TRPM4 Antibody (YA7894)(PBS only)

Cat. No.: HY-P88210A: Data Sheet User Guide for Antibodies
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TRPM4 Antibody (YA7894) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to TRPM4.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

TRPM4 Antibody (YA7894) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to TRPM4.

Host

Mouse

Molecular Weight

Predicted band size: 134.3 kDa

Species Reactivity

Human

SwissProt ID

Q8TD43

Immunogen

Full-length protein expressed in 293T cell transfected with human TRPM4 expression vector

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:1000-2000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:50-250

Purity	Affinity purified	Conjugation	Non-conjugated
Isotype	IgG1

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Calcium-activated selective cation channel that mediates membrane depolarization (PubMed:12015988, PubMed:12842017, PubMed:29211723, PubMed:30528822). While it is activated by increase in intracellular Ca(2+), it is impermeable to it (PubMed:12015988). Mediates transport of monovalent cations (Na(+) > K(+) > Cs(+) > Li(+)), leading to depolarize the membrane (PubMed:12015988). It thereby plays a central role in cadiomyocytes, neurons from entorhinal cortex, dorsal root and vomeronasal neurons, endocrine pancreas cells, kidney epithelial cells, cochlea hair cells etc. Participates in T-cell activation by modulating Ca(2+) oscillations after T lymphocyte activation, which is required for NFAT-dependent IL2 production. Involved in myogenic constriction of cerebral arteries. Controls insulin secretion in pancreatic beta-cells. May also be involved in pacemaking or could cause irregular electrical activity under conditions of Ca(2+) overload. Affects T-helper 1 (Th1) and T-helper 2 (Th2) cell motility and cytokine production through differential regulation of calcium signaling and NFATC1 localization. Enhances cell proliferation through up-regulation of the beta-catenin signaling pathway. Plays a role in keratinocyte differentiation (PubMed:30528822)|Lacks channel activity

Subcellular Localization：Cell membrane,Endoplasmic reticulum,Golgi apparatus,Cell membrane,Endoplasmic reticulum,Golgi apparatus

Expression：
Tissue_Specificity: Widely expressed with a high expression in intestine and prostate. In brain, it is both expressed in whole cerebral arteries and isolated vascular smooth muscle cells. Prominently expressed in Purkinje fibers. Expressed at higher levels in T-helper 2 (Th2) cells as compared to T-helper 1 (Th1) cells. Expressed in keratocytes (PubMed:30528822)

Isoforms & Post-Translational Modification：Q8TD43 has three isomers: Q8TD43-1: 134301 Da (predicted); Q8TD43-2: 115566 Da (predicted); Q8TD43-3: 118630 Da (predicted).
Phosphorylation by PKC leads to increase the sensitivity to Ca(2+)丨Sumoylated

Subunit：Homotetramer

Synonyms

hTRPM4; LTrpC4; PFHB1B; TRPM4B

Documentation

User Guide for Antibodies (1077 KB)