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AFP Antibody (YA914)

(Synonyms: Alpha Fetoprotein; Alpha-fetoprotein; alpha 1 fetoprotein; Alpha-1-fetoprotein; Alpha-fetoglobulin; alpha fetoglobulin; alpha Fetoprotein; Alpha fetoprotein precursor; FETA; HPAFP; FETA_HUMAN.)
Customer Review

Based on 1 Customer Validation

AFP Antibody (YA914) is a Mouse-derived and non-conjugated IgG monoclonal antibody, targeting to AFP.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG

  • Application:

    WB, ELISA, IHC-P, IHC-F, ICC/IF

  • Reactivity :

    Human, Rat,

  • Formulation:

    Supplied in 0.01M PBS(pH7.4) with 0.03% Proclin300.

  • Conjugation:
    Non-conjugated
ALL WB ICC
  • Experimental Validation Results for AFP Antibody (YA914)
  • Experimental Validation Results for AFP Antibody (YA914)
  • Experimental Validation Results for AFP Antibody (YA914)
  • AFP Antibody (YA914) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
IHC-F Info
IHC-F: Immunohistochemistry-Frozen
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
Dilution Ratio 1:500-2000 1:5000-10000 1:100-500 1:100-500 1:100-500

Product Details

Description

AFP Antibody (YA914) is a Mouse-derived and non-conjugated IgG monoclonal antibody, targeting to AFP.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human, Rat,
  • Observed Molecular Weight
    Observed band size: 72 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 65 kDa
Species Reactivity Database
Immunogen

AFP protein: full length

Sensitivity

Endogenous

Purification

affinity purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3103052

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 0.01M PBS(pH7.4) with 0.03% Proclin300.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for AFP Antibody (YA914)
    Western blot analysis of extracts from HepG2 (lane 1) using AFP antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/2000) and Loading control antibody (GAPDH, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-mouse IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.
  • Experimental Validation Results for AFP Antibody (YA914)
    Immunocytochemistry analysis of MCF-7 cells labeling AFP with AFP Antibody (HY-P81077) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with AFP Antibody (HY-P81077) at 1/100 dilution in quick block buffer overnight at 4 ℃.AF488-conjugated Goat Anti-Mouse IgG H&L (HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for AFP Antibody (YA914)
    Immunocytochemistry analysis of MCF-7 cells labeling AFP with AFP Antibody (HY-P81077) at 1/300 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with AFP Antibody (HY-P81077) at 1/300 dilution in quick block buffer overnight at 4 ℃.AF488-conjugated Goat Anti-Mouse IgG H&L (HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

  • Function

    AFP binds copper, nickel, and fatty acids as well as, and bilirubin less well than, serum albumin. Only a small percentage (less than 2%) of the human AFP shows estrogen-binding properties

  • Subcellular Localization

    Secreted

  • Expression


    Tissue_specificity:Blood plasma. Synthesized from fetal liver and yolk sac.

  • Subunit

    Dimeric and trimeric forms have been found in addition to the monomeric form

  • SwissProt ID

    P02771

  • Gene ID
    174 [NCBI]
  • Synonyms

    Alpha Fetoprotein; Alpha-fetoprotein; alpha 1 fetoprotein; Alpha-1-fetoprotein; Alpha-fetoglobulin; alpha fetoglobulin; alpha Fetoprotein; Alpha fetoprotein precursor; FETA; HPAFP; FETA_HUMAN.

AFP Antibody (YA914) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis of extracts from HepG2 (lane 1) using AFP antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/2000) and Loading control antibody (GAPDH, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-mouse IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of MCF-7 cells labeling AFP with AFP Antibody (HY-P81077) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with AFP Antibody (HY-P81077) at 1/100 dilution in quick block buffer overnight at 4 ℃.AF488-conjugated Goat Anti-Mouse IgG H&L (HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of MCF-7 cells labeling AFP with AFP Antibody (HY-P81077) at 1/300 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with AFP Antibody (HY-P81077) at 1/300 dilution in quick block buffer overnight at 4 ℃.AF488-conjugated Goat Anti-Mouse IgG H&L (HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • AFP Antibody (YA914) Product Image
    1/16
  • Experimental Validation Results for AFP Antibody (YA914)
  • Experimental Validation Results for AFP Antibody (YA914)
  • Experimental Validation Results for AFP Antibody (YA914)
  • AFP Antibody (YA914) Product Image
  • Experimental Validation Results for AFP Antibody (YA914)
    1/1
    MedchemExpress Validation
     Western blot analysis of extracts from HepG2 (lane 1) using AFP antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/2000) and Loading control antibody (GAPDH, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-mouse IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.
  • Experimental Validation Results for AFP Antibody (YA914)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of MCF-7 cells labeling AFP with AFP Antibody (HY-P81077) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with AFP Antibody (HY-P81077) at 1/100 dilution in quick block buffer overnight at 4 ℃.AF488-conjugated Goat Anti-Mouse IgG H&L (HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for AFP Antibody (YA914)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of MCF-7 cells labeling AFP with AFP Antibody (HY-P81077) at 1/300 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with AFP Antibody (HY-P81077) at 1/300 dilution in quick block buffer overnight at 4 ℃.AF488-conjugated Goat Anti-Mouse IgG H&L (HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for AFP Antibody (YA914)
  • Experimental Validation Results for AFP Antibody (YA914)
  • Experimental Validation Results for AFP Antibody (YA914)
  • AFP Antibody (YA914) Product Image
    MedchemExpress Validation

    Western blot analysis of extracts from HepG2 (lane 1) using AFP antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/2000) and Loading control antibody (GAPDH, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-mouse IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.

  • AFP Antibody (YA914) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of MCF-7 cells labeling AFP with AFP Antibody (HY-P81077) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with AFP Antibody (HY-P81077) at 1/100 dilution in quick block buffer overnight at 4 ℃.AF488-conjugated Goat Anti-Mouse IgG H&L (HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • AFP Antibody (YA914) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of MCF-7 cells labeling AFP with AFP Antibody (HY-P81077) at 1/300 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with AFP Antibody (HY-P81077) at 1/300 dilution in quick block buffer overnight at 4 ℃.AF488-conjugated Goat Anti-Mouse IgG H&L (HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • AFP Antibody (YA914) Product Image WB
  • AFP Antibody (YA914) Product Image ICC
  • AFP Antibody (YA914) Product Image ICC