CaMKII Antibody (YA4627)

Cat. No.: HY-P84930: Data Sheet COA
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CaMKII Antibody (YA4627) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to CaMKII.

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사이즈	재고	수량
10 μL	해외재고보유	Estimated Time of Arrival: December 31
50 μL	해외재고보유	Estimated Time of Arrival: December 31
100 μL	해외재고보유	Estimated Time of Arrival: December 31
250 μL	견적 받기

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1 Publications Citing Use of MCE CaMKII Antibody (YA4627)

•ACS Nano. 2025 Nov 4;19(43):37617-37632. [Abstract]

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Verification Image
Background
제품 설명

제품 설명

CaMKII Antibody (YA4627) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to CaMKII.

Host

Rabbit

Clonality

Monoclonal,Recombinant

분자량

Predicted band size: 54 kDa;

Observed band size: 50 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat, Monkey, Pig Predicted Reactivity: Pig

Note: The predicted reactivity is for reference only and should not be considered a guarantee of product performance.

SwissProt ID

Q13554 / Q13555 / Q13557 / Q9UQM7

Gene ID

815 [NCBI] / 816 [NCBI] / 817 [NCBI] / 818 [NCBI]

Immunogen

Synthetic peptide within Human CaMKⅡ aa 201-250 / 478.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:2000
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:100
IF-Tissue IF-Tissue: Immunofluorescence-Tissue	1:50-1:200
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:50-1:200
IHC-F IHC-F: Immunohistochemistry-Frozen	1:100
FC FC: Flow Cytometry	1:1000

Purity	Protein A affinity purified.	Conjugation	Non-conjugated
Modification	Unmodified	Isotype	IgG

Appearance

Liquid

Formulation

Supplied in 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

선적

Shipping with blue ice.

Verification Image

ALL WB IHC-P

Western blot analysis of extracts from C6 (lane 2(20μg) and C6 (lane 3(40μg), NIH3T3 (lane 4(20μg) and NIH3T3 (lane 5(40μg) using CaMKII (HY-P84930) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
Immunohistochemical analysis of paraffin-embedded mouse brain tissue using CaMKII Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunohistochemical analysis of paraffin-embedded mouse brain tissue using CaMKII Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Background

Function：Q13554: Calcium/calmodulin-dependent protein kinase that functions autonomously after Ca (2+) /calmodulin-binding and autophosphorylation, and is involved in dendritic spine and synapse formation, neuronal plasticity and regulation of sarcoplasmic reticulum Ca (2+) transport in skeletal muscle (PubMed:16690701). In neurons, plays an essential structural role in the reorganization of the actin cytoskeleton during plasticity by binding and bundling actin filaments in a kinase-independent manner. This structural function is required for correct targeting of CaMK2A, which acts downstream of NMDAR to promote dendritic spine and synapse formation and maintain synaptic plasticity which enables long-term potentiation (LTP) and hippocampus-dependent learning. In developing hippocampal neurons, promotes arborization of the dendritic tree and in mature neurons, promotes dendritic remodeling. Also regulates the migration of developing neurons (PubMed:29100089). Participates in the modulation of skeletal muscle function in response to exercise (PubMed:16690701).
Q13555: Calcium/calmodulin-dependent protein kinase that functions autonomously after Ca (2+) /calmodulin-binding and autophosphorylation, and is involved in sarcoplasmic reticulum Ca (2+) transport in skeletal muscle and may function in dendritic spine and synapse formation and neuronal plasticity (PubMed:16690701). In slow-twitch muscles, is involved in regulation of sarcoplasmic reticulum (SR) Ca (2+) transport and in fast-twitch muscle participates in the control of Ca (2+) release from the SR through phosphorylation of the ryanodine receptor-coupling factor triadin (PubMed:16690701). In the central nervous system, it is involved in the regulation of neurite formation and arborization (PubMed:30184290).
Q13557: Calcium/calmodulin-dependent protein kinase involved in the regulation of Ca (2+) homeostatis and excitation-contraction coupling (ECC) in heart by targeting ion channels, transporters and accessory proteins involved in Ca (2+) influx into the myocyte, Ca (2+) release from the sarcoplasmic reticulum (SR) , SR Ca (2+) uptake and Na (+) and K (+) channel transport. Targets also transcription factors and signaling molecules to regulate heart function.)
Q9UQM7: Calcium/calmodulin-dependent protein kinase that functions autonomously after Ca (2+) /calmodulin-binding and autophosphorylation, and is involved in various processes, such as synaptic plasticity, neurotransmitter release and long-term potentiation (PubMed:14722083). Regulates dendritic spine development (PubMed:28130356). Also regulates the migration of developing neurons (PubMed:29100089). Phosphorylates the transcription factor FOXO3 to activate its transcriptional activity (PubMed:23805378). In response to interferon-gamma (IFN-gamma) stimulation, catalyzes phosphorylation of STAT1, stimulating the JAK-STAT signaling pathway (PubMed:11972023). In response to interferon-beta (IFN-beta) stimulation, stimulates the JAK-STAT signaling pathway (PubMed:35568036).

