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CaMKII Antibody (YA4627)

(Synonyms: Calcium/calmodulin dependent protein kinase II alpha antibody; Calcium/calmodulin dependent protein kinase II beta antibody; Calcium/calmodulin dependent protein kinase II delta antibody; Calcium/calmodulin dependent protein kinase II gamma antibody; Calcium/calmodulin-dependent protein kinase type II subunit alpha antibody; CaM kinase II alpha antibody; CaM kinase II antibody; CaM kinase II beta antibody; CaM kinase II delta antibody; CaM kinase II gamma antibody; CaM kinase II subunit alpha antibody; CaMK-II subunit alpha antibody; CAMK2 antibody; Camk2a antibody; CAMK2B antibody; CAMK2D antibody; CAMK2G antibody; CAMKA antibody; KCC2A_HUMAN antibody; CaMKⅡ)
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Based on 1 publication(s) in Google Scholar

CaMKII Antibody (YA4627) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to CaMKII.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, IHC-F, ICC/IF, FC, IF-Tissue

  • Reactivity :

    Human, Mouse, Rat, Monkey, Pig

  • Formulation:

    Supplied in 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Conjugation:
    Non-conjugated
ALL WB IHC-P FC
  • Experimental Validation Results for CaMKII Antibody (YA4627)
  • Experimental Validation Results for CaMKII Antibody (YA4627)
  • Experimental Validation Results for CaMKII Antibody (YA4627)
  • Experimental Validation Results for CaMKII Antibody (YA4627)
  • CaMKII Antibody (YA4627) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Applications

Application
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
IF-Tissue Info
IF-Tissue: Immunofluorescence-Tissue
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
IHC-F Info
IHC-F: Immunohistochemistry-Frozen
FC Info
FC: Flow Cytometry
Dilution Ratio 1:500-1:2000 1:100 1:50-1:200 1:50-1:200 1:100 1:1000

Product Details

Description

CaMKII Antibody (YA4627) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to CaMKII.

  • Host Rabbit
  • Clonality Monoclonal,Recombinant
  • Species Reactivity
    Human, Mouse, Rat, Monkey, Pig Predicted Reactivity: Pig
    Info
    Note: The predicted reactivity is for reference only and should not be considered a guarantee of product performance.
  • Observed Molecular Weight
    Observed band size: 50 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 54 kDa
Species Reactivity Database
Immunogen

Synthetic peptide within Human CaMKⅡ aa 201-250 / 478.

Purification

Protein A affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3719005

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for CaMKII Antibody (YA4627)
    Western blot analysis of extracts from C6 (lane 2(20μg) and C6 (lane 3(40μg), NIH3T3 (lane 4(20μg) and NIH3T3 (lane 5(40μg) using CaMKII (HY-P84930) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for CaMKII Antibody (YA4627)
    Immunohistochemical analysis of paraffin-embedded mouse brain tissue using CaMKII Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for CaMKII Antibody (YA4627)
    Immunohistochemical analysis of paraffin-embedded mouse brain tissue using CaMKII Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for CaMKII Antibody (YA4627)
    Flow cytometric analysis of 1X106 NIH3T3 cells labeling CaMKII Antibody(HY-P84930, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/1000 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

Background

  • Function

    CaMKII is a Ca2+/calmodulin-dependent serine/threonine kinase that converts intracellular calcium signals into phosphorylation-dependent cellular responses[1]. In excitatory synapses, CaMKII is central to synaptic plasticity, learning, and memory, where it phosphorylates synaptic proteins that regulate receptor trafficking, localization, activity, actin dynamics, translation, and transcription[1]. Mechanistically, Ca2+/calmodulin binding activates the regulatory domain, and CaMKII tools now support studies across cell types and cellular functions[2]. In disease-relevant models, CaMKIIδ expression increases in human heart failure, and CaMKII inhibition acutely improves contractility by reducing sarcoplasmic-reticulum Ca2+ leak and increasing Ca2+ load[3]. Compared with related isoforms, CaMKIIα and CaMKIIβ are largely brain-specific, whereas CaMKIIγ and CaMKIIδ show broader tissue expression[4]. The α and β isoforms mediate synaptic functions underlying learning, memory, and cognition, but CaMKIIβ has high-affinity F-actin binding that distinguishes it from CaMKIIα[4]. Structural comparison of α, β, γ, and δ isoforms documented shared holoenzyme architecture with measurable enzymatic differences among gene products[5]. For experimental applications, KN-93 inhibits CaMKII activity by binding Ca2+/calmodulin, supporting its use as a mechanistic inhibitor with target-engagement considerations[6].

