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GFPT2 Antibody (YA2914)

(Synonyms: GFAT; GFAT2; GFAT 2)
Customer Review

Based on 1 Customer Validation

GFPT2 Antibody (YA2914) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to GFPT2.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, ICC/IF, IP

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for GFPT2 Antibody (YA2914)
  • GFPT2 Antibody (YA2914) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:500-1:1000 1:50-1:200 1:20

Product Details

Description

GFPT2 Antibody (YA2914) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to GFPT2.

  • Host Rabbit
  • Clonality Recombinant, Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 77 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 77 kDa
Species Reactivity Database
Immunogen

Recombinant protein of human GFPT2

Sensitivity

Endogenous

Purification

Affinity Purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3105284

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for GFPT2 Antibody (YA2914)
    Western blot analysis of extracts from A375 (lane 2(20μg)) ,Mouse Heart (lane 3(20μg)) and Mouse Heart (lane 3(20μg)) using GFPT2 Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (beta Actin, HY-P83730, 1/5000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit/Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

Background

  • Function

    GFPT2 controls the flux of glucose into the hexosamine pathway. Most likely involved in regulating the availability of precursors for N- and O-linked glycosylation of proteins

  • Expression


    Tissue_specificity:Highest levels of expression in heart, placenta, and spinal cord

  • SwissProt ID

    O94808

  • Gene ID
    9945 [NCBI]
  • Synonyms

    GFAT; GFAT2; GFAT 2

  • Research Field

    Signal Transduction

GFPT2 Antibody (YA2914) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from A375 (lane 2(20μg)) ,Mouse Heart (lane 3(20μg)) and Mouse Heart (lane 3(20μg)) using GFPT2 Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (beta Actin, HY-P83730, 1/5000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit/Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • GFPT2 Antibody (YA2914) Product Image
    1/16
  • Experimental Validation Results for GFPT2 Antibody (YA2914)
  • GFPT2 Antibody (YA2914) Product Image
  • Experimental Validation Results for GFPT2 Antibody (YA2914)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from A375 (lane 2(20μg)) ,Mouse Heart (lane 3(20μg)) and Mouse Heart (lane 3(20μg)) using GFPT2 Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (beta Actin, HY-P83730, 1/5000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit/Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for GFPT2 Antibody (YA2914)
  • GFPT2 Antibody (YA2914) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from A375 (lane 2(20μg)) ,Mouse Heart (lane 3(20μg)) and Mouse Heart (lane 3(20μg)) using GFPT2 Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (beta Actin, HY-P83730, 1/5000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit/Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • GFPT2 Antibody (YA2914) Product Image WB