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SHARP2 Antibody (YA6764)

(Synonyms: BHE40_HUMAN antibody; bHLHB2 antibody; bHLHe40 antibody; Class B Basic Helix Loop Helix Protein 2 antibody; Class B basic helix-loop-helix protein 2 antibody; Class E basic helix loop helix protein 40 antibody; Class E basic helix-loop-helix protein 40 antibody; Clast5 antibody; DEC1 antibody; Differentially expressed in chondrocytes protein 1 antibody; BHE40_HUMAN antibody; bHLHB2 antibody; bHLHe40 antibody; Class B Basic Helix Loop Helix Protein 2 antibody; Class B basic helix-loop-helix protein 2 antibody; Class E basic helix loop helix protein 40 antibody; Class E basic helix-loop-helix protein 40 antibody; Clast5 antibody; DEC1 antibody; Differentially expressed in chondrocytes protein 1 antibody; E47 interaction protein 1 antibody; EIP1 antibody; Enhancer of split and hairy related protein 2 antibody; Enhancer-of-split and hairy-related protein 2 antibody; SHARP 2 antibody; SHARP-2 antibody; Stimulated by retinoic acid gene 13 protein antibody; STRA13 antibody; )
Customer Review

Based on 1 Customer Validation

SHARP2 Antibody (YA6764) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to SHARP2.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, ICC/IF

  • Reactivity :

    Human

  • Formulation:

    Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Conjugation:
    Non-conjugated
ALL WB IHC-P ICC
  • Experimental Validation Results for SHARP2 Antibody (YA6764)
  • Experimental Validation Results for SHARP2 Antibody (YA6764)
  • Experimental Validation Results for SHARP2 Antibody (YA6764)
  • Experimental Validation Results for SHARP2 Antibody (YA6764)
  • Experimental Validation Results for SHARP2 Antibody (YA6764)
  • Experimental Validation Results for SHARP2 Antibody (YA6764)
  • Experimental Validation Results for SHARP2 Antibody (YA6764)
  • SHARP2 Antibody (YA6764) Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
Dilution Ratio 1:1000 1:1000 1:100

Product Details

Description

SHARP2 Antibody (YA6764) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to SHARP2.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 46/50 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 46 kDa
Immunogen

Recombinant protein within human SHARP2 aa 151-412 / 412.

Purification

affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for SHARP2 Antibody (YA6764)
    Western blot analysis of extracts from Hela(lane2(20μg), MDA-MB-231(lane3(20μg), 293T(lane4(20μg) and A431(lane5(20μg) using SHARP2 Antibody (YA6764)(HY-P87071). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for SHARP2 Antibody (YA6764)
    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue using SHARP2 Antibody (HY-P87071, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for SHARP2 Antibody (YA6764)
    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using SHARP2 Antibody (HY-P87071, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for SHARP2 Antibody (YA6764)
    Immunohistochemical analysis of paraffin-embedded human esophageal cancer tissue using SHARP2 Antibody (HY-P87071, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for SHARP2 Antibody (YA6764)
    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using SHARP2 Antibody (HY-P87071, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for SHARP2 Antibody (YA6764)
    Immunocytochemistry analysis of MCF-7 cells labeling SHARP2 with SHARP2 Antibody (HY-P87071) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with SHARP2 Antibody (HY-P87071) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for SHARP2 Antibody (YA6764)
    Immunocytochemistry analysis of U2OS cells labeling SHARP2 with SHARP2 Antibody (HY-P87071) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with SHARP2 Antibody (HY-P87071) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

  • Function

    Transcriptional repressor involved in the regulation of the circadian rhythm by negatively regulating the activity of the clock genes and clock-controlled genes (PubMed:12397359, PubMed:18411297). Acts as the negative limb of a novel autoregulatory feedback loop (DEC loop) which differs from the one formed by the PER and CRY transcriptional repressors (PER/CRY loop) (PubMed:14672706). Both these loops are interlocked as it represses the expression of PER1/2 and in turn is repressed by PER1/2 and CRY1/2 (PubMed:15193144). Represses the activity of the circadian transcriptional activator: CLOCK-BMAL1|BMAL2 heterodimer by competing for the binding to E-box elements (5'-CACGTG-3') found within the promoters of its target genes (PubMed:15560782). Negatively regulates its own expression and the expression of DBP and BHLHE41/DEC2 (PubMed:14672706). Acts as a corepressor of RXR and the RXR-LXR heterodimers and represses the ligand-induced RXRA and NR1H3/LXRA transactivation activity (PubMed:19786558). May be involved in the regulation of chondrocyte differentiation via the cAMP pathway (PubMed:19786558). Represses the transcription of NR0B2 and attentuates the transactivation of NR0B2 by the CLOCK-BMAL1 complex (PubMed:28797635). Drives the circadian rhythm of blood pressure through transcriptional repression of ATP1B1 in the cardiovascular system (PubMed:30012868)

  • Subcellular Localization

    Cytoplasm,Nucleus

  • Expression


    Tissue_Specificity: Expressed in cartilage, spleen, intestine, lung, and to a lesser extent in heart, brain, liver, muscle and stomach

  • Isoforms & Post-Translational Modification

    O14503: 412 amino acids, molecular weight 45510 Da.

