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  5. alpha Actinin 4 Antibody (YA2062)(PBS only)

alpha Actinin 4 Antibody (YA2062)(PBS only)

Cat. No.: HY-P82317A
User Guide for Antibodies Technical Support

alpha Actinin 4 Antibody (YA2062) is a rabbit-derived non-conjugated IgG monoclonal antibody (Clone NO.: YA2062), targeting alpha Actinin 4. It can be used as a loading control antibody.

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Tamaño Stock
50 μL   Obtener un presupuesto  
100 μL   Obtener un presupuesto  

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Top Publications Citing Use of Products
  • WB: Western Blot;
  • IHC-P: Immunohistochemistry-Paraffin;
  • IHC-F: Immunohistochemistry-Frozen;
  • ICC/IF: Immunocytochemistry/Immunofluorescence;
  • IF-Tissue: Immunofluorescence-Tissue;
  • mIHC: Multiplex Immunohistochemical;
  • IP: Immunoprecipitation;
  • ChIP: Chromatin Immunoprecipitation;
  • FC: Flow Cytometry;
  • ELISA: Enzyme Linked Immunosorbent Assay

  • Product Detail

  • Background

  • Descripciòn

  • Referencias

Descripciòn

alpha Actinin 4 Antibody (YA2062) is a rabbit-derived non-conjugated IgG monoclonal antibody (Clone NO.: YA2062), targeting alpha Actinin 4. It can be used as a loading control antibody.
Host

Rabbit
Peso molecular
Predicted band size: 105 kDa;
Observed band size: 105 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
Species Reactivity
Human, Mouse, Rat
SwissProt ID
Gene ID
Immunogen

A synthetic peptide of human alpha Actinin 4 aa1-14.
Application &
Dilution Ratio
Application Dilution Ratio
WB
WB: Western Blot
1:500-1:1000
ICC/IF
ICC/IF: Immunocytochemistry/Immunofluorescence
1:50-1:200
IP
IP: Immunoprecipitation
1:20
Pureza Affinity Purified Conjugation Non-conjugated
Isotype IgG  
Appearance

Liquid
Formulation

Supplied in PBS, pH 7.4.
Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
Envío

Shipping with blue ice.
Background
Function：F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein (Probable). Probably involved in vesicular trafficking via its association with the CART complex. The CART complex is necessary for efficient transferrin receptor recycling but not for EGFR degradation (PubMed:15772161). Involved in tight junction assembly in epithelial cells probably through interaction with MICALL2. Links MICALL2 to the actin cytoskeleton and recruits it to the tight junctions (By similarity). May also function as a transcriptional coactivator, stimulating transcription mediated by the nuclear hormone receptors PPARG and RARA (PubMed:22351778). Association with IGSF8 regulates the immune synapse formation and is required for efficient T-cell activation (PubMed:22689882)
Subcellular Localization：Nucleus; Cytoplasm; Cell junction; Cytoplasm, cytoskeleton, stress fiber; Cytoplasm, perinuclear region
Expression：
Tissue_specificity:Widely expressed
Isoforms & Post-Translational Modification：O43707 has 3 isomers: O43707-1: 104854 Da (predicted); O43707-2: 79913 Da (predicted); O43707-3: 59579 Da (predicted).
Subunit：Homodimer; antiparallel. Binds TRIM3 at the N-terminus (PubMed:15772161). Interacts with MICALL2 (preferentially in opened conformation); stimulated by RAB13 activation (By similarity). Identified in a complex with CASK, IQGAP1, MAGI2, NPHS1, SPTAN1 and SPTBN1 (By similarity). Identified in a IGF2BP1-dependent mRNP granule complex containing untranslated mRNAs (PubMed:17289661). Component of the CART complex, at least composed of ACTN4, HGS/HRS, MYO5B and TRIM3 (PubMed:15772161). Interacts with MAGI1 (PubMed:12042308). Interacts with PDLIM2 (By similarity). Interacts with PPARG and RARA (PubMed:22351778). Binds to VCL; this interaction triggers VCL conformational changes (PubMed:15988023). Interacts with SEPTIN14 (PubMed:33228246). Interacts with IGSF8 (PubMed:22689882)
Research Field

Signal Transduction
Synonyms
Alpha-actinin-4 ,Non-muscle alpha-actinin 4 ,ACTN4
Documentación
User Guide for Antibodies (1077 KB)
Referencias

alpha Actinin 4 Antibody (YA2062)(PBS only) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Nombre del producto:
alpha Actinin 4 Antibody (YA2062)(PBS only)
Cat. No.:
HY-P82317A
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