CYP11A1 Antibody (YA7714)

製品番号: HY-P88029: データシート User Guide for Antibodies
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CYP11A1 Antibody (YA7714) is a Mouse-derived and non-conjugated IgG1, Kappaappa monoclonal antibody, targeting to CYP11A1.

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研究用途以外に使用した場合、当社は一切の責任を負いかねます。

容量		在庫状況
50 μL		お問い合わせ
100 μL		お問い合わせ

* アイテムを追加する前、数量をご選択ください

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
説明
参考文献

製品説明

CYP11A1 Antibody (YA7714) is a Mouse-derived and non-conjugated IgG1, Kappaappa monoclonal antibody, targeting to CYP11A1.

Host

Mouse

Clonality

Recombinant

分子量

Predicted band size: 53/57 kDa;

Species Reactivity

Human Predicted Reactivity: Mouse,Rat

Note: The predicted reactivity is for reference only and should not be considered a guarantee of product performance.

SwissProt ID

P05108

Immunogen

KLH conjugated synthetic peptide derived from human CYP11A1

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-2000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:50-200
IHC-F IHC-F: Immunohistochemistry-Frozen	1:50-200
IF-Tissue IF-Tissue: Immunofluorescence-Tissue	1:50-200
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:50-200

Sensitivity	Endogenous	純度	affinity purified by Protein G
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG1,Kappa

Appearance

Liquid

Formulation

Supplied in 10mM TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

輸送条件

Shipping with blue ice.

Background

Function：A cytochrome P450 monooxygenase that catalyzes the side-chain hydroxylation and cleavage of cholesterol to pregnenolone, the precursor of most steroid hormones (PubMed:21636783). Catalyzes three sequential oxidation reactions of cholesterol, namely the hydroxylation at C22 followed with the hydroxylation at C20 to yield 20R,22R-hydroxycholesterol that is further cleaved between C20 and C22 to yield the C21-steroid pregnenolone and 4-methylpentanal (PubMed:21636783). Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate and reducing the second into a water molecule. Two electrons are provided by NADPH via a two-protein mitochondrial transfer system comprising flavoprotein FDXR (adrenodoxin/ferredoxin reductase) and nonheme iron-sulfur protein FDX1 or FDX2 (adrenodoxin/ferredoxin) (PubMed:21636783)

Subcellular Localization：Mitochondrion inner membrane

Expression：Induction: By 8-bromo cyclic AMP

Isoforms & Post-Translational Modification：P05108 has two isomers: P05108-1: 60102 Da (predicted); P05108-2: 42152 Da (predicted).

Subunit：Interacts with FDX1/adrenodoxin

ドキュメンテーション

User Guide for Antibodies (1077 KB)

参考文献

[1]. /biology-dictionary/baf3-cell.html [Content Brief]