  • Subunit

    Homodimer

  • SwissProt ID

    O14503

  • Gene ID
    8553 [NCBI]
  • Synonyms

    BHE40_HUMAN antibody; bHLHB2 antibody; bHLHe40 antibody; Class B Basic Helix Loop Helix Protein 2 antibody; Class B basic helix-loop-helix protein 2 antibody; Class E basic helix loop helix protein 40 antibody; Class E basic helix-loop-helix protein 40 antibody; Clast5 antibody; DEC1 antibody; Differentially expressed in chondrocytes protein 1 antibody; BHE40_HUMAN antibody; bHLHB2 antibody; bHLHe40 antibody; Class B Basic Helix Loop Helix Protein 2 antibody; Class B basic helix-loop-helix protein 2 antibody; Class E basic helix loop helix protein 40 antibody; Class E basic helix-loop-helix protein 40 antibody; Clast5 antibody; DEC1 antibody; Differentially expressed in chondrocytes protein 1 antibody; E47 interaction protein 1 antibody; EIP1 antibody; Enhancer of split and hairy related protein 2 antibody; Enhancer-of-split and hairy-related protein 2 antibody; SHARP 2 antibody; SHARP-2 antibody; Stimulated by retinoic acid gene 13 protein antibody; STRA13 antibody;

SHARP2 Antibody (YA6764) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from Hela(lane2(20μg), MDA-MB-231(lane3(20μg), 293T(lane4(20μg) and A431(lane5(20μg) using SHARP2 Antibody (YA6764)(HY-P87071). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue using SHARP2 Antibody (HY-P87071, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using SHARP2 Antibody (HY-P87071, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human esophageal cancer tissue using SHARP2 Antibody (HY-P87071, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using SHARP2 Antibody (HY-P87071, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of MCF-7 cells labeling SHARP2 with SHARP2 Antibody (HY-P87071) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with SHARP2 Antibody (HY-P87071) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of U2OS cells labeling SHARP2 with SHARP2 Antibody (HY-P87071) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with SHARP2 Antibody (HY-P87071) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • SHARP2 Antibody (YA6764) Product Image
    1/16
  • Experimental Validation Results for SHARP2 Antibody (YA6764)
  • Experimental Validation Results for SHARP2 Antibody (YA6764)
  • Experimental Validation Results for SHARP2 Antibody (YA6764)
  • Experimental Validation Results for SHARP2 Antibody (YA6764)
  • Experimental Validation Results for SHARP2 Antibody (YA6764)
  • Experimental Validation Results for SHARP2 Antibody (YA6764)
  • Experimental Validation Results for SHARP2 Antibody (YA6764)
  • SHARP2 Antibody (YA6764) Product Image
  • Experimental Validation Results for SHARP2 Antibody (YA6764)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from Hela(lane2(20μg), MDA-MB-231(lane3(20μg), 293T(lane4(20μg) and A431(lane5(20μg) using SHARP2 Antibody (YA6764)(HY-P87071). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for SHARP2 Antibody (YA6764)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue using SHARP2 Antibody (HY-P87071, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for SHARP2 Antibody (YA6764)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using SHARP2 Antibody (HY-P87071, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for SHARP2 Antibody (YA6764)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human esophageal cancer tissue using SHARP2 Antibody (HY-P87071, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for SHARP2 Antibody (YA6764)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using SHARP2 Antibody (HY-P87071, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for SHARP2 Antibody (YA6764)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of MCF-7 cells labeling SHARP2 with SHARP2 Antibody (HY-P87071) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with SHARP2 Antibody (HY-P87071) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for SHARP2 Antibody (YA6764)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of U2OS cells labeling SHARP2 with SHARP2 Antibody (HY-P87071) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with SHARP2 Antibody (HY-P87071) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for SHARP2 Antibody (YA6764)
  • Experimental Validation Results for SHARP2 Antibody (YA6764)
  • Experimental Validation Results for SHARP2 Antibody (YA6764)
  • Experimental Validation Results for SHARP2 Antibody (YA6764)
  • Experimental Validation Results for SHARP2 Antibody (YA6764)
  • Experimental Validation Results for SHARP2 Antibody (YA6764)
  • Experimental Validation Results for SHARP2 Antibody (YA6764)
  • SHARP2 Antibody (YA6764) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from Hela(lane2(20μg), MDA-MB-231(lane3(20μg), 293T(lane4(20μg) and A431(lane5(20μg) using SHARP2 Antibody (YA6764)(HY-P87071). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • SHARP2 Antibody (YA6764) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue using SHARP2 Antibody (HY-P87071, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • SHARP2 Antibody (YA6764) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using SHARP2 Antibody (HY-P87071, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • SHARP2 Antibody (YA6764) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human esophageal cancer tissue using SHARP2 Antibody (HY-P87071, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • SHARP2 Antibody (YA6764) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using SHARP2 Antibody (HY-P87071, 1/1000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • SHARP2 Antibody (YA6764) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of MCF-7 cells labeling SHARP2 with SHARP2 Antibody (HY-P87071) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with SHARP2 Antibody (HY-P87071) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • SHARP2 Antibody (YA6764) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of U2OS cells labeling SHARP2 with SHARP2 Antibody (HY-P87071) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with SHARP2 Antibody (HY-P87071) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • SHARP2 Antibody (YA6764) Product Image WB
  • SHARP2 Antibody (YA6764) Product Image IHC-P
  • SHARP2 Antibody (YA6764) Product Image IHC-P
  • SHARP2 Antibody (YA6764) Product Image IHC-P
  • SHARP2 Antibody (YA6764) Product Image IHC-P
  • SHARP2 Antibody (YA6764) Product Image ICC
  • SHARP2 Antibody (YA6764) Product Image ICC