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Junctional Adhesion Molecule 1 Antibody (YA3025)

(Synonyms: F11R; JAM1; JCAM; Junctional adhesion molecule A; JAM-A; Junctional adhesion molecule 1; JAM-1; Platelet F11 receptor; Platelet adhesion molecule 1; PAM-1; CD321)
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Based on 1 Customer Validation

Junctional Adhesion Molecule 1 Antibody (YA3025) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Junctional Adhesion Molecule 1.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, ICC/IF, IP, FC

  • Reactivity :

    Human

  • Formulation:

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for Junctional Adhesion Molecule 1 Antibody (YA3025)
  • Junctional Adhesion Molecule 1 Antibody (YA3025) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
FC Info
FC: Flow Cytometry
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:500-1:1000 1:20-1:50 1:200-1:500 1:20-1:50

Product Details

Description

Junctional Adhesion Molecule 1 Antibody (YA3025) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Junctional Adhesion Molecule 1.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 37 kD Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
Species Reactivity Database

Entrez Gene: 50848 Human

SwissProt: Q9Y624 Human

Immunogen

Recombinant protein (aa28-238) of human Junctional Adhesion Molecule 1/JAM-A.

Sensitivity

Endogenous

Purification

Affinity Purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Junctional Adhesion Molecule 1 Antibody (YA3025)
    Western blot analysis was performed on protein extracts (25 μg) from HeLa (lane 2), A549 (lane 3), Caco-2 (lane 4), and 293T (lane 5) using Junctional Adhesion Molecule 1 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

Background

  • Function

    Junctional Adhesion Molecule 1 is a Seems to play a role in epithelial tight junction formation. Appears early in primordial forms of cell junctions and recruits PARD3. The association of the PARD6-PARD3 complex may prevent the interaction of PARD3 with JAM1, thereby preventing tight junction assembly. Plays a role in regulating monocyte transmigration involved in integrity of epithelial barrier. Ligand for integrin alpha-L/beta-2 involved in memory T-cell and neutrophil transmigration. Involved in platelet activation; (Microbial infection) Acts as a receptor for Mammalian reovirus sigma-1; (Microbial infection) Acts as a receptor for Human Rotavirus strain Wa[1][2][3].

  • Subcellular Localization

    Cell junction, tight junction; Cell membrane; Single-pass type I membrane protein

  • Expression


    Tissue_specificity:It is expressed (at the protein level) in endothelial cells, epithelial cells, and leukocytes.

  • Isoforms & Post-Translational Modification

    Q9Y624 has 2 isomers: Q9Y624-1: 32583 Da (predicted); Q9Y624-2: 27208 Da (predicted).
    N-glycosylated;(Microbial infection) Cleaved by H.pylori virulence factor PqqE. Cleavage leads to altered tight junction functions

  • Subunit

    Interacts with the ninth PDZ domain of MPDZ (PubMed:11489913). Interacts with the first PDZ domain of PARD3 (PubMed:11489913). The association between PARD3 and PARD6B probably disrupts this interaction (By similarity). Interacts with ITGAL (via I-domain) (PubMed:15528364). Interacts with CD151 (PubMed:35067832)

  • SwissProt ID

    Q9Y624

  • Gene ID
    50848 [NCBI]
  • Synonyms

    F11R; JAM1; JCAM; Junctional adhesion molecule A; JAM-A; Junctional adhesion molecule 1; JAM-1; Platelet F11 receptor; Platelet adhesion molecule 1; PAM-1; CD321

  • Research Field

    Cardiovascular

Junctional Adhesion Molecule 1 Antibody (YA3025) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis was performed on protein extracts (25 μg) from HeLa (lane 2), A549 (lane 3), Caco-2 (lane 4), and 293T (lane 5) using Junctional Adhesion Molecule 1 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • Junctional Adhesion Molecule 1 Antibody (YA3025) Product Image
    1/16
  • Experimental Validation Results for Junctional Adhesion Molecule 1 Antibody (YA3025)
  • Junctional Adhesion Molecule 1 Antibody (YA3025) Product Image
  • Experimental Validation Results for Junctional Adhesion Molecule 1 Antibody (YA3025)
    1/1
    MedChemExpress Validation
     Western blot analysis was performed on protein extracts (25 μg) from HeLa (lane 2), A549 (lane 3), Caco-2 (lane 4), and 293T (lane 5) using Junctional Adhesion Molecule 1 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).
  • Experimental Validation Results for Junctional Adhesion Molecule 1 Antibody (YA3025)
  • Junctional Adhesion Molecule 1 Antibody (YA3025) Product Image
    MedChemExpress Validation

    Western blot analysis was performed on protein extracts (25 μg) from HeLa (lane 2), A549 (lane 3), Caco-2 (lane 4), and 293T (lane 5) using Junctional Adhesion Molecule 1 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • Junctional Adhesion Molecule 1 Antibody (YA3025) Product Image WB