BTF3 Antibody (YA2305)(PBS only)

Cat. No.: HY-P82560A: Data Sheet User Guide for Antibodies
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BTF3 Antibody (YA2305) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to BTF3.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

BTF3 Antibody (YA2305) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to BTF3.

Host

Rabbit

Molecular Weight

Predicted band size: 22 kDa;

Observed band size: 22 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

P20290

Gene ID

689 [NCBI]

Immunogen

Recombinant protein of human BTF3 aa2-105.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:50-1:100
IP IP: Immunoprecipitation	1:20

Purity	Affinity Purified	Conjugation	Non-conjugated
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：When associated with NACA, prevents inappropriate targeting of non-secretory polypeptides to the endoplasmic reticulum (ER). Binds to nascent polypeptide chains as they emerge from the ribosome and blocks their interaction with the signal recognition particle (SRP), which normally targets nascent secretory peptides to the ER. BTF3 is also a general transcription factor that can form a stable complex with RNA polymerase II. Required for the initiation of transcription

Subcellular Localization：Cytoplasm; Nucleus

Isoforms & Post-Translational Modification：P20290 has 2 isomers: P20290-1: 22168 Da (predicted); P20290-2: 17699 Da (predicted).

Subunit：Part of the nascent polypeptide-associated complex (NAC), which is a heterodimer of NACA and BTF3 (via NAC-A/B domains). NAC associates with ribosomes through the BTF3/NACB subunit. Both subunits can contact nascent polypeptide chains

Research Field

Immunology

Synonyms

NACB; BTF3a; BTF3b; BETA-NAC

Documentation

User Guide for Antibodies (1077 KB)