Topoisomerase I Antibody (YA3758)
(Synonyms: TOPI)Topoisomerase I Antibody (YA3758) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to Topoisomerase I.
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Host:
Mouse
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Isotype:
IgG1
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Application:
WB, ICC/IF, FC
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Reactivity :
Human, Mouse
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Formulation:
Supplied in PBS with 0.05% sodium azide
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Conjugation:
Non-conjugated
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Applications
| Application |
WB
WB: Western Blot
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ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
FC
FC: Flow Cytometry
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|---|---|---|---|
| Dilution Ratio | 1:500-1:2000 | 1:100-1:400 | 1:200-1:400 |
Product Details
Topoisomerase I Antibody (YA3758) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to Topoisomerase I.
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Host Mouse
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Clonality Monoclonal
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Species ReactivityHuman, Mouse
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Observed Molecular WeightObserved band size: 91 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 91 kDa
Purified recombinant fragment of human TOP1 aa 2-150.
affinity purified.
Non-conjugated
Unmodified
IgG1
Product Properties
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Appearance
Liquid
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Formulation
Supplied in PBS with 0.05% sodium azide
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
Topoisomerase I is a Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand then rotates around the intact phosphodiester bond on the opposing strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone. Regulates the alternative splicing of tissue factor (F3) pre-mRNA in endothelial cells. Involved in the circadian transcription of the core circadian clock component BMAL1 by altering the chromatin structure around the ROR response elements (ROREs) on the BMAL1 promoter
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Subcellular Localization
Nucleus, nucleolus; Nucleus, nucleoplasm
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Expression
Tissue_specificity:Endothelial cells -
Subunit
Monomer. Interacts with ERCC6 (PubMed:26030138). Interacts with TPRN; TPRN interacts with a number of DNA damage response proteins, is recruited to sites of DNA damage and may play a role in DNA damage repair (PubMed:23213405)
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SwissProt ID
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Synonyms
TOPI
Documentation
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