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  4. Monoclonal Antibodies Recombinant Antibodies
  5. alpha Sarcoglycan Antibody (YA2068)

alpha Sarcoglycan Antibody (YA2068)

Cat. No.: HY-P82323
COA
SDS
User Guide for Antibodies Technical Support

alpha Sarcoglycan Antibody (YA2068) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to alpha Sarcoglycan.

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Top Publications Citing Use of Products
  • WB: Western Blot;
  • IHC-P: Immunohistochemistry-Paraffin;
  • IHC-F: Immunohistochemistry-Frozen;
  • ICC/IF: Immunocytochemistry/Immunofluorescence;
  • IF-Tissue: Immunofluorescence-Tissue;
  • mIHC: Multiplex Immunohistochemical;
  • IP: Immunoprecipitation;
  • ChIP: Chromatin Immunoprecipitation;
  • FC: Flow Cytometry;
  • ELISA: Enzyme Linked Immunosorbent Assay

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  • Description

Description

alpha Sarcoglycan Antibody (YA2068) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to alpha Sarcoglycan.
Host

Rabbit
Clonality

Recombinant,Monoclonal
Masse moléculaire
Predicted band size: 43 kDa;
Observed band size: 50 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
Species Reactivity
Human, Mouse, Rat
SwissProt ID
Gene ID
Immunogen

A synthetic peptide of human alpha Sarcoglycan
Application &
Dilution Ratio
Application Dilution Ratio
WB
WB: Western Blot
1:500-1:1000
IHC-P
IHC-P: Immunohistochemistry-Paraffin
1:50-1:100
IP
IP: Immunoprecipitation
1:20
Sensitivity Endogenous Pureté Affinity Purified
Conjugation Non-conjugated Modification Unmodified
Isotype IgG  
Appearance

Liquid
Formulation

Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
Livraison

Shipping with blue ice.
Verification Image
ALL WB IHC-P

  • Western blot analysis was performed on extracts from Mouse skeletal muscle (lane 1, 15 μg), Rat skeletal muscle (lane 2, 15 μg), Mouse heart (lane 3, 15 μg), and Rat heart (lane 4, 15 μg) using alpha Sarcoglycan Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (GAPDH, HY-P80137, 1:90000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • Western blot analysis of extracts from C6 (lane 2(20μg) and C6 (lane 3(40μg) using alpha Sarcoglycan (HY-P82323) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded Mouse heart tissue using alpha Sarcoglycan Antibody (YA2068). The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P82323, 1/100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded Mouse heart tissue using alpha Sarcoglycan Antibody (YA2068). The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P82323, 1/100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Background
Function：Component of the sarcoglycan complex, a subcomplex of the dystrophin-glycoprotein complex which forms a link between the F-actin cytoskeleton and the extracellular matrix
Subcellular Localization：Cell membrane, sarcolemma; Single-pass type I membrane protein; Cytoplasm, cytoskeleton
Expression：
Tissue_specificity:Most strongly expressed in skeletal muscle. Also expressed in cardiac muscle and, at much lower levels, in lung. In the fetus, most abundant in cardiac muscle and, at lower levels, in lung. Also detected in liver and kidney. Not expressed in brain
Isoforms & Post-Translational Modification：Q16586 has 2 isomers: Q16586-1: 42875 Da (predicted); Q16586-2: 29354 Da (predicted).
Subunit：Interacts with the syntrophin SNTA1. Cross-link to form 2 major subcomplexes: one consisting of SGCB, SGCD and SGCG and the other consisting of SGCB and SGCD.
RRID
Database

Entrez Gene: 6442 Human ; 20391 Mouse ; 303468 Rat

SwissProt: Q16586 Human ; P82350 Mouse ; Q5SWB2 Mouse ;

OMIM: 608099 Human

Research Field

Signal Transduction
Synonyms
SGCA; ADL; DAG2; Alpha-sarcoglycan; Alpha-SG; 50 kDa dystrophin-associated glycoprotein; 50DAG; Adhalin; Dystroglycan-2
Documentation
SDS (251 KB)

COA (206 KB)

User Guide for Antibodies (1077 KB)

alpha Sarcoglycan Antibody (YA2068) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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alpha Sarcoglycan Antibody (YA2068)
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HY-P82323
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