Phospho-Ephrin B1 (Tyr317) Antibody
(Synonyms: EFL3, EPLG2, LERK2, EFNB1, Ephrin-B1, EFL-3, ELK ligand, EPH-related receptor tyrosine kinase ligand 2, ELK-L, LERK-2)Phospho-Ephrin B1 (Tyr317) Antibody is a Rabbit-derived and non-conjugated IgG Polyclonal antibody, targeting to Phospho-Ephrin B1 (Tyr317).
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, IHC-P
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Reactivity :
Human, Mouse, Rat
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Formulation:
Supplied in PBS (pH 7.4), containing 30% glycerol, and 0.01% sodium azide.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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IHC-P
IHC-P: Immunohistochemistry-Paraffin
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|---|---|---|
| Dilution Ratio | 1:1000-2000 | 1:100-200 |
Product Details
Phospho-Ephrin B1 (Tyr317) Antibody is a Rabbit-derived and non-conjugated IgG Polyclonal antibody, targeting to Phospho-Ephrin B1 (Tyr317).
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Host Rabbit
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Clonality Polyclonal
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Species ReactivityHuman, Mouse, Rat
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Observed Molecular WeightObserved band size: 45 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 38 kDa
Synthetic phosphopeptide corresponding to residues surrounding Y317 of human Ephrin B1 protein.
Endogenous
affinity purified.
Non-conjugated
Phosphorylated
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in PBS (pH 7.4), containing 30% glycerol, and 0.01% sodium azide.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
Ephrin B1 is a Cell surface transmembrane ligand for Eph receptors, a family of receptor tyrosine kinases which are crucial for migration, repulsion and adhesion during neuronal, vascular and epithelial development. Binding to Eph receptors residing on adjacent cells leads to contact-dependent bidirectional signaling into neighboring cells. Shows high affinity for the receptor tyrosine kinase EPHB1/ELK. Can also bind EPHB2 and EPHB3. Binds to, and induces collapse of, commissural axons/growth cones in vitro (By similarity). May play a role in constraining the orientation of longitudinally projecting axons (By similarity)[1][2].
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Subcellular Localization
Cell membrane; Membrane raft
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Expression
Tissue_Specificity: Widely expressed. Detected in both neuronal and non-neuronal tissues. Seems to have particularly strong expression in retina, sciatic nerve, heart and spinal cord.
Induction: Up-regulated in response to TNF. -
Isoforms & Post-Translational Modification
Phospho-Ephrin B1 has an amino acid length of 346, molecular weight is 38007 Da.
Inducible phosphorylation of tyrosine residues in the cytoplasmic domain. -
Subunit
Interacts (via PDZ-binding motif) with GRIP1 and GRIP2 (via PDZ domain 6).
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SwissProt ID
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Synonyms
EFL3, EPLG2, LERK2, EFNB1, Ephrin-B1, EFL-3, ELK ligand, EPH-related receptor tyrosine kinase ligand 2, ELK-L, LERK-2
Documentation
References
[1]. Davis S, et al. Ligands for EPH-related receptor tyrosine kinases that require membrane attachment or clustering for activity. Science. 1994 Nov 4;266(5186):816-9. [Content Brief]
[2]. Beckmann MP, et al. Molecular characterization of a family of ligands for eph-related tyrosine kinase receptors. EMBO J. 1994 Aug 15;13(16):3757-62. [Content Brief]
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