Vitamin D Binding Protein Antibody (YA009)

Cat. No.: HY-P80930: Data Sheet COA
SDS
User Guide for Antibodies
FAQs
Technical Support

Vitamin D Binding Protein Antibody (YA009) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Vitamin D Binding Protein.

For research use only. We do not sell to patients.

Size	Stock	Quantity
Free Sample	Apply now
10 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
250 μL	Get quote

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Vitamin D Binding Protein Antibody (YA009) Featured Recommendations:

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
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Background
Documentation

Description

Vitamin D Binding Protein Antibody (YA009) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Vitamin D Binding Protein.

Host

Rabbit

Clonality

Recombinant, Monoclonal

Molecular Weight

Predicted band size: 53 kDa;

Observed band size: 53 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

P02774

Gene ID

2638 [NCBI]

Immunogen

Synthetic peptide corresponding to Human Vitamin D Binding protein aa255-265.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:50-1:200

Sensitivity	Endogenous	Purity	affinity purified
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol and 0.05% BSA. Preservative: 0.01% Sodium azide

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Verification Image

Western blot analysis of extracts from NIH/3T3(lane 2(20ug) , Hela(lane 3(20ug) and HEK293(lane 4(20ug) using Vitamin D Binding Protein Antibody (HY-P80930) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

Background

Function：Involved in vitamin D transport and storage, scavenging of extracellular G-actin, enhancement of the chemotactic activity of C5 alpha for neutrophils in inflammation and macrophage activation

Subcellular Localization：Secreted

Expression：
Tissue_specificity:Expressed in the liver. Found in plasma, ascites, cerebrospinal fluid and urine

Isoforms & Post-Translational Modification：P02774 has 3 isomers: P02774-1: 52918 Da (predicted); P02774-2: 39496 Da (predicted); P02774-3: 55077 Da (predicted).
Allele GC*1S is O-glycosylated at Thr-436 (PubMed:20079467). The trisaccharide sugar moiety can be modified by the successive removal of neuraminic acid and galactose leaving an O-mceeN-acetyl-galactosamine. This conversion is thought to produce a macrophage-activating factor (Gc-MAF). Only a minor proportion of plasma GC is O-glycosylated (PubMed:17360250). The potential N-glycosylation site predicted at Asn-288 is thought to be nonglycosylated

Subunit：Associates with membrane-bound immunoglobulin on the surface of B-lymphocytes and with IgG Fc receptor on the membranes of T-lymphocytes.

RRID

AB_3103003

Database

Entrez Gene: 2638 Human ; 14473 Mouse ;

SwissProt: P02774 Human ; P21614 Mouse ;

OMIM: 139200 Human

Research Field

Signal Transduction

Synonyms

GC; Vitamin D-binding protein; DBP; VDB; Gc-globulin; Group-specific component

Documentation

Select Batch:

Data Sheet (232 KB) SDS (251 KB)

COA (206 KB)

User Guide for Antibodies (1077 KB)

Vitamin D Binding Protein Antibody (YA009) Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Vitamin D Binding Protein Antibody (YA009)

Vitamin D Binding Protein Antibody (YA009) Featured Recommendations:

Product Detail

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Background

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Vitamin D Binding Protein Antibody (YA009) Related Classifications

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