  • Subcellular Localization

    Q13554: Cytoplasm, cytoskeleton; Cytoplasm, cytoskeleton, microtubule organizing center, centrosome; Sarcoplasmic reticulum membrane; Peripheral membrane protein; Cytoplasmic side; Synapse
    Q13555: Sarcoplasmic reticulum membrane; Peripheral membrane protein; Cytoplasmic side
    Q13557: Cell membrane, sarcolemma; Peripheral membrane protein; Cytoplasmic side; Sarcoplasmic reticulum membrane; Peripheral membrane protein; Cytoplasmic side
    Q9UQM7: Synapse; Postsynaptic density; Cell projection, dendritic spine; Cell projection, dendrite

  • Expression


    Tissue_specificity: Q13554: Widely expressed. Expressed in adult and fetal brain. Expression is slightly lower in fetal brain. Expressed in skeletal muscle
    Q13555: Expressed in skeletal muscle
    Q13557: Expressed in cardiac muscle and skeletal muscle. Isoform Delta 3, isoform Delta 2, isoform Delta 8 and isoform Delta 9 are expressed in cardiac muscle. Isoform Delta 11 is expressed in skeletal muscle
    Induction: Q13554: Activity is induced in skeletal muscle during exercise
    Q13555: Activity is induced in skeletal muscle during exercise
    Q13557: Activity is induced in skeletal muscle during exercise

  • Subunit

    Q13554: CAMK2 is composed of 4 different chains: alpha (CAMK2A) , beta (CAMK2B) , gamma (CAMK2G) , and delta (CAMK2D). The different isoforms assemble into homo- or heteromultimeric holoenzymes composed of 12 subunits with two hexameric rings stacked one on top of the other (PubMed:14722083).
    Q13555: CAMK2 is composed of 4 different chains: alpha (CAMK2A) , beta (CAMK2B) , gamma (CAMK2G) , and delta (CAMK2D) .
    Q13557: CAMK2 is composed of 4 different chains: alpha (CAMK2A) , beta (CAMK2B) , gamma (CAMK2G) , and delta (CAMK2D) .
    Q9UQM7: There are 4 genes encoding calcium/calmodulin-dependent protein kinase type II chains: CAMK2A, CAMK2B, CAMK2G and CAMK2D. The corresponding proteins assemble into homo- or heteromultimeric holoenzymes composed of 12 subunits with two hexameric rings stacked one on top of the other (PubMed:14722083, PubMed:29784083).

  • SwissProt ID

    Q13554 / Q13555 / Q13557 / Q9UQM7

  • Gene ID
    815 [NCBI] / 816 [NCBI] / 817 [NCBI] / 818 [NCBI]
  • Synonyms

    Calcium/calmodulin dependent protein kinase II alpha antibody; Calcium/calmodulin dependent protein kinase II beta antibody; Calcium/calmodulin dependent protein kinase II delta antibody; Calcium/calmodulin dependent protein kinase II gamma antibody; Calcium/calmodulin-dependent protein kinase type II subunit alpha antibody; CaM kinase II alpha antibody; CaM kinase II antibody; CaM kinase II beta antibody; CaM kinase II delta antibody; CaM kinase II gamma antibody; CaM kinase II subunit alpha antibody; CaMK-II subunit alpha antibody; CAMK2 antibody; Camk2a antibody; CAMK2B antibody; CAMK2D antibody; CAMK2G antibody; CAMKA antibody; KCC2A_HUMAN antibody; CaMKⅡ

