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Vitamin D Binding Protein Antibody (YA009)

(Synonyms: GC; Vitamin D-binding protein; DBP; VDB; Gc-globulin; Group-specific component)
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Based on 1 Customer Validation

Vitamin D Binding Protein Antibody (YA009) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Vitamin D Binding Protein.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, ICC/IF

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol and 0.05% BSA. Preservative: 0.01% Sodium azide

  • Conjugation:
    Non-conjugated
ALL WB ICC
  • Experimental Validation Results for Vitamin D Binding Protein Antibody (YA009)
  • Experimental Validation Results for Vitamin D Binding Protein Antibody (YA009)
  • Experimental Validation Results for Vitamin D Binding Protein Antibody (YA009)
  • Vitamin D Binding Protein Antibody (YA009) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
Dilution Ratio 1:500-1:1000 1:50-1:200

Product Details

Description

Vitamin D Binding Protein Antibody (YA009) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Vitamin D Binding Protein.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 53 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 53 kDa
Species Reactivity Database
Immunogen

Synthetic peptide corresponding to Human Vitamin D Binding protein aa255-265.

Sensitivity

Endogenous

Purification

affinity purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3103003

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol and 0.05% BSA. Preservative: 0.01% Sodium azide

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Vitamin D Binding Protein Antibody (YA009)
    Western blot analysis of extracts from NIH/3T3(lane 2(20ug) , Hela(lane 3(20ug) and HEK293(lane 4(20ug) using Vitamin D Binding Protein Antibody (HY-P80930) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Vitamin D Binding Protein Antibody (YA009)
    Immunocytochemistry analysis of Hela cells labeling Vitamin D Binding Protein with Vitamin D Binding Protein Antibody (HY-P80930) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with Vitamin D Binding Protein Antibody (HY-P80930) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Vitamin D Binding Protein Antibody (YA009)
    Immunocytochemistry analysis of Hela cells labeling Vitamin D Binding Protein with Vitamin D Binding Protein Antibody (HY-P80930) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with Vitamin D Binding Protein Antibody (HY-P80930) at 1/200 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

  • Function

    Vitamin D Binding Protein is involved in vitamin D transport and storage, scavenging of extracellular G-actin, enhancement of the chemotactic activity of C5 alpha for neutrophils in inflammation and macrophage activation

  • Subcellular Localization

    Secreted

  • Expression


    Tissue_specificity:Expressed in the liver. Found in plasma, ascites, cerebrospinal fluid and urine

  • Isoforms & Post-Translational Modification

    P02774 has 3 isomers: P02774-1: 52918 Da (predicted); P02774-2: 39496 Da (predicted); P02774-3: 55077 Da (predicted).
    Allele GC*1S is O-glycosylated at Thr-436 (PubMed:20079467). The trisaccharide sugar moiety can be modified by the successive removal of neuraminic acid and galactose leaving an O-mceeN-acetyl-galactosamine. This conversion is thought to produce a macrophage-activating factor (Gc-MAF). Only a minor proportion of plasma GC is O-glycosylated (PubMed:17360250). The potential N-glycosylation site predicted at Asn-288 is thought to be nonglycosylated

  • Subunit

    Associates with membrane-bound immunoglobulin on the surface of B-lymphocytes and with IgG Fc receptor on the membranes of T-lymphocytes.

  • SwissProt ID

    P02774

  • Gene ID
    2638 [NCBI]
  • Synonyms

    GC; Vitamin D-binding protein; DBP; VDB; Gc-globulin; Group-specific component

  • Research Field

    Signal Transduction

Vitamin D Binding Protein Antibody (YA009) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis of extracts from NIH/3T3(lane 2(20ug) , Hela(lane 3(20ug) and HEK293(lane 4(20ug) using Vitamin D Binding Protein Antibody (HY-P80930) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling Vitamin D Binding Protein with Vitamin D Binding Protein Antibody (HY-P80930) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with Vitamin D Binding Protein Antibody (HY-P80930) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling Vitamin D Binding Protein with Vitamin D Binding Protein Antibody (HY-P80930) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with Vitamin D Binding Protein Antibody (HY-P80930) at 1/200 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Vitamin D Binding Protein Antibody (YA009) Product Image
    1/16
  • Experimental Validation Results for Vitamin D Binding Protein Antibody (YA009)
  • Experimental Validation Results for Vitamin D Binding Protein Antibody (YA009)
  • Experimental Validation Results for Vitamin D Binding Protein Antibody (YA009)
  • Vitamin D Binding Protein Antibody (YA009) Product Image
  • Experimental Validation Results for Vitamin D Binding Protein Antibody (YA009)
    1/1
    MedchemExpress Validation
     Western blot analysis of extracts from NIH/3T3(lane 2(20ug) , Hela(lane 3(20ug) and HEK293(lane 4(20ug) using Vitamin D Binding Protein Antibody (HY-P80930) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Vitamin D Binding Protein Antibody (YA009)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling Vitamin D Binding Protein with Vitamin D Binding Protein Antibody (HY-P80930) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with Vitamin D Binding Protein Antibody (HY-P80930) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Vitamin D Binding Protein Antibody (YA009)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling Vitamin D Binding Protein with Vitamin D Binding Protein Antibody (HY-P80930) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with Vitamin D Binding Protein Antibody (HY-P80930) at 1/200 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Vitamin D Binding Protein Antibody (YA009)
  • Experimental Validation Results for Vitamin D Binding Protein Antibody (YA009)
  • Experimental Validation Results for Vitamin D Binding Protein Antibody (YA009)
  • Vitamin D Binding Protein Antibody (YA009) Product Image
    MedchemExpress Validation

    Western blot analysis of extracts from NIH/3T3(lane 2(20ug) , Hela(lane 3(20ug) and HEK293(lane 4(20ug) using Vitamin D Binding Protein Antibody (HY-P80930) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • Vitamin D Binding Protein Antibody (YA009) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of Hela cells labeling Vitamin D Binding Protein with Vitamin D Binding Protein Antibody (HY-P80930) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with Vitamin D Binding Protein Antibody (HY-P80930) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Vitamin D Binding Protein Antibody (YA009) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of Hela cells labeling Vitamin D Binding Protein with Vitamin D Binding Protein Antibody (HY-P80930) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with Vitamin D Binding Protein Antibody (HY-P80930) at 1/200 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Vitamin D Binding Protein Antibody (YA009) Product Image WB
  • Vitamin D Binding Protein Antibody (YA009) Product Image ICC
  • Vitamin D Binding Protein Antibody (YA009) Product Image ICC