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BTF3 Antibody (YA2305)(PBS only)

(Synonyms: NACB; BTF3a; BTF3b; BETA-NAC)

BTF3 Antibody (YA2305) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to BTF3.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, IP

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in PBS, pH 7.4.

  • Conjugation:
    Non-conjugated
ALL WB IHC-P
  • Experimental Validation Results for BTF3 Antibody (YA2305)(PBS only)
  • Experimental Validation Results for BTF3 Antibody (YA2305)(PBS only)
  • Experimental Validation Results for BTF3 Antibody (YA2305)(PBS only)
  • BTF3 Antibody (YA2305)(PBS only) Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:500-1:1000 1:50-1:100 1:20

Product Details

Description

BTF3 Antibody (YA2305) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to BTF3.

  • Host Rabbit
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 22 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 22 kDa
Immunogen

Recombinant protein of human BTF3 aa2-105.

Purification

Affinity Purified

Conjugation

Non-conjugated

Isotype

IgG

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in PBS, pH 7.4.

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for BTF3 Antibody (YA2305)(PBS only)
    Western blot analysis of extracts from C6 (lane 2, 20ug), NIH/3T3 (lane 3, 20ug), A549 (lane 4, 20ug) and RAW264.7 (lane 5, 20ug), Hela (lane 6, 20ug), HT-29 (lane 7, 20ug) using BTF3 Antibody (HY-P82560). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for BTF3 Antibody (YA2305)(PBS only)
    Immunohistochemical analysis of paraffin-embedded Mouse liver tissue using BTF3 Antibody (YA2305). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P82560, 1/100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for BTF3 Antibody (YA2305)(PBS only)
    Immunohistochemical analysis of paraffin-embedded Mouse liver tissue using BTF3 Antibody (YA2305). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P82560, 1/100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Background

  • Function

    BTF3 is a When associated with NACA, prevents inappropriate targeting of non-secretory polypeptides to the endoplasmic reticulum (ER). Binds to nascent polypeptide chains as they emerge from the ribosome and blocks their interaction with the signal recognition particle (SRP), which normally targets nascent secretory peptides to the ER. BTF3 is also a general transcription factor that can form a stable complex with RNA polymerase II. Required for the initiation of transcription

  • Subcellular Localization

    Cytoplasm; Nucleus

  • Isoforms & Post-Translational Modification

    P20290 has 2 isomers: P20290-1: 22168 Da (predicted); P20290-2: 17699 Da (predicted).

  • Subunit

    Part of the nascent polypeptide-associated complex (NAC), which is a heterodimer of NACA and BTF3 (via NAC-A/B domains). NAC associates with ribosomes through the BTF3/NACB subunit. Both subunits can contact nascent polypeptide chains

  • SwissProt ID

    P20290

  • Gene ID
    689 [NCBI]
  • Synonyms

    NACB; BTF3a; BTF3b; BETA-NAC

  • Research Field

    Immunology

BTF3 Antibody (YA2305)(PBS only) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from C6 (lane 2, 20ug), NIH/3T3 (lane 3, 20ug), A549 (lane 4, 20ug) and RAW264.7 (lane 5, 20ug), Hela (lane 6, 20ug), HT-29 (lane 7, 20ug) using BTF3 Antibody (HY-P82560). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded Mouse liver tissue using BTF3 Antibody (YA2305). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P82560, 1/100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded Mouse liver tissue using BTF3 Antibody (YA2305). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P82560, 1/100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • BTF3 Antibody (YA2305)(PBS only) Product Image
    1/16
  • Experimental Validation Results for BTF3 Antibody (YA2305)(PBS only)
  • Experimental Validation Results for BTF3 Antibody (YA2305)(PBS only)
  • Experimental Validation Results for BTF3 Antibody (YA2305)(PBS only)
  • BTF3 Antibody (YA2305)(PBS only) Product Image
  • Experimental Validation Results for BTF3 Antibody (YA2305)(PBS only)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from C6 (lane 2, 20ug), NIH/3T3 (lane 3, 20ug), A549 (lane 4, 20ug) and RAW264.7 (lane 5, 20ug), Hela (lane 6, 20ug), HT-29 (lane 7, 20ug) using BTF3 Antibody (HY-P82560). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for BTF3 Antibody (YA2305)(PBS only)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded Mouse liver tissue using BTF3 Antibody (YA2305). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P82560, 1/100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for BTF3 Antibody (YA2305)(PBS only)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded Mouse liver tissue using BTF3 Antibody (YA2305). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P82560, 1/100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for BTF3 Antibody (YA2305)(PBS only)
  • Experimental Validation Results for BTF3 Antibody (YA2305)(PBS only)
  • Experimental Validation Results for BTF3 Antibody (YA2305)(PBS only)
  • BTF3 Antibody (YA2305)(PBS only) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from C6 (lane 2, 20ug), NIH/3T3 (lane 3, 20ug), A549 (lane 4, 20ug) and RAW264.7 (lane 5, 20ug), Hela (lane 6, 20ug), HT-29 (lane 7, 20ug) using BTF3 Antibody (HY-P82560). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • BTF3 Antibody (YA2305)(PBS only) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded Mouse liver tissue using BTF3 Antibody (YA2305). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P82560, 1/100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • BTF3 Antibody (YA2305)(PBS only) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded Mouse liver tissue using BTF3 Antibody (YA2305). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P82560, 1/100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • BTF3 Antibody (YA2305)(PBS only) Product Image WB
  • BTF3 Antibody (YA2305)(PBS only) Product Image IHC-P
  • BTF3 Antibody (YA2305)(PBS only) Product Image IHC-P