Ubiquitin Protein Ligase E3A Antibody (YA3306)
(Synonyms: Ubiquitin-protein ligase E3A; UBE3A; E6AP; EPVE6AP; HPVE6A)
Based on 1 Customer Validation
Ubiquitin Protein Ligase E3A Antibody (YA3306) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Ubiquitin Protein Ligase E3A.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, ICC/IF, FC, IP
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Reactivity :
Human, Mouse, Rat
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Formulation:
Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
IP
IP: Immunoprecipitation
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|---|---|---|---|
| Dilution Ratio | 1:500-1:1000 | 1:50-1:200 | 1:20 |
Product Details
Ubiquitin Protein Ligase E3A Antibody (YA3306) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Ubiquitin Protein Ligase E3A.
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Host Rabbit
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Clonality Recombinant,Monoclonal
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Species ReactivityHuman, Mouse, Rat
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Observed Molecular WeightObserved band size: 101 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 101 kDa
Entrez Gene: 7337 Human ; 22215 Mouse ; 361585 Rat
SwissProt: Q05086 Human ; O08759 Mouse ;
OMIM: 105830 Human
Recombinant protein of human UBE3A
Endogenous
Affinity Purified
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
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Concentration
Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
Ubiquitin Protein Ligase E3A is an E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and transfers it to its substrates. Several substrates have been identified including the BMAL1, ARC, LAMTOR1, RAD23A and RAD23B, MCM7 (which is involved in DNA replication), annexin A1, the PML tumor suppressor, and the cell cycle regulator CDKN1B. Additionally, may function as a cellular quality control ubiquitin ligase by helping the degradation of the cytoplasmic misfolded proteins. Finally, UBE3A also promotes its own degradation in vivo. Plays an important role in the regulation of the circadian clock: involved in the ubiquitination of the core clock component BMAL1, leading to its proteasomal degradation. Acts as transcriptional coactivator of progesterone receptor PGR upon progesterone hormone activation. Acts as a regulator of synaptic development by mediating ubiquitination and degradation of ARC. Required for synaptic remodeling in neurons by mediating ubiquitination and degradation of LAMTOR1, thereby limiting mTORC1 signaling and activity-dependent synaptic remodeling. Synergizes with WBP2 in enhancing PGR activity; (Microbial infection) Catalyzes the high-risk human papilloma virus E6-mediated ubiquitination of p53/TP53, contributing to the neoplastic progression of cells infected by these viruses[1][2][3][4][5][6][7][8][9][10].
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Subcellular Localization
Cytoplasm; Nucleus
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Isoforms & Post-Translational Modification
Q05086 has 3 isomers: Q05086-1: 100688 Da (predicted); Q05086-2: 97968 Da (predicted); Q05086-3: 100102 Da (predicted).
Phosphorylation at Tyr-659 by ABL1 impairs E3 ligase activity and protects p53/TP53 from degradation in (HPV)-infected cells -
Subunit
The active form is probably a homotrimer. Binds UBQLN1 and UBQLN2. Interacts with the 26S proteasome. Interacts with BPY2. Interacts with HIF1AN, MAPK6 and NEURL4; interaction with MAPK6 may be mediated by NEURL4.
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SwissProt ID
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Synonyms
Ubiquitin-protein ligase E3A; UBE3A; E6AP; EPVE6AP; HPVE6A
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Research Field
Epigenetics and Nuclear Signaling
Documentation
References
[1]. Kumar S, et al. Identification of HHR23A as a substrate for E6-associated protein-mediated ubiquitination. J Biol Chem. 1999 Jun 25;274(26):18785-92. [Content Brief]
[2]. Dhananjayan SC, et al. WW domain binding protein-2, an E6-associated protein interacting protein, acts as a coactivator of estrogen and progesterone receptors. Mol Endocrinol. 2006 Oct;20(10):2343-54. [Content Brief]
[3]. Shimoji T, et al. Identification of annexin A1 as a novel substrate for E6AP-mediated ubiquitylation. J Cell Biochem. 2009 Apr 15;106(6):1123-35. [Content Brief]
[4]. Mishra A, et al. The ubiquitin ligase E6-AP is induced and recruited to aggresomes in response to proteasome inhibition and may be involved in the ubiquitination of Hsp70-bound misfolded proteins. J Biol Chem. 2009 Apr 17;284(16):10537-45. [Content Brief]
[5]. Louria-Hayon I, et al. E6AP promotes the degradation of the PML tumor suppressor. Cell Death Differ. 2009 Aug;16(8):1156-66. [Content Brief]
[6]. Mishra A, et al. UBE3A/E6-AP regulates cell proliferation by promoting proteasomal degradation of p27. Neurobiol Dis. 2009 Oct;36(1):26-34. [Content Brief]
[7]. Martínez-Noël G, et al. Identification and proteomic analysis of distinct UBE3A/E6AP protein complexes. Mol Cell Biol. 2012 Aug;32(15):3095-106. [Content Brief]
[8]. Ronchi VP, et al. The active form of E6-associated protein (E6AP)/UBE3A ubiquitin ligase is an oligomer. J Biol Chem. 2014 Jan 10;289(2):1033-48. [Content Brief]
[9]. Gossan NC, et al. The E3 ubiquitin ligase UBE3A is an integral component of the molecular circadian clock through regulating the BMAL1 transcription factor. Nucleic Acids Res. 2014 May;42(9):5765-75. [Content Brief]
[10]. Sun J, et al. UBE3A-mediated p18/LAMTOR1 ubiquitination and degradation regulate mTORC1 activity and synaptic plasticity. Elife. 2018 Jul 18;7:. [Content Brief]
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