus; Cytoplasm

  • Expression


    Induction:Down-regulated in regulatory T-cells (Treg) during inflammation (PubMed:23973223) . Up-regulated by FOXO3 (PubMed:30513302)

  • Isoforms & Post-Translational Modification

    Q9BZS1 has 4 isomers: Q9BZS1-1: 47244 Da (predicted); Q9BZS1-2: 43410 Da (predicted); Q9BZS1-3: 49843 Da (predicted); Q9BZS1-4: 44407 Da (predicted).
    Polyubiquitinated, leading to its proteasomal degradation in regulatory T-cells (Treg) which is mediated by STUB1 in a HSPA1A/B-dependent manner. Deubiquitinated by USP7 and USP44; leading to increase in protein stability;Phosphorylation at Ser-418 regulates its transcriptional repressor activity and consequently, regulatory T-cells (Treg) suppressive function. Dephosphorylated at Ser-418 by protein phosphatase 1 (PP1) in Treg cells derived from patients with rheumatoid arthritis. Phosphorylation by CDK2 negatively regulates its transcriptional activity and protein stability (By similarity);Acetylation on lysine residues stabilizes FOXP3 and promotes differentiation of T-cells into induced regulatory T-cells (iTregs) associated with suppressive functions (PubMed:17360565, PubMed:24835996). Acetylation is mediated by a coordinated action of KAT5 and EP300/p300 acetyltransferases: EP300/p300 is required to enhance KAT5 autoacetylation, promoting acetylation of FOXP3 by KAT5 (PubMed:24835996). Deacetylated by SIRT1 (PubMed:22312127);Undergoes proteolytic cleavage in activated regulatory T-cells (Treg), and can be cleaved at either the N- or C-terminal site, or at both sites

  • Subunit

    Homodimer (PubMed:21458306, PubMed:25567984). Dimerization is essential for its transcriptional regulator activity (PubMed:21458306). Interacts with IKZF3. Isoform 1 (via LXXLL motif), but not isoform 2, interacts with isoform 4 of RORA (via AF-2 motif). Interacts with STUB1, HSPA8 and HSPA1A/B. Interacts with PPP1CA, PPP1CB and PPP1CG. Interacts with KAT5 and HDAC7. Interacts with HDAC9 in the absence of T-cell stimulation. Interacts with USP7. Interacts with isoform 2 of ZFP90 and can form a complex with TRIM28 in the presence of isoform 2 of ZFP90. Interacts with RUNX1. Interacts with RORC. Interacts with RELA and NFATC2. Interacts with RUNX2, RUNX3 and IKZF4 (By similarity)

  • SwissProt ID

    Q9BZS1

  • Gene ID
    50943 [NCBI]
  • Synonyms

    IPEX, JM2, FOXP3, Forkhead box protein P3, Scurfin

  • Research Field

    Cell Biology

[1]. Kim JH, et al. Molecular networks of FOXP family: dual biologic functions, interplay with other molecules and clinical implications in cancer progression. Mol Cancer. 2019 Dec 9;18(1):180. [Content Brief]

[2]. Co M, et al. FOXP transcription factors in vertebrate brain development, function, and disorders. Wiley Interdiscip Rev Dev Biol. 2020 Sep;9(5):e375. [Content Brief]

FOXP3 Antibody (YA425) Related Classifications

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100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis of extracts from K562 (lane 1) and Raji (lane 2) using FOXP3 antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (GAPDH, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.

  • FOXP3 Antibody (YA425) Product Image
    1/16
  • Experimental Validation Results for FOXP3 Antibody (YA425)
  • FOXP3 Antibody (YA425) Product Image
  • Experimental Validation Results for FOXP3 Antibody (YA425)
    1/1
    MedchemExpress Validation
     Western blot analysis of extracts from K562 (lane 1) and Raji (lane 2) using FOXP3 antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (GAPDH, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.
  • Experimental Validation Results for FOXP3 Antibody (YA425)
  • FOXP3 Antibody (YA425) Product Image
    MedchemExpress Validation

    Western blot analysis of extracts from K562 (lane 1) and Raji (lane 2) using FOXP3 antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (GAPDH, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.

  • FOXP3 Antibody (YA425) Product Image WB