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Giantin Antibody (YA6864)

(Synonyms: 372 kDa Golgi complex-associated protein antibody; GCP antibody; GCP372 antibody; Giantin antibody; GOGB1_HUMAN antibody; GOLGB1 antibody; Golgin B1 antibody; Golgin B1, golgi integral membrane protein antibody; Golgin subfamily B member 1 antibody; GOLIM1 antibody; Macrogolgin antibody; MGC33154 antibody)

Giantin Antibody (YA6864) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Giantin.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, ICC/IF, FC

  • Reactivity :

    Human, Mouse, Rat, Monkey

  • Formulation:

    Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for Giantin Antibody (YA6864)
  • Giantin Antibody (YA6864) Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Data Download
Related Products:
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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
FC Info
FC: Flow Cytometry
Dilution Ratio 1:1000 1:100 1:1000 1:1000

Product Details

Description

Giantin Antibody (YA6864) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Giantin.

  • Host Rabbit
  • Clonality Monoclonal,Recombinant
  • Species Reactivity
    Human, Mouse, Rat, Monkey
  • Observed Molecular Weight
    Observed band size: 376 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 376 kDa
Species Reactivity Database

SwissProt: Q14789 Human

Immunogen

Recombinant protein within human Giantin aa 2,736-3,235 / 3,259.

Purification

affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3719277

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Giantin Antibody (YA6864)
    Western blot analysis of extracts from Hela (lane 1(20μg)) 、A549 (lane 2(20μg)) 、NIH-3T3 (lane 3(20μg)) and PC-12 (lane 4(20μg)) using Giantin Antibody (HY-P87173) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (β Tubulin, 1/5000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

Background

  • Function

    Giantin may participate in forming intercisternal cross-bridges of the Golgi complex

  • Subcellular Localization

    Golgi apparatus membrane

  • Isoforms & Post-Translational Modification

    Q14789 has four isomers: Q14789-1: 376019 Da (predicted); Q14789-2: 377137 Da (predicted); Q14789-3: 372264 Da (predicted); Q14789-4: 367423 Da (predicted).

  • Subunit

    Homodimer; disulfide-linked

  • SwissProt ID

    Q14789

  • Synonyms

    372 kDa Golgi complex-associated protein antibody; GCP antibody; GCP372 antibody; Giantin antibody; GOGB1_HUMAN antibody; GOLGB1 antibody; Golgin B1 antibody; Golgin B1, golgi integral membrane protein antibody; Golgin subfamily B member 1 antibody; GOLIM1 antibody; Macrogolgin antibody; MGC33154 antibody

Giantin Antibody (YA6864) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from Hela (lane 1(20μg)) 、A549 (lane 2(20μg)) 、NIH-3T3 (lane 3(20μg)) and PC-12 (lane 4(20μg)) using Giantin Antibody (HY-P87173) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (β Tubulin, 1/5000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

  • Giantin Antibody (YA6864) Product Image
    1/16
  • Experimental Validation Results for Giantin Antibody (YA6864)
  • Giantin Antibody (YA6864) Product Image
  • Experimental Validation Results for Giantin Antibody (YA6864)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from Hela (lane 1(20μg)) 、A549 (lane 2(20μg)) 、NIH-3T3 (lane 3(20μg)) and PC-12 (lane 4(20μg)) using Giantin Antibody (HY-P87173) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (β Tubulin, 1/5000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Giantin Antibody (YA6864)
  • Giantin Antibody (YA6864) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from Hela (lane 1(20μg)) 、A549 (lane 2(20μg)) 、NIH-3T3 (lane 3(20μg)) and PC-12 (lane 4(20μg)) using Giantin Antibody (HY-P87173) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (β Tubulin, 1/5000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

  • Giantin Antibody (YA6864) Product Image WB