Subcellular Localization：Q13554: Cytoplasm, cytoskeleton; Cytoplasm, cytoskeleton, microtubule organizing center, centrosome; Sarcoplasmic reticulum membrane; Peripheral membrane protein; Cytoplasmic side; Synapse
Q13555: Sarcoplasmic reticulum membrane; Peripheral membrane protein; Cytoplasmic side
Q13557: Cell membrane, sarcolemma; Peripheral membrane protein; Cytoplasmic side; Sarcoplasmic reticulum membrane; Peripheral membrane protein; Cytoplasmic side
Q9UQM7: Synapse; Postsynaptic density; Cell projection, dendritic spine; Cell projection, dendrite

Expression：
Tissue_specificity: Q13554: Widely expressed. Expressed in adult and fetal brain. Expression is slightly lower in fetal brain. Expressed in skeletal muscle
Q13555: Expressed in skeletal muscle
Q13557: Expressed in cardiac muscle and skeletal muscle. Isoform Delta 3, isoform Delta 2, isoform Delta 8 and isoform Delta 9 are expressed in cardiac muscle. Isoform Delta 11 is expressed in skeletal muscle
Induction: Q13554: Activity is induced in skeletal muscle during exercise
Q13555: Activity is induced in skeletal muscle during exercise
Q13557: Activity is induced in skeletal muscle during exercise

Subunit：Q13554: CAMK2 is composed of 4 different chains: alpha (CAMK2A) , beta (CAMK2B) , gamma (CAMK2G) , and delta (CAMK2D). The different isoforms assemble into homo- or heteromultimeric holoenzymes composed of 12 subunits with two hexameric rings stacked one on top of the other (PubMed:14722083).
Q13555: CAMK2 is composed of 4 different chains: alpha (CAMK2A) , beta (CAMK2B) , gamma (CAMK2G) , and delta (CAMK2D) .
Q13557: CAMK2 is composed of 4 different chains: alpha (CAMK2A) , beta (CAMK2B) , gamma (CAMK2G) , and delta (CAMK2D) .
Q9UQM7: There are 4 genes encoding calcium/calmodulin-dependent protein kinase type II chains: CAMK2A, CAMK2B, CAMK2G and CAMK2D. The corresponding proteins assemble into homo- or heteromultimeric holoenzymes composed of 12 subunits with two hexameric rings stacked one on top of the other (PubMed:14722083, PubMed:29784083).

RRID

AB_3719005

Database

Entrez Gene: 815 Human ; 816 Human ; 817 Human ; 818 Human ; 108058 Mouse ; 12322 Mouse ; 12323 Mouse ; 12325 Mouse ; 171140 Rat ; 24245 Rat ; 24246 Rat ; 25400 Rat

SwissProt: Q13554 Human ; Q13555 Human ; Q13557 Human ; Q9UQM7 Human ; P11798 Mouse ; P28652 Mouse ; Q6PHZ2 Mouse ; Q923T9 Mouse ; P08413 Rat ; P11275 Rat ; P11730 Rat ; P15791 Rat

OMIM: 114078 Human

Synonyms

Calcium/calmodulin dependent protein kinase II alpha antibody; Calcium/calmodulin dependent protein kinase II beta antibody; Calcium/calmodulin dependent protein kinase II delta antibody; Calcium/calmodulin dependent protein kinase II gamma antibody; Calcium/calmodulin-dependent protein kinase type II subunit alpha antibody; CaM kinase II alpha antibody; CaM kinase II antibody; CaM kinase II beta antibody; CaM kinase II delta antibody; CaM kinase II gamma antibody; CaM kinase II subunit alpha antibody; CaMK-II subunit alpha antibody; CAMK2 antibody; Camk2a antibody; CAMK2B antibody; CAMK2D antibody; CAMK2G antibody; CAMKA antibody; KCC2A_HUMAN antibody; CaMKⅡ

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Data Sheet (236 KB) SDS (314 KB)

COA (206 KB)

User Guide for Antibodies (1077 KB)