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HtrA2/Omi Antibody (YA726)(PBS only)

(Synonyms: High temperature requirement protein A2; HTRA 2; HtrA like serine protease; HtrA serine peptidase 2; HtrA; E. coli; homolog of; 2; HtrA2; HTRA2_HUMAN; mitochondrial; Omi stress regulated endoprotease; Omi stress-regulated endoprotease; PARK 13; PARK13; Pr)

HtrA2/Omi Antibody (YA726) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to HtrA2/Omi.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG

  • Application:

    WB, IP

  • Reactivity :

    Human

  • Formulation:

    Supplied in PBS, pH 7.4.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for HtrA2/Omi Antibody (YA726)(PBS only)
  • HtrA2/Omi Antibody (YA726)(PBS only) Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:500-1:1000 1:20

Product Details

Description

HtrA2/Omi Antibody (YA726) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to HtrA2/Omi.

  • Host Mouse
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 37 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 49 kDa
Immunogen

Synthetic peptide corresponding to Human HtrA2/Omi.The exact sequence is proprietary to MCE.

Purification

affinity purified

Conjugation

Non-conjugated

Isotype

IgG

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in PBS, pH 7.4.

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for HtrA2/Omi Antibody (YA726)(PBS only)
    Western blot analysis of extracts from HEK293(lane 2(20ug) , Hela(lane 3(20ug) and Jurkat(lane 4(20ug) using HtrA2 Antibody (HY-P80715) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

Background

  • Function

    HtrA2/Omi serine protease that shows proteolytic activity against a non-specific substrate beta-casein. Promotes apoptosis by either relieving the inhibition of BIRC proteins on caspases, leading to an increase in caspase activity; or by a BIRC inhibition-independent, caspase-independent and serine protease activity-dependent mechanism. Cleaves BIRC6 and relieves its inhibition on CASP3, CASP7 and CASP9, but it is also prone to inhibition by BIRC6. Cleaves THAP5 and promotes its degradation during apoptosis; Seems to be proteolytically inactive[1][2][3][4][5].

  • Subcellular Localization

    Mitochondrion intermembrane space; Mitochondrion membrane; Single-pass membrane protein; Endoplasmic reticulum

  • Expression


    Tissue_specificity:general expression

  • Isoforms & Post-Translational Modification

    O43464 has 4 isomers: O43464-1: 48841 Da (predicted); O43464-2: 38493 Da (predicted); O43464-3: 46382 Da (predicted); O43464-4: 39914 Da (predicted).
    Ubiquitinated by BIRC6; this activity is inhibited by DIABLO/SMAC;Autoproteolytically activated

  • Subunit

    Homotrimer (PubMed:36758104, PubMed:36758105). Interacts with MXI2. Interacts with THAP5 under apoptotic conditions. The mature protein, but not the precursor, binds to BIRC2/c-IAP1, BIRC3/c-IAP2 and XIAP/BIRC4. Interacts with AREL1 (via HECT domain); in the cytoplasm following induction of apoptosis (PubMed:23479728)

  • SwissProt ID

    O43464

  • Gene ID
    27429 [NCBI]
  • Synonyms

    High temperature requirement protein A2; HTRA 2; HtrA like serine protease; HtrA serine peptidase 2; HtrA; E. coli; homolog of; 2; HtrA2; HTRA2_HUMAN; mitochondrial; Omi stress regulated endoprotease; Omi stress-regulated endoprotease; PARK 13; PARK13; Pr

  • Research Field

    Neuroscience

References

[1]. Savopoulos JW, et al. Expression, purification, and functional analysis of the human serine protease HtrA2. Protein Expr Purif. 2000 Jul;19(2):227-34. [Content Brief]

[2]. Bartke T, et al. Dual role of BRUCE as an antiapoptotic IAP and a chimeric E2/E3 ubiquitin ligase. Mol Cell. 2004 Jun 18;14(6):801-11. [Content Brief]

[3]. Hunkeler M, et al. Structures of BIRC6-client complexes provide a mechanism of SMAC-mediated release of caspases. Science. 2023 Mar 17;379(6637):1105-1111. [Content Brief]

[4]. Ehrmann JF, et al. Structural basis for regulation of apoptosis and autophagy by the BIRC6/SMAC complex. Science. 2023 Mar 17;379(6637):1117-1123. [Content Brief]

[5]. Balakrishnan MP, et al. THAP5 is a human cardiac-specific inhibitor of cell cycle that is cleaved by the proapoptotic Omi/HtrA2 protease during cell death. Am J Physiol Heart Circ Physiol. 2009 Aug;297(2):H643-53. [Content Brief]

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HtrA2/Omi Antibody (YA726)(PBS only) Related Classifications

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from HEK293(lane 2(20ug) , Hela(lane 3(20ug) and Jurkat(lane 4(20ug) using HtrA2 Antibody (HY-P80715) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • HtrA2/Omi Antibody (YA726)(PBS only) Product Image
    1/16
  • Experimental Validation Results for HtrA2/Omi Antibody (YA726)(PBS only)
  • HtrA2/Omi Antibody (YA726)(PBS only) Product Image
  • Experimental Validation Results for HtrA2/Omi Antibody (YA726)(PBS only)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from HEK293(lane 2(20ug) , Hela(lane 3(20ug) and Jurkat(lane 4(20ug) using HtrA2 Antibody (HY-P80715) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for HtrA2/Omi Antibody (YA726)(PBS only)
  • HtrA2/Omi Antibody (YA726)(PBS only) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from HEK293(lane 2(20ug) , Hela(lane 3(20ug) and Jurkat(lane 4(20ug) using HtrA2 Antibody (HY-P80715) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • HtrA2/Omi Antibody (YA726)(PBS only) Product Image WB