CD53 Antibody (YA3854)(PBS only)

Cat. No.: HY-P84157A: Data Sheet User Guide for Antibodies
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CD53 Antibody (YA3854) is a Mouse-derived and non-conjugated IgG2a monoclonal antibody, targeting to CD53.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

CD53 Antibody (YA3854) is a Mouse-derived and non-conjugated IgG2a monoclonal antibody, targeting to CD53.

Host

Mouse

Molecular Weight

Predicted band size: 24 kDa;

Observed band size: 35-55 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

P19397

Gene ID

963 [NCBI]

Immunogen

Purified recombinant fragment of human CD53 (AA: extra mix) expressed in E. Coli.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:2000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:200-1:1000
FC FC: Flow Cytometry	1:200-1:400
ELISA ELISA: Enzyme Linked Immunosorbent Assay	1:10000

Purity	affinity purified.	Conjugation	Non-conjugated
Isotype	IgG2a

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Structural component of specialized membrane microdomains known as tetraspanin-enriched microdomains (TERMs), which act as platforms for receptor clustering and signaling (PubMed:28487417). Participates thereby in diverse biological functions such as cell signal transduction, adhesion, migration and protein trafficking (PubMed:32974937, PubMed:35767951). Plays a role in the activation of monocytes and B-cells (PubMed:8335905). Acts as an essential regulator of B-cell development by promoting interleukin-7 receptor/IL7R signaling (By similarity). Also promotes, in B-cells, the BCR signaling by recruiting PKC to the plasma membrane in order to phosphorylate its substrates (PubMed:28487417). Plays an essential role in B- and T-cells homing to lymph nodes by stabilizing L-selectin/SELL cell surface expression (By similarity). Also mediates metabolic and inflammatory functions in hepatocytes and adipose tissue by promoting TNF-alpha and LPS signaling independent of the immune compartment (By similarity)

Subcellular Localization：Cell membrane; Cell junction; Membrane; Multi-pass membrane protein; Synapse

Expression：
Tissue_specificity:B cells, monocytes, macrophages, neutrophils, single-positive (CD4 or CD8) thymocytes, and peripheral T cells

Subunit：Interacts with SCIMP (PubMed:21930792). Interacts with CD45/PTPRC (PubMed:35767951). Interacts with IL7R (By similarity). Interacts with RBL2 and PPP2CA (By similarity)

Synonyms

MOX44; TSPAN25

Documentation

User Guide for Antibodies (1077 KB)