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Casein Kinase 2 beta Antibody (YA563)

(Synonyms: CSNK2B; CK2N; G5A; Casein kinase II subunit beta; CK II beta; Phosvitin; Protein G5a)
Customer Review

Based on 1 Customer Validation

Casein Kinase 2 beta Antibody (YA563) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Casein Kinase 2 beta.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-F, IHC-P, ICC/IF

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol and 0.05% BSA. Preservative: 0.01% Sodium azide

  • Conjugation:
    Non-conjugated
ALL WB ICC
  • Experimental Validation Results for Casein Kinase 2 beta Antibody (YA563)
  • Experimental Validation Results for Casein Kinase 2 beta Antibody (YA563)
  • Experimental Validation Results for Casein Kinase 2 beta Antibody (YA563)
  • Experimental Validation Results for Casein Kinase 2 beta Antibody (YA563)
  • Casein Kinase 2 beta Antibody (YA563) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
IHC-F Info
IHC-F: Immunohistochemistry-Frozen
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
Dilution Ratio 1:500-1:1000 1:50-1:100 1:50-1:100 1:50-1:200

Product Details

Description

Casein Kinase 2 beta Antibody (YA563) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Casein Kinase 2 beta.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 25 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 25 kDa
Species Reactivity Database
Immunogen

Synthetic peptide corresponding to Human Casein Kinase 2 beta.The exact sequence is proprietary to MCE.

Sensitivity

Endogenous

Purification

affinity purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3102137

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol and 0.05% BSA. Preservative: 0.01% Sodium azide

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Casein Kinase 2 beta Antibody (YA563)
    Western blot analysis of extracts from M-spleen (lane 1) and K562 (lane 2) and M-liver (lane 3) using Casein Kinase 2 beta antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/1000) and loading control antibody (GAPDH, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.
  • Experimental Validation Results for Casein Kinase 2 beta Antibody (YA563)
    Western blot analysis was performed on extracts from Ramos (lane 1, 15 μg), U251MG (lane 2, 15 μg), C2C12 (lane 3, 15 μg), and H9c2 (lane 4, 15 μg) using Casein Kinase 2 beta Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P80438, 1:5000 dilution) was incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:40000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for Casein Kinase 2 beta Antibody (YA563)
    Immunocytochemistry analysis of Hela cells labeling Casein Kinase 2 beta with Casein Kinase 2 beta Antibody (HY-P80586) at 1/100 dilution . Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with Casein Kinase 2 beta Antibody (HY-P80586) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green） was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Casein Kinase 2 beta Antibody (YA563)
    Immunocytochemistry analysis of HePG2 cells labeling Casein Kinase 2 beta with Casein Kinase 2 beta Antibody (HY-P80586) at 1/100 dilution . Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with Casein Kinase 2 beta Antibody (HY-P80586) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green） was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

  • Function

    Casein Kinase 2 beta is a Regulatory subunit of casein kinase II/CK2. As part of the kinase complex regulates the basal catalytic activity of the alpha subunit a constitutively active serine/threonine-protein kinase that phosphorylates a large number of substrates containing acidic residues C-terminal to the phosphorylated serine or threonine. Participates in Wnt signaling; (Microbial infection) Upon infection with Epstein-Barr virus (EBV), the interaction with viral EBNA1 increases the association of CK2 with PML proteins, which increases PML phosphorylation by CK2, triggering the polyubiquitylation and degradation of PML. Seems to also suppress EBV reactivation by mediating ARK2N and JUN at the Z promoter which inhibits BZLF1 transcrition[1][2][3][4][5].

  • Subcellular Localization

    Nucleus

  • Subunit

    Casein kinase II/CK2 is a tetramer composed of an alpha subunit, an alpha' subunit and two beta subunits. The beta subunit dimerization is mediated by zinc ions. Interacts with DYNLT2 (By similarity). Interacts with CD163. Also a component of a CK2-SPT16-SSRP1 complex composed of SSRP1, SUPT16H, CSNK2A1, CSNK2A2 and CSNK2B, the complex associating following UV irradiation. Interacts with MUSK; mediates phosphorylation of MUSK by CK2. Interacts with FGF1; this interaction is increased in the presence of FIBP, suggesting a possible cooperative interaction between CSNKB and FIBP in binding to FGF1. Interacts (via KSSR motif) with ARK2N (PubMed:24216761). Interacts with JUN and ARK2N; mediates the interaction between ARK2N and JUN (PubMed:31341047)

  • SwissProt ID

    P67870

  • Gene ID
    1460 [NCBI]
  • Synonyms

    CSNK2B; CK2N; G5A; Casein kinase II subunit beta; CK II beta; Phosvitin; Protein G5a

  • Research Field

    Cell Biology

[1]. Keller DM, et al. A DNA damage-induced p53 serine 392 kinase complex contains CK2, hSpt16, and SSRP1. Mol Cell. 2001 Feb;7(2):283-92. [Content Brief]

[2]. Cheusova T, et al. Casein kinase 2-dependent serine phosphorylation of MuSK regulates acetylcholine receptor aggregation at the neuromuscular junction. Genes Dev. 2006 Jul 1;20(13):1800-16. [Content Brief]

[3]. Sivachandran N, et al. Epstein-Barr virus nuclear antigen 1 Hijacks the host kinase CK2 to disrupt PML nuclear bodies. J Virol. 2010 Nov;84(21):11113-23. [Content Brief]

[4]. Cao JY, et al. Identification of a novel protein interaction motif in the regulatory subunit of casein kinase 2. Mol Cell Biol. 2014 Jan;34(2):246-58. [Content Brief]

[5]. Siddiqi UZ, et al. Identification of ARKL1 as a Negative Regulator of Epstein-Barr Virus Reactivation. J Virol. 2019 Oct 15;93(20):. [Content Brief]

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Casein Kinase 2 beta Antibody (YA563) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis of extracts from M-spleen (lane 1) and K562 (lane 2) and M-liver (lane 3) using Casein Kinase 2 beta antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/1000) and loading control antibody (GAPDH, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.

