PRC1 Antibody (YA1716)

Cat. No.: HY-P81971: Data Sheet COA
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User Guide for Antibodies
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PRC1 Antibody (YA1716) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to PRC1.

For research use only. We do not sell to patients.

Size	Stock	Quantity
Free Sample	Apply now
10 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
250 μL	Get quote

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PRC1 Antibody (YA1716) Featured Recommendations:

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
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Background
Documentation

Description

PRC1 Antibody (YA1716) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to PRC1.

Host

Rabbit

Clonality

Recombinant,Monoclonal

Molecular Weight

Predicted band size: 72 kDa;

Observed band size: 72 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Rat

SwissProt ID

O43663

Gene ID

9055 [NCBI]

Immunogen

A synthetic peptide of human PRC1

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:50-1:100
IP IP: Immunoprecipitation	1:20

Sensitivity	Endogenous	Purity	Affinity Purified
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Verification Image

Western blot analysis was performed on extracts from Jurkat (lane 1, 15 μg), Hela (lane 2, 15 μg), C2C12 (lane 3, 15 μg), Raji (lane 4, 15 μg), HCT116 (lane 5, 15 μg), and C6 (lane 6, 15 μg) using PRC1 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (GAPDH, HY-P80137, 1:60000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

Background

Function：Key regulator of cytokinesis that cross-links antiparrallel microtubules at an average distance of 35 nM. Essential for controlling the spatiotemporal formation of the midzone and successful cytokinesis. Required for KIF14 localization to the central spindle and midbody. Required to recruit PLK1 to the spindle. Stimulates PLK1 phosphorylation of RACGAP1 to allow recruitment of ECT2 to the central spindle. Acts as an oncogene for promoting bladder cancer cells proliferation, apoptosis inhibition and carcinogenic progression (PubMed:17409436)

Subcellular Localization：Nucleus; Cytoplasm; Cytoplasm, cytoskeleton, spindle pole; Midbody; Chromosome

Expression：
Tissue_specificity:Overexpression in bladder cancer cells (PubMed: 17409436)

Isoforms & Post-Translational Modification：O43663 has 4 isomers: O43663-1: 71607 Da (predicted); O43663-2: 66596 Da (predicted); O43663-3: 61388 Da (predicted); O43663-4: 70191 Da (predicted).
Phosphorylation by CDK1 in early mitosis holds PRC1 in an inactive monomeric state, during the metaphase to anaphase transition, PRC1 is dephosphorylated, promoting interaction with KIF4A, which then translocates PRC1 along mitotic spindles to the plus ends of antiparallel interdigitating microtubules. Dephosphorylation also promotes MT-bundling activity by allowing dimerization. Phosphorylation by CDK1 prevents PLK1-binding: upon degradation of CDK1 at anaphase and dephosphorylation, it is then phosphorylated by PLK1, leading to cytokinesis

Subunit：Homodimer (PubMed:20691902). Interacts with the C-terminal Rho-GAP domain and the basic region of RACGAP1 (PubMed:14744859). The interaction with RACGAP1 inhibits its GAP activity towards CDC42 in vitro, which may be required for maintaining normal spindle morphology (PubMed:14744859). Interacts (via N-terminus) with the C-terminus of CENPE (via C-terminus); the interaction occurs during late mitosis (PubMed:15297875). Interacts (via N-terminus) with KIF4A (via C-terminus); the interaction is required for the progression of mitosis (PubMed:15297875, PubMed:16431929, PubMed:29848660). Interacts (via N-terminus) with KIF23 (via C-terminus); the interaction occurs during late mitosis (PubMed:15297875). Interacts with KIF14 and KIF20A (PubMed:15625105, PubMed:16431929). Interacts with PLK1 (PubMed:19468300). Interacts with KIF20B (PubMed:17409436). Interacts with CCDC66 (PubMed:35849559)

RRID

AB_3104092

Database

Entrez Gene: 9055 Human ; 308761 Rat

SwissProt: O43663 Human ;

OMIM: 603484 Human

Research Field

Cell Biology

Synonyms

Protein regulator of cytokinesis 1

Documentation

Select Batch:

Data Sheet (234 KB) SDS (251 KB)

COA (205 KB)

User Guide for Antibodies (1077 KB)

PRC1 Antibody (YA1716) Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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PRC1 Antibody (YA1716)

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Background

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PRC1 Antibody (YA1716) Related Classifications

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