Caspase-1 Antibody (YA5936)
(Synonyms: IL1BC, IL1BCE, CASP1, Caspase-1, CASP-1, Interleukin-1 beta convertase, Interleukin-1 beta-converting enzyme, p45, IL-1BC, ICE, IL-1 beta-converting enzyme)
Based on 1 Customer Validation
Caspase-1 Antibody (YA5936) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Caspase-1.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, IHC-P, ICC/IF, IP, ELISA
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Reactivity :
Human, Mouse, Rat
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Formulation:
Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
IHC-P
IHC-P: Immunohistochemistry-Paraffin
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WB
WB: Western Blot
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ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
ELISA
ELISA: Enzyme Linked Immunosorbent Assay
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IP
IP: Immunoprecipitation
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|---|---|---|---|---|---|
| Dilution Ratio | 1:500-1:1000 | 1:2000-1:10000 | 1:200-1:1000 | 1:5000-1:20000 | 1:50-1:200 |
Product Details
Caspase-1 Antibody (YA5936) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Caspase-1.
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Host Rabbit
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Clonality Monoclonal
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Species ReactivityHuman, Mouse, Rat
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Observed Molecular WeightObserved band size: 45 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 45 kDa
Protein A
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
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Concentration
Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
Caspase-1 is the effector protease of canonical inflammasomes, where inflammasome activation enables processing and secretion of IL-1β and IL-18[1]. Mechanistically, caspase-1/11 cleaves gasdermin D (GSDMD), and GSDMD functions downstream as a pyroptosis executioner while also providing negative feedback on caspase-1/11 activation[2]. In endothelial models, homocysteine and lipopolysaccharide induced NLRP3 inflammasome assembly, caspase-1 activation, IL-1β cleavage, and caspase-1-dependent pyroptosis linked to endothelial dysfunction[3]. In respiratory infection models, Nontypeable Haemophilus influenzae activated caspase-1 and caspase-1 inhibition significantly reduced IL-1β release in respiratory cells and human lung tissue[4]. Compared with related isoforms, caspase-1 primarily controlled infected macrophage pyroptosis and IL-18 production in pneumonic melioidosis, whereas caspase-11 mediated infected lung epithelial-cell pyroptosis[5]. More broadly, murine caspase-11 and human caspase-4/5 define the noncanonical inflammasome pathway as cytosolic LPS sensors, separating their upstream recognition role from canonical procaspase-1 inflammasome activation[6]. For experimental applications, caspase-1 inhibitors can test IL-1β-dependent inflammatory output, while GSDMD-derived peptide inhibition provides a tool to suppress caspase-1/11 activation and downstream substrate cleavage[2][4].
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Subcellular Localization
Cytoplasm; Cell membrane
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Expression
Tissue_specificity:It is expressed at high levels in the spleen and lungs. It can also be detected in the liver, heart, small intestine, colon, thymus, prostate, skeletal muscle, peripheral blood leukocytes, kidneys, and testes. Expression was not detected in brain tissue.
Induction:Transcription and translation induced by M.tuberculosis and a number of different M.tuberculosis components; EsxA is the most potent activator tested (at protein level) (PubMed:20148899) -
Isoforms & Post-Translational Modification
P29466 has 5 isomers: P29466-1: 45159 Da (predicted); P29466-2: 42888 Da (predicted); P29466-3: 35019 Da (predicted); P29466-4: 29821 Da (predicted); P29466-5: 10417 Da (predicted).
The two subunits are derived from the precursor sequence by an autocatalytic mechanism;Ubiquitinated via 'Lys-11'-linked polyubiquitination. Deubiquitinated by USP8;Cleavage in the interdomain linker region is required to induce pyroptosis -
Subunit
Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 20 kDa (Caspase-1 subunit p20) and a 10 kDa (Caspase-1 subunit p10) subunit (PubMed:32109412, PubMed:32553275, PubMed:8044845, PubMed:9987822). May be a component of the inflammasome, a protein complex which also includes PYCARD, CARD8 and NLRP2 and whose function would be the activation of pro-inflammatory caspases (PubMed:15030775, PubMed:33420033). Component of the AIM2 PANoptosome complex, a multiprotein complex that drives inflammatory cell death (PANoptosis) (By similarity). Interacts with CARD8; interacts with the released C-terminus of CARD8 which forms an inflammasome and directly activates CASP1 to promote pyroptosis (PubMed:32051255). Both the p10 and p20 subunits interact with MEFV (PubMed:16785446). Interacts with CARD17P/INCA and CARD18 (PubMed:11051551, PubMed:15383541). Interacts with SERPINB1; this interaction regulates CASP1 activity (PubMed:30692621)
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SwissProt ID
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Synonyms
IL1BC, IL1BCE, CASP1, Caspase-1, CASP-1, Interleukin-1 beta convertase, Interleukin-1 beta-converting enzyme, p45, IL-1BC, ICE, IL-1 beta-converting enzyme
Documentation
References
[1]. Dinarello CA. Mutations in cryopyrin: bypassing roadblocks in the caspase 1 inflammasome for interleukin-1beta secretion and disease activity. Arthritis Rheum. 2007 Sep;56(9):2817-22. doi: 10.1002/art.22841. PMID: 17763412. et al. Mutations in cryopyrin: bypassing roadblocks in the caspase 1 inflammasome for interleukin-1beta secretion and disease activity. Arthritis Rheum. 2007 Sep;56(9):2817-22. [Content Brief]
[2]. Hu Y, et al. The Gasdermin D N-terminal fragment acts as a negative feedback system to inhibit inflammasome-mediated activation of Caspase-1/11. Proc Natl Acad Sci U S A. 2022 Nov 8;119(45):e2210809119. [Content Brief]
[3]. Xi H, et al. Caspase-1 Inflammasome Activation Mediates Homocysteine-Induced Pyrop-Apoptosis in Endothelial Cells. Circ Res. 2016 May 13;118(10):1525-39. [Content Brief]
[5]. Wang J, et al. Caspase-11-dependent pyroptosis of lung epithelial cells protects from melioidosis while caspase-1 mediates macrophage pyroptosis and production of IL-18. PLoS Pathog. 2018 May 23;14(5):e1007105. [Content Brief]
[6]. Wu J, et al. Pyroptosis by caspase-11 inflammasome-Gasdermin D pathway in autoimmune diseases. Pharmacol Res. 2021 Mar;165:105408. [Content Brief]
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