  • 1/16

    MedchemExpress Validation
     Western blot analysis was performed on extracts from Ramos (lane 1, 15 μg), U251MG (lane 2, 15 μg), C2C12 (lane 3, 15 μg), and H9c2 (lane 4, 15 μg) using Casein Kinase 2 beta Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P80438, 1:5000 dilution) was incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:40000 dilution) was then applied for 40 minutes at 37°C.

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling Casein Kinase 2 beta with Casein Kinase 2 beta Antibody (HY-P80586) at 1/100 dilution . Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with Casein Kinase 2 beta Antibody (HY-P80586) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green） was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of HePG2 cells labeling Casein Kinase 2 beta with Casein Kinase 2 beta Antibody (HY-P80586) at 1/100 dilution . Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with Casein Kinase 2 beta Antibody (HY-P80586) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green） was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Casein Kinase 2 beta Antibody (YA563) Product Image
    1/16
  • Experimental Validation Results for Casein Kinase 2 beta Antibody (YA563)
  • Experimental Validation Results for Casein Kinase 2 beta Antibody (YA563)
  • Experimental Validation Results for Casein Kinase 2 beta Antibody (YA563)
  • Experimental Validation Results for Casein Kinase 2 beta Antibody (YA563)
  • Casein Kinase 2 beta Antibody (YA563) Product Image
  • Experimental Validation Results for Casein Kinase 2 beta Antibody (YA563)
    1/1
    MedchemExpress Validation
     Western blot analysis of extracts from M-spleen (lane 1) and K562 (lane 2) and M-liver (lane 3) using Casein Kinase 2 beta antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/1000) and loading control antibody (GAPDH, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.
  • Experimental Validation Results for Casein Kinase 2 beta Antibody (YA563)
    1/1
    MedchemExpress Validation
     Western blot analysis was performed on extracts from Ramos (lane 1, 15 μg), U251MG (lane 2, 15 μg), C2C12 (lane 3, 15 μg), and H9c2 (lane 4, 15 μg) using Casein Kinase 2 beta Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P80438, 1:5000 dilution) was incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:40000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for Casein Kinase 2 beta Antibody (YA563)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling Casein Kinase 2 beta with Casein Kinase 2 beta Antibody (HY-P80586) at 1/100 dilution . Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with Casein Kinase 2 beta Antibody (HY-P80586) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green） was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Casein Kinase 2 beta Antibody (YA563)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of HePG2 cells labeling Casein Kinase 2 beta with Casein Kinase 2 beta Antibody (HY-P80586) at 1/100 dilution . Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with Casein Kinase 2 beta Antibody (HY-P80586) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green） was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Casein Kinase 2 beta Antibody (YA563)
  • Experimental Validation Results for Casein Kinase 2 beta Antibody (YA563)
  • Experimental Validation Results for Casein Kinase 2 beta Antibody (YA563)
  • Experimental Validation Results for Casein Kinase 2 beta Antibody (YA563)
  • Casein Kinase 2 beta Antibody (YA563) Product Image
    MedchemExpress Validation

    Western blot analysis of extracts from M-spleen (lane 1) and K562 (lane 2) and M-liver (lane 3) using Casein Kinase 2 beta antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/1000) and loading control antibody (GAPDH, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.

  • Casein Kinase 2 beta Antibody (YA563) Product Image
    MedchemExpress Validation

    Western blot analysis was performed on extracts from Ramos (lane 1, 15 μg), U251MG (lane 2, 15 μg), C2C12 (lane 3, 15 μg), and H9c2 (lane 4, 15 μg) using Casein Kinase 2 beta Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P80438, 1:5000 dilution) was incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:40000 dilution) was then applied for 40 minutes at 37°C.

  • Casein Kinase 2 beta Antibody (YA563) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of Hela cells labeling Casein Kinase 2 beta with Casein Kinase 2 beta Antibody (HY-P80586) at 1/100 dilution . Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with Casein Kinase 2 beta Antibody (HY-P80586) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green） was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Casein Kinase 2 beta Antibody (YA563) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of HePG2 cells labeling Casein Kinase 2 beta with Casein Kinase 2 beta Antibody (HY-P80586) at 1/100 dilution . Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with Casein Kinase 2 beta Antibody (HY-P80586) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green） was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Casein Kinase 2 beta Antibody (YA563) Product Image WB
  • Casein Kinase 2 beta Antibody (YA563) Product Image WB
  • Casein Kinase 2 beta Antibody (YA563) Product Image ICC
  • Casein Kinase 2 beta Antibody (YA563) Product Image ICC