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ALDH7A1 Antibody (YA2034)

(Synonyms: aldehyde dehydrogenase 7 family member A1; EPD; PDE; ATQ1)
Customer Review

Based on 1 Customer Validation

ALDH7A1 Antibody (YA2034) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to ALDH7A1.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, ICC/IF, IP

  • Reactivity :

    Human, Mouse

  • Formulation:

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Conjugation:
    Non-conjugated
ALL WB ICC
  • Experimental Validation Results for ALDH7A1 Antibody (YA2034)
  • Experimental Validation Results for ALDH7A1 Antibody (YA2034)
  • Experimental Validation Results for ALDH7A1 Antibody (YA2034)
  • ALDH7A1 Antibody (YA2034) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:500-1:1000 1:50-1:200 1:20

Product Details

Description

ALDH7A1 Antibody (YA2034) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to ALDH7A1.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse
  • Observed Molecular Weight
    Observed band size: 58 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 58 kDa
Species Reactivity Database
Immunogen

A synthetic peptide of human ALDH7A1 aa16-29.

Sensitivity

Endogenous

Purification

Affinity Purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3104401

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for ALDH7A1 Antibody (YA2034)
    Western blot analysis was performed on extracts from HepG2 (lane 1, 15 μg), HT-29 (lane 2, 15 μg), Hela (lane 3, 15 μg), 293T (lane 4, 15 μg), SH-SY5Y (lane 5, 15 μg), and A549 (lane 6, 15 μg) using ALDH7A1 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (GAPDH, HY-P80137, 1:60000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for ALDH7A1 Antibody (YA2034)
    Immunocytochemistry analysis of Hela cells labeling ALDH7A1 with ALDH7A1 Antibody (HY-P82289) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with ALDH7A1 Antibody (HY-P82289) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for ALDH7A1 Antibody (YA2034)
    Immunocytochemistry analysis of HepG2 cells labeling ALDH7A1 with ALDH7A1 Antibody (HY-P82289) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with ALDH7A1 Antibody (HY-P82289) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

  • Function

    ALDH7A1 multifunctional enzyme mediating important protective effects. Metabolizes betaine aldehyde to betaine, an important cellular osmolyte and methyl donor. Protects cells from oxidative stress by metabolizing a number of lipid peroxidation-derived aldehydes. Involved in lysine catabolism

  • Subcellular Localization

    Cytoplasm, cytosol; Nucleus; Mitochondrion

  • Expression


    Tissue_specificity:Abundant in hepatoma cells and fetal cochlea, ovary, eye, heart, adrenal gland, liver and kidney. Low levels present in human peripheral blood leukocytes and fetal brain, thymus, spleen, skeletal muscle, lung and tongue

  • Isoforms & Post-Translational Modification

    P49419 has 3 isomers: P49419-1: 58487 Da (predicted); P49419-2: 55366 Da (predicted); P49419-4: 51411 Da (predicted).

  • Subunit

    Homotetramer

  • SwissProt ID

    P49419

  • Gene ID
    501 [NCBI]
  • Synonyms

    aldehyde dehydrogenase 7 family member A1; EPD; PDE; ATQ1

  • Research Field

    Signal Transduction

ALDH7A1 Antibody (YA2034) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis was performed on extracts from HepG2 (lane 1, 15 μg), HT-29 (lane 2, 15 μg), Hela (lane 3, 15 μg), 293T (lane 4, 15 μg), SH-SY5Y (lane 5, 15 μg), and A549 (lane 6, 15 μg) using ALDH7A1 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (GAPDH, HY-P80137, 1:60000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling ALDH7A1 with ALDH7A1 Antibody (HY-P82289) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with ALDH7A1 Antibody (HY-P82289) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of HepG2 cells labeling ALDH7A1 with ALDH7A1 Antibody (HY-P82289) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with ALDH7A1 Antibody (HY-P82289) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • ALDH7A1 Antibody (YA2034) Product Image
    1/16
  • Experimental Validation Results for ALDH7A1 Antibody (YA2034)
  • Experimental Validation Results for ALDH7A1 Antibody (YA2034)
  • Experimental Validation Results for ALDH7A1 Antibody (YA2034)
  • ALDH7A1 Antibody (YA2034) Product Image
  • Experimental Validation Results for ALDH7A1 Antibody (YA2034)
    1/1
    MedchemExpress Validation
     Western blot analysis was performed on extracts from HepG2 (lane 1, 15 μg), HT-29 (lane 2, 15 μg), Hela (lane 3, 15 μg), 293T (lane 4, 15 μg), SH-SY5Y (lane 5, 15 μg), and A549 (lane 6, 15 μg) using ALDH7A1 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (GAPDH, HY-P80137, 1:60000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for ALDH7A1 Antibody (YA2034)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling ALDH7A1 with ALDH7A1 Antibody (HY-P82289) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with ALDH7A1 Antibody (HY-P82289) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for ALDH7A1 Antibody (YA2034)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of HepG2 cells labeling ALDH7A1 with ALDH7A1 Antibody (HY-P82289) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with ALDH7A1 Antibody (HY-P82289) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for ALDH7A1 Antibody (YA2034)
  • Experimental Validation Results for ALDH7A1 Antibody (YA2034)
  • Experimental Validation Results for ALDH7A1 Antibody (YA2034)
  • ALDH7A1 Antibody (YA2034) Product Image
    MedchemExpress Validation

    Western blot analysis was performed on extracts from HepG2 (lane 1, 15 μg), HT-29 (lane 2, 15 μg), Hela (lane 3, 15 μg), 293T (lane 4, 15 μg), SH-SY5Y (lane 5, 15 μg), and A549 (lane 6, 15 μg) using ALDH7A1 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (GAPDH, HY-P80137, 1:60000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • ALDH7A1 Antibody (YA2034) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of Hela cells labeling ALDH7A1 with ALDH7A1 Antibody (HY-P82289) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with ALDH7A1 Antibody (HY-P82289) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • ALDH7A1 Antibody (YA2034) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of HepG2 cells labeling ALDH7A1 with ALDH7A1 Antibody (HY-P82289) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with ALDH7A1 Antibody (HY-P82289) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • ALDH7A1 Antibody (YA2034) Product Image WB
  • ALDH7A1 Antibody (YA2034) Product Image ICC
  • ALDH7A1 Antibody (YA2034) Product Image ICC