MRCK alpha Antibody
(Synonyms: KIAA0451, CDC42BPA, Serine/threonine-protein kinase MRCK alpha, CDC42-binding protein kinase alpha, DMPK-like alpha, Myotonic dystrophy kinase-related CDC42-binding kinase alpha, MRCK alpha, Myotonic dystrophy protein kinase-like alpha)
Based on 1 Customer Validation
MRCK alpha Antibody is a Rabbit-derived and non-conjugated IgG Polyclonal antibody, targeting to MRCK alpha.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, ICC/IF
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Reactivity :
Human, Mouse, Rat
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Formulation:
Supplied in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
|---|---|---|
| Dilution Ratio | 1:500-2000 | 1:50-200 |
Product Details
MRCK alpha Antibody is a Rabbit-derived and non-conjugated IgG Polyclonal antibody, targeting to MRCK alpha.
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Host Rabbit
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Clonality Polyclonal
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Species ReactivityHuman, Mouse, Rat
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Observed Molecular WeightObserved band size: 186 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 197 kDa
Synthetic peptide corresponding to the center region of human MRCK alpha(aa636-673).
Endogenous
affinity purified.
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
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Concentration
Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
MRCK alpha is a Serine/threonine-protein kinase which is an important downstream effector of CDC42 and plays a role in the regulation of cytoskeleton reorganization and cell migration. Regulates actin cytoskeletal reorganization via phosphorylation of PPP1R12C and MYL9/MLC2. In concert with MYO18A and LURAP1, is involved in modulating lamellar actomyosin retrograde flow that is crucial to cell protrusion and migration. Phosphorylates: PPP1R12A, LIMK1 and LIMK2. May play a role in TFRC-mediated iron uptake. In concert with FAM89B/LRAP25 mediates the targeting of LIMK1 to the lamellipodium resulting in its activation and subsequent phosphorylation of CFL1 which is important for lamellipodial F-actin regulation (By similarity). Triggers the formation of an extrusion apical actin ring required for epithelial extrusion of apoptotic cells[1][2][3][4][5][6][7][8][9].
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Subcellular Localization
Cytoplasm; Cell projection, lamellipodium
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Expression
Tissue_Specificity: Abundant in the heart, brain, skeletal muscle, kidney, and pancreas, with little or no expression in the lung and liver.
Induction: Regulated by cellular iron levels. -
Isoforms & Post-Translational Modification
MRCK alpha has 6 isoforms, Q5VT25-1: amino acid length is 1732, molecular weight is 197307 Da (predicted); Q5VT25-2: amino acid length is 1754, molecular weight is 199811 Da (predicted); Q5VT25-3: amino acid length is 1638, molecular weight is 186113 Da (predicted); Q5VT25-4: amino acid length is 1691, molecular weight is 193031 Da (predicted); Q5VT25-5: amino acid length is 1719, molecular weight is 195922 Da (predicted); Q5VT25-6: amino acid length is 1781, molecular weight is 202812 Da (predicted).
Proteolytically cleaved by caspases upon apoptosis induction. The cleavage at Asp-478 by CASP3 increases its kinase activity (in vitro). -
Subunit
Homodimer and homotetramer via the coiled coil regions.
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SwissProt ID
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Synonyms
KIAA0451, CDC42BPA, Serine/threonine-protein kinase MRCK alpha, CDC42-binding protein kinase alpha, DMPK-like alpha, Myotonic dystrophy kinase-related CDC42-binding kinase alpha, MRCK alpha, Myotonic dystrophy protein kinase-like alpha
Documentation
[1]. Wilkinson S, et al. Cdc42-MRCK and Rho-ROCK signalling cooperate in myosin phosphorylation and cell invasion. Nat Cell Biol. 2005 Mar;7(3):255-61. [Content Brief]
[2]. Zhao Y, et al. Cloning and chromosomal location of a novel member of the myotonic dystrophy family of protein kinases. J Biol Chem. 1997 Apr 11;272(15):10013-20. [Content Brief]
[3]. Leung T, et al. Myotonic dystrophy kinase-related Cdc42-binding kinase acts as a Cdc42 effector in promoting cytoskeletal reorganization. Mol Cell Biol. 1998 Jan;18(1):130-40. [Content Brief]
[4]. Tan I, et al. Chelerythrine perturbs lamellar actomyosin filaments by selective inhibition of myotonic dystrophy kinase-related Cdc42-binding kinase. FEBS Lett. 2011 May 6;585(9):1260-8. [Content Brief]
[5]. Tan I, et al. A tripartite complex containing MRCK modulates lamellar actomyosin retrograde flow. Cell. 2008 Oct 3;135(1):123-36. [Content Brief]
[6]. Sumi T, et al. Activation of LIM kinases by myotonic dystrophy kinase-related Cdc42-binding kinase alpha. J Biol Chem. 2001 Jun 22;276(25):23092-6. [Content Brief]
[7]. Tan I, et al. Phosphorylation of a novel myosin binding subunit of protein phosphatase 1 reveals a conserved mechanism in the regulation of actin cytoskeleton. J Biol Chem. 2001 Jun 15;276(24):21209-16. [Content Brief]
[8]. Cmejla R, et al. Human MRCKalpha is regulated by cellular iron levels and interferes with transferrin iron uptake. Biochem Biophys Res Commun. 2010 Apr 30;395(2):163-7. [Content Brief]
[9]. Gagliardi PA, et al. MRCKα is activated by caspase cleavage to assemble an apical actin ring for epithelial cell extrusion. J Cell Biol. 2018 Jan 2;217(1):231-249. [Content Brief]
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