References

[1]. Yasuda R, et al. CaMKII: a central molecular organizer of synaptic plasticity, learning and memory. Nat Rev Neurosci. 2022 Nov;23(11):666-682. [Content Brief]

[2]. Brown CN, et al. Studying CaMKII: Tools and standards. Cell Rep. 2024 Apr 23;43(4):113982. [Content Brief]

[3]. Sossalla S, et al. Inhibition of elevated Ca2+/calmodulin-dependent protein kinase II improves contractility in human failing myocardium. Circ Res. 2010 Oct 29;107(9):1150-61. [Content Brief]

[4]. Cook SG, et al. Analysis of the CaMKIIα and β splice-variant distribution among brain regions reveals isoform-specific differences in holoenzyme formation. Sci Rep. 2018 Apr 3;8(1):5448. [Content Brief]

[5]. Gaertner TR, et al. Comparative analyses of the three-dimensional structures and enzymatic properties of alpha, beta, gamma and delta isoforms of Ca2+-calmodulin-dependent protein kinase II. J Biol Chem. 2004 Mar 26;279(13):12484-94. [Content Brief]

[6]. Wong MH, et al. The KN-93 Molecule Inhibits Calcium/Calmodulin-Dependent Protein Kinase II (CaMKII) Activity by Binding to Ca2+/CaM. J Mol Biol. 2019 Mar 29;431(7):1440-1459. [Content Brief]

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CaMKII Antibody (YA4627) Related Classifications

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis of extracts from C6 (lane 2(20μg) and C6 (lane 3(40μg), NIH3T3 (lane 4(20μg) and NIH3T3 (lane 5(40μg) using CaMKII (HY-P84930) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse brain tissue using CaMKII Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse brain tissue using CaMKII Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Flow cytometric analysis of 1X106 NIH3T3 cells labeling CaMKII Antibody(HY-P84930, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/1000 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

  • CaMKII Antibody (YA4627) Product Image
    1/16
  • Experimental Validation Results for CaMKII Antibody (YA4627)
  • Experimental Validation Results for CaMKII Antibody (YA4627)
  • Experimental Validation Results for CaMKII Antibody (YA4627)
  • Experimental Validation Results for CaMKII Antibody (YA4627)
  • CaMKII Antibody (YA4627) Product Image
  • Experimental Validation Results for CaMKII Antibody (YA4627)
    1/1
    MedchemExpress Validation
     Western blot analysis of extracts from C6 (lane 2(20μg) and C6 (lane 3(40μg), NIH3T3 (lane 4(20μg) and NIH3T3 (lane 5(40μg) using CaMKII (HY-P84930) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for CaMKII Antibody (YA4627)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse brain tissue using CaMKII Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for CaMKII Antibody (YA4627)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse brain tissue using CaMKII Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for CaMKII Antibody (YA4627)
    1/1
    MedChemExpress Validation
     Flow cytometric analysis of 1X106 NIH3T3 cells labeling CaMKII Antibody(HY-P84930, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/1000 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).
  • Experimental Validation Results for CaMKII Antibody (YA4627)
  • Experimental Validation Results for CaMKII Antibody (YA4627)
  • Experimental Validation Results for CaMKII Antibody (YA4627)
  • Experimental Validation Results for CaMKII Antibody (YA4627)
  • CaMKII Antibody (YA4627) Product Image
    MedchemExpress Validation

    Western blot analysis of extracts from C6 (lane 2(20μg) and C6 (lane 3(40μg), NIH3T3 (lane 4(20μg) and NIH3T3 (lane 5(40μg) using CaMKII (HY-P84930) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • CaMKII Antibody (YA4627) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue using CaMKII Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • CaMKII Antibody (YA4627) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue using CaMKII Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • CaMKII Antibody (YA4627) Product Image
    MedChemExpress Validation

    Flow cytometric analysis of 1X106 NIH3T3 cells labeling CaMKII Antibody(HY-P84930, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/1000 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

  • CaMKII Antibody (YA4627) Product Image WB
  • CaMKII Antibody (YA4627) Product Image IHC-P
  • CaMKII Antibody (YA4627) Product Image IHC-P
  • CaMKII Antibody (YA4627) Product Image FC