NKRF Antibody (YA8844)

Cat. No.: HY-P89160: Data Sheet User Guide for Antibodies
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NKRF Antibody (YA8844) is a Mouse-derived and non-conjugated IgG2a monoclonal antibody, targeting to NKRF.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

NKRF Antibody (YA8844) is a Mouse-derived and non-conjugated IgG2a monoclonal antibody, targeting to NKRF.

Host

Mouse

Clonality

Monoclonal

Species Reactivity

Human, Mouse

SwissProt ID

O15226

Immunogen

Human recombinant protein fragment corresponding to amino acids 1-296 of human NKRF produced in E.coli.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-2000

Sensitivity	Endogenous	Purity	Affinity purified
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG2a

Appearance

Liquid

Formulation

Spplied in PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Enhances the ATPase activity of DHX15 by acting like a brace that tethers mobile sections of DHX15 together, stabilizing a functional conformation with high RNA affinity of DHX15 (PubMed:12381793). Involved in the constitutive silencing of the interferon beta promoter, independently of the virus-induced signals, and in the inhibition of the basal and cytokine-induced iNOS promoter activity (PubMed:12381793). Also involved in the regulation of IL-8 transcription (PubMed:12381793). May also act as a DNA-binding transcription regulator: interacts with a specific negative regulatory element (NRE) 5'-AATTCCTCTGA-3' to mediate transcriptional repression of certain NK-kappa-B responsive genes (PubMed:10562553)

Subcellular Localization：Nucleus, nucleolus

Expression：
Tissue_Specificity: Widely and constitutively expressed (PubMed:10562553). Expressed at lower level in colon, peripheral blood lymphocytes, lung and kidney (PubMed:10562553)

Isoforms & Post-Translational Modification：O15226 has two isomers: O15226-1: 77673 Da (predicted); O15226-2: 79496 Da (predicted).

Subunit：Interacts with NF-kappa-B (PubMed:10562553)

Synonyms

ITBA4; NRF

Documentation

User Guide for Antibodies (1